Transcript: Human NM_001319179.1

Homo sapiens guanylate binding protein 3 (GBP3), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-05
Taxon:
Homo sapiens (human)
Gene:
GBP3 (2635)
Length:
3137
CDS:
529..2079

Additional Resources:

NCBI RefSeq record:
NM_001319179.1
NBCI Gene record:
GBP3 (2635)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001319179.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000370506 TGGTGGAGGAAATGCAAATAA pLKO_005 1799 CDS 100% 15.000 10.500 N GBP3 n/a
2 TRCN0000365397 AGCCTAGTGCTGACCTATATC pLKO_005 1168 CDS 100% 13.200 9.240 N GBP3 n/a
3 TRCN0000365327 GGAACAGGCCCGAGTACTAAA pLKO_005 1950 CDS 100% 13.200 9.240 N GBP3 n/a
4 TRCN0000370444 GGAGGCCACTGAAGTCTATAT pLKO_005 1371 CDS 100% 13.200 9.240 N GBP3 n/a
5 TRCN0000365398 GCATAAGCTAAAGATCTAAAC pLKO_005 2061 CDS 100% 10.800 7.560 N GBP3 n/a
6 TRCN0000148690 CCCAAGGCATAACTGAAACAA pLKO.1 2106 3UTR 100% 5.625 3.938 N GBP3 n/a
7 TRCN0000146617 CTCACTAGTAAACTTCAGGAA pLKO.1 1933 CDS 100% 0.000 0.000 N GBP3 n/a
8 TRCN0000370442 CTCTGGGCTCCACAGTGAAAT pLKO_005 488 5UTR 100% 13.200 7.920 N GBP3 n/a
9 TRCN0000147062 CCAAGGCATAACTGAAACAAT pLKO.1 2107 3UTR 100% 5.625 3.375 N GBP3 n/a
10 TRCN0000148294 CCTGAATTTGTGCAACAAGTA pLKO.1 1063 CDS 100% 4.950 2.475 Y GBP3 n/a
11 TRCN0000148170 GCAGACATACTTGAAATCCAA pLKO.1 1668 CDS 100% 3.000 1.500 Y GBP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001319179.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06263 pDONR223 100% 77.9% 73.7% None (many diffs) n/a
2 ccsbBroad304_06263 pLX_304 0% 77.9% 73.7% V5 (many diffs) n/a
3 TRCN0000466638 CCGTTGTCGCAGGTAGGATGGTGT pLX_317 25.6% 77.9% 73.7% V5 (many diffs) n/a
4 ccsbBroadEn_10841 pDONR223 100% 35.4% 35.4% None 0_1ins237;633_1548delinsC n/a
5 ccsbBroad304_10841 pLX_304 0% 35.4% 35.4% V5 0_1ins237;633_1548delinsC n/a
6 TRCN0000465445 GTGGGTAACAGGAGCAGAAACGGG pLX_317 24.2% 35.4% 35.4% V5 0_1ins237;633_1548delinsC n/a
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