Transcript: Human NM_001321675.2

Homo sapiens leucine rich repeat containing 28 (LRRC28), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
LRRC28 (123355)
Length:
6295
CDS:
565..1668

Additional Resources:

NCBI RefSeq record:
NM_001321675.2
NBCI Gene record:
LRRC28 (123355)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321675.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000432959 ACCTCACTGTGGACCGAAATC pLKO_005 1046 CDS 100% 10.800 15.120 N LRRC28 n/a
2 TRCN0000005131 CGGTGTAGTGAGCCTATGTTT pLKO.1 1513 CDS 100% 5.625 7.875 N LRRC28 n/a
3 TRCN0000005129 GCCTATGTTTACCATCGTCTA pLKO.1 1524 CDS 100% 4.050 5.670 N LRRC28 n/a
4 TRCN0000424396 ACTATACCTGCACTCAAATAA pLKO_005 771 CDS 100% 15.000 12.000 N LRRC28 n/a
5 TRCN0000423131 CCATCTTATCTGTACAATAAA pLKO_005 1222 CDS 100% 15.000 10.500 N LRRC28 n/a
6 TRCN0000434516 ACCAATCGTTTGCTAACTTTA pLKO_005 991 CDS 100% 13.200 9.240 N LRRC28 n/a
7 TRCN0000425165 TTTGACCTGCTGAGTTGATAA pLKO_005 1651 CDS 100% 13.200 9.240 N LRRC28 n/a
8 TRCN0000434786 ACTCCAATGTCTGGATCTTAG pLKO_005 831 CDS 100% 10.800 7.560 N LRRC28 n/a
9 TRCN0000005130 GCTTTACGTCATCTTCGATTA pLKO.1 898 CDS 100% 10.800 7.560 N LRRC28 n/a
10 TRCN0000010924 GATAACAACATTCACCTGAAA pLKO.1 1195 CDS 100% 4.950 3.465 N LRRC28 n/a
11 TRCN0000010923 CCAGTCCAGCACACTCTTCCA pLKO.1 1721 3UTR 100% 0.880 0.616 N LRRC28 n/a
12 TRCN0000256748 GGCAGGAGAATTGCTTGAATC pLKO_005 5587 3UTR 100% 10.800 5.400 Y SMIM11A n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2883 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2883 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321675.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04772 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04772 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466194 TCCCGTCAACCGGTGAGCTGGTCA pLX_317 35.2% 100% 100% V5 n/a
4 ccsbBroadEn_13098 pDONR223 100% 22.8% 20.2% None (many diffs) n/a
5 ccsbBroad304_13098 pLX_304 0% 22.8% 20.2% V5 (many diffs) n/a
6 TRCN0000468959 TCCTAGGGACCACCATATTTTTAG pLX_317 100% 22.8% 20.2% V5 (many diffs) n/a
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