Construct: ORF TRCN0000468959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011114.1_s317c1
- Derived from:
- ccsbBroadEn_13098
- DNA Barcode:
- TCCTAGGGACCACCATATTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LRRC28 (123355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468959
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 123355 | LRRC28 | leucine rich repeat contain... | XM_006720389.4 | 33.7% | 30% | (many diffs) |
| 2 | human | 123355 | LRRC28 | leucine rich repeat contain... | XM_017021914.1 | 33.7% | 30% | (many diffs) |
| 3 | human | 123355 | LRRC28 | leucine rich repeat contain... | XM_011521221.3 | 30.2% | 26.8% | (many diffs) |
| 4 | human | 123355 | LRRC28 | leucine rich repeat contain... | NM_001321677.2 | 28% | 31.2% | (many diffs) |
| 5 | human | 123355 | LRRC28 | leucine rich repeat contain... | NM_001284400.3 | 26.8% | 23.8% | (many diffs) |
| 6 | human | 123355 | LRRC28 | leucine rich repeat contain... | XM_011521220.2 | 24.4% | 21.6% | (many diffs) |
| 7 | human | 123355 | LRRC28 | leucine rich repeat contain... | NM_001321676.2 | 23.1% | 20.4% | (many diffs) |
| 8 | human | 123355 | LRRC28 | leucine rich repeat contain... | NM_001321675.2 | 22.8% | 20.2% | (many diffs) |
| 9 | human | 123355 | LRRC28 | leucine rich repeat contain... | NM_144598.5 | 22.8% | 20.2% | (many diffs) |
| 10 | human | 123355 | LRRC28 | leucine rich repeat contain... | XM_011521218.2 | 22.8% | 20.2% | (many diffs) |
| 11 | human | 123355 | LRRC28 | leucine rich repeat contain... | XR_001751086.2 | 16.6% | (many diffs) | |
| 12 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135759.2 | 4.5% | (many diffs) | |
| 13 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135753.2 | 4.4% | (many diffs) | |
| 14 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135757.2 | 4.3% | (many diffs) | |
| 15 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135756.2 | 4.3% | (many diffs) | |
| 16 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135758.2 | 4.2% | (many diffs) | |
| 17 | human | 123355 | LRRC28 | leucine rich repeat contain... | NR_135755.2 | 4% | (many diffs) | |
| 18 | mouse | 67867 | Lrrc28 | leucine rich repeat contain... | NM_027413.1 | 52.7% | 45.8% | (many diffs) |
| 19 | mouse | 67867 | Lrrc28 | leucine rich repeat contain... | XM_006541130.3 | 27.4% | 23.9% | (many diffs) |
| 20 | mouse | 67867 | Lrrc28 | leucine rich repeat contain... | NM_175124.5 | 22.3% | 19.4% | (many diffs) |
| 21 | mouse | 67867 | Lrrc28 | leucine rich repeat contain... | XR_391360.3 | 8.6% | (many diffs) | |
| 22 | mouse | 67867 | Lrrc28 | leucine rich repeat contain... | NR_028143.1 | 8.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 213
- ORF end:
- 492
- ORF length:
- 279
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCATGGCGTC CGAACTTTGT AAGACGATCT 241 CTGTGGCAAG GCTAGAAAAG CACAAGAATT TGTTCTTAAA TTATAGGAAT CTGCACCATT 301 TTCCATTGGA GTTACTGAAA GATGAGGGAC TGCAGTACTT GGAGAGACTC TATATGAAAA 361 GGAACTCCCT GACATCCTTG CCAGAAAACC TTGCTCAGAA GCTTCCAAAC CTTGTGGAAC 421 TGAAGACAAC TGTTAGTTTT GTGGCTTACT GCTGCTCCAC CCAGTGTCTG CAGACTTTTG 481 ACCTGCTGAG TTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCC TAGGGACCAC CATATTTTTA 661 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t