Transcript: Human NM_001369547.1

Homo sapiens neurotrophic receptor tyrosine kinase 2 (NTRK2), transcript variant j, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
NTRK2 (4915)
Length:
7100
CDS:
491..1906

Additional Resources:

NCBI RefSeq record:
NM_001369547.1
NBCI Gene record:
NTRK2 (4915)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148065 TGAATGGAATGCACCAGTGG pXPR_003 TGG 899 63% 9 0.3562 NTRK2 NTRK2 76468
2 BRDN0001146241 ACGTCACTGATAAAACCGGT pXPR_003 CGG 1278 90% 11 -0.2872 NTRK2 NTRK2 76467
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369547.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273160 CCTTAAGGATAACTAACATTT pLKO_005 1236 CDS 100% 13.200 18.480 N NTRK2 n/a
2 TRCN0000197207 GCGCTTCAGTGGTTCTATAAC pLKO.1 1430 CDS 100% 13.200 18.480 N NTRK2 n/a
3 TRCN0000284928 ATCGTGGCATTTCCGAGATTG pLKO_005 644 CDS 100% 10.800 15.120 N NTRK2 n/a
4 TRCN0000195114 CCTAATATGTATTGGGATGTT pLKO.1 1166 CDS 100% 4.950 6.930 N NTRK2 n/a
5 TRCN0000002243 CCAACTATCACATTTCTCGAA pLKO.1 1349 CDS 100% 2.640 2.112 N NTRK2 n/a
6 TRCN0000273089 TCCTGGTGGGCAATCCATTTA pLKO_005 921 CDS 100% 13.200 9.240 N NTRK2 n/a
7 TRCN0000196299 GACTGAGAAATCTGACAATTG pLKO.1 765 CDS 100% 10.800 7.560 N NTRK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369547.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01113 pDONR223 100% 98.4% 97.4% None (many diffs) n/a
2 ccsbBroad304_01113 pLX_304 0% 98.4% 97.4% V5 (many diffs) n/a
3 ccsbBroadEn_14721 pDONR223 0% 56.9% 56.5% None (many diffs) n/a
4 ccsbBroad304_14721 pLX_304 0% 56.9% 56.5% V5 (many diffs) n/a
5 TRCN0000480309 ACAATAAGTGTCCCAGTTAAGTTT pLX_317 14.3% 56.9% 56.5% V5 (many diffs) n/a
6 TRCN0000488063 CTCACTGTCGAGCAACCATACTGA pLX_317 13% 56.9% 56.5% V5 (many diffs) n/a
7 TRCN0000488161 TATCAGACTTTGGGGGGGCAAAAC pLX_317 12.7% 56.9% 56.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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