Transcript: Human NM_006696.4

Homo sapiens bromodomain containing 8 (BRD8), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
BRD8 (10902)
Length:
3193
CDS:
17..2872

Additional Resources:

NCBI RefSeq record:
NM_006696.4
NBCI Gene record:
BRD8 (10902)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006696.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229926 ATCCGAGAGAAGCTATGTTTA pLKO_005 77 CDS 100% 13.200 18.480 N BRD8 n/a
2 TRCN0000222186 CGCAGTTGATTATGCAAACAT pLKO.1 2676 CDS 100% 5.625 7.875 N BRD8 n/a
3 TRCN0000222183 CGTGACATTATGCTGATGTTT pLKO.1 2555 CDS 100% 5.625 7.875 N BRD8 n/a
4 TRCN0000222184 GCCGAAATAGTAGCTGGAGTT pLKO.1 1799 CDS 100% 4.050 5.670 N BRD8 n/a
5 TRCN0000229927 AGATGTTATTGTTCGGAAATT pLKO_005 295 CDS 100% 13.200 9.240 N BRD8 n/a
6 TRCN0000229928 CTATGGATTTGTCAACTATTA pLKO_005 2487 CDS 100% 13.200 9.240 N BRD8 n/a
7 TRCN0000219077 CTGAATTTCAGCGTGACATTA pLKO_005 2544 CDS 100% 13.200 9.240 N BRD8 n/a
8 TRCN0000229929 GAGAGTTGGAGTAGCACAATT pLKO_005 3017 3UTR 100% 13.200 9.240 N BRD8 n/a
9 TRCN0000222185 CGAGTTGAAGAACTAAAGAAA pLKO.1 326 CDS 100% 5.625 3.938 N BRD8 n/a
10 TRCN0000096646 GCCTATGGATTTGTCAACTAT pLKO.1 2485 CDS 100% 5.625 3.938 N Brd8 n/a
11 TRCN0000301665 GCCTATGGATTTGTCAACTAT pLKO_005 2485 CDS 100% 5.625 3.938 N Brd8 n/a
12 TRCN0000222187 GCTGTGTCTTACACAGGTGAA pLKO.1 1442 CDS 100% 0.405 0.284 N BRD8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006696.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487955 ATTGGCGTGTGCGCATAAAGAAGG pLX_317 7.3% 100% 100% V5 (not translated due to prior stop codon) n/a
2 ccsbBroadEn_11567 pDONR223 100% 96.7% 96.7% None 1_93del n/a
3 ccsbBroad304_11567 pLX_304 0% 96.7% 96.7% V5 1_93del n/a
4 TRCN0000472489 ACAACACATTCTTTTCTCCGTATC pLX_317 5.6% 96.7% 96.7% V5 1_93del n/a
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