Construct: ORF TRCN0000487955
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021886.1_s317c1
- DNA Barcode:
- ATTGGCGTGTGCGCATAAAGAAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- BRD8 (10902)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000487955
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10902 | BRD8 | bromodomain containing 8 | NM_006696.4 | 100% | 100% | |
| 2 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001164326.1 | 91% | 91% | 1003_1004ins210;2541_2542ins45 |
| 3 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300961.2 | 90.3% | 88.7% | 0_1ins53;63_67delGGTAA;593_594ins219 |
| 4 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300962.2 | 88% | 85.4% | 0_1ins53;63_64ins70;518_519ins219 |
| 5 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300966.2 | 79.6% | 78.8% | (many diffs) |
| 6 | human | 10902 | BRD8 | bromodomain containing 8 | NM_139199.2 | 66.4% | 65.5% | (many diffs) |
| 7 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_030147.3 | 91.4% | 95% | (many diffs) |
| 8 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526355.3 | 89.8% | 93.4% | (many diffs) |
| 9 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526354.3 | 89.3% | 92.3% | (many diffs) |
| 10 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_001289607.1 | 84.5% | 88.1% | (many diffs) |
| 11 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_001289606.1 | 83.8% | 87.4% | (many diffs) |
| 12 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526358.3 | 83% | 86.5% | (many diffs) |
| 13 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247038.2 | 82.5% | 85.4% | (many diffs) |
| 14 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247042.1 | 82% | 83.1% | (many diffs) |
| 15 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247039.2 | 81.8% | 84.8% | (many diffs) |
| 16 | mouse | 78656 | Brd8 | bromodomain containing 8 | XR_001782404.1 | 75.2% | (many diffs) | |
| 17 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247040.2 | 75% | 77.9% | (many diffs) |
| 18 | mouse | 78656 | Brd8 | bromodomain containing 8 | XR_001782405.1 | 70.9% | (many diffs) | |
| 19 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526359.3 | 67.9% | 68.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2922
- ORF length:
- 2853
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgacggga acgggcaaac acaagctgct aagcactggc cccacagagc 121 catggtccat ccgagagaag ctatgtttag catcttctgt catgagaagt ggcgatcaaa 181 attgggtatc agttagcaga gcaatcaagc cctttgcaga acctggccgc cctccagact 241 ggttctctca aaaacattgt gcttcccagt actcggagct tttagagacc actgagacac 301 caaaacggaa acgaggtgaa aagggagaag tggtggaaac tgttgaagat gttattgttc 361 ggaaattgac tgctgagcga gttgaagaac taaagaaagt gataaaggaa acccaggaga 421 gatatagacg gctaaagaga gatgcagaac taattcaagc tggacacatg gacagcagac 481 tggatgagct ttgcaatgac attgcaacga aaaagaaatt ggaagaagag gaggctgaag 541 taaagaggaa ggctacagat gctgcatacc aggctcgtca agcagtaaaa acaccccccc 601 ggaggttacc cactgtgatg gttcgctctc ctatagattc tgcctcccca ggaggtgatt 661 atccacttgg ggacttgact ccaaccacta tggaagaggc tacctctggg gtaacccccg 721 ggactttgcc gagtacccca gtcacctcgt ttcctgggat tcctgacacc cttcctccag 781 gctctgcacc cttagaagcc cccatgaccc cagtaacaga tgattcaccc cagaaaaaga 841 tgcttggaca gaaagcaact ccacccccct cccctctgct gtcagagctc ttgaagaagg 901 gcagcctcct gcctactagc cccagactgg tcaatgagag tgaaatggct gtggcttctg 961 gccacctgaa cagtacaggt gtcctcctgg aggtaggcgg ggtccttccc atgatacatg 1021 gtggggagat acagcaaaca cccaatactg ttgcagcctc ccctgctgca tcaggtgctc 1081 ccactctttc ccggctttta gaagctggtc ctacacagtt caccacacct cttgcttcct 1141 tcactactgt tgccagtgag cctccagtta aacttgtgcc accccctgta gagtctgtgt 1201 cccaagctac cattgtcatg atgcctgcgc tgccagcacc atcctctgct ccggctgtct 1261 ccactactga aagtgtagct ccagtgagtc aacccgacaa ctgtgttccc atggaggctg 1321 tgggggatcc acatactgtg actgtttcca tggacagcag tgaaatatcc atgatcatca 1381 attctatcaa agaagagtgt tttcgatcag gggtagcaga ggctcctgtt ggatcaaagg 1441 ctcccagcat agatgggaag gaagaattag atctggctga gaagatggat attgctgtgt 1501 cttacacagg tgaagagctg gattttgaga ctgttggaga catcattgcc atcattgagg 1561 acaaggtaga tgatcatcct gaagtgctgg atgtggcagc agtggaagca gcactgtcat 1621 tttgtgaaga aaatgatgat cctcagtccc tgcctggccc ctgggagcat cctatccagc 1681 aggagcggga caagccagta cctctccctg caccagaaat gacggtcaag caagagagac 1741 tggactttga ggaaacggaa aacaagggaa tacatgaact ggtggacatc agggagccca 1801 gtgcagagat caaggtggaa cctgcagaac cagagccagt catttcagga gccgaaatag 1861 tagctggagt tgttccagcc acaagtatgg agccaccaga actcaggagt caggacttag 1921 atgaggaact gggaagtact gcagctggag agattgttga agcagatgtt gccattggga 1981 aaggcgatga gactccactt acaaatgtga agacagaggc atcccctgaa agcatgttgt 2041 ctccatcaca tggctcaaat cccattgaag atcctttaga ggcagagact cagcacaagt 2101 ttgaaatgtc agactcattg aaagaagaat cagggactat ttttggaagc cagataaagg 2161 atgccccagg tgaggatgag gaggaagatg gtgtcagtga agcggccagc ctagaggagc 2221 ctaaggaaga ggatcaagga gaaggctact tgtcagaaat ggataatgaa cctcctgtga 2281 gcgagagtga tgatggcttc agcatacaca atgctacact gcagtcacac acactggcag 2341 actccatccc cagcagccct gcttcttcac agttctctgt ctgtagtgag gatcaggaag 2401 ctattcaggc acagaaaatt tggaagaaag ccatcatgct tgtatggaga gctgcagcta 2461 atcataggta tgccaatgtc ttcctgcagc ctgttacaga tgacatagca cctggctacc 2521 acagcattgt gcagaggcct atggatttgt caactattaa gaaaaacata gaaaatggac 2581 tgatccgaag cacagctgaa tttcagcgtg acattatgct gatgtttcag aatgctgtaa 2641 tgtacaatag ctcagaccat gatgtctatc acatggcagt ggagatgcag cgagatgtct 2701 tggaacagat ccagcaattc ttGGCCACGC AGTTGATTAT GCAAACATCC GAGTCTGGGA 2761 TCAGTGCTAA AAGTCTTCGA GGGAGAGATT CTACCCGCAA ACAGGATGCT TCAGAGAAGG 2821 ACAGTGTCCC AATGGGCTCT CCTGCCTTCC TTCTCTCTCT CTTTGATGGA GGAACCAGGG 2881 GACGCCGCTG TGCCATTGAA GCAGATATGA AGATGAAAAA GTGAGACCCA GCTTTCTTGT 2941 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3001 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3061 GAAAGGACGA ATTGGCGTGT GCGCATAAAG AAGGACGCGT TAAGTCgaca atcaacctct 3121 ggattacaaa atttgtgaaa gatt