Construct: ORF ccsbBroad304_11567

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016551.1_s304c1, BRDN0000399203
Derived from:
ccsbBroadEn_11567
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BRD8 (10902)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11567
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10902 BRD8 bromodomain containing 8 NM_006696.4 96.7% 96.7% 1_93del
2 human 10902 BRD8 bromodomain containing 8 NM_001300961.2 90.5% 89.2% 1_40del;63_67delGGTAA;593_594ins219
3 human 10902 BRD8 bromodomain containing 8 NM_001300962.2 88.2% 88.3% 1_40del;63_64ins70;518_519ins219
4 human 10902 BRD8 bromodomain containing 8 NM_001164326.1 87.8% 87.8% 1_93del;1003_1004ins210;2541_2542ins45
5 human 10902 BRD8 bromodomain containing 8 NM_001300966.2 82.2% 81.5% (many diffs)
6 human 10902 BRD8 bromodomain containing 8 NM_139199.2 64.1% 63.2% (many diffs)
7 mouse 78656 Brd8 bromodomain containing 8 NM_030147.3 88.4% 91.7% (many diffs)
8 mouse 78656 Brd8 bromodomain containing 8 XM_006526355.3 86.9% 90.2% (many diffs)
9 mouse 78656 Brd8 bromodomain containing 8 XM_006526354.3 86.4% 89% (many diffs)
10 mouse 78656 Brd8 bromodomain containing 8 XM_011247042.1 82.2% 84.3% (many diffs)
11 mouse 78656 Brd8 bromodomain containing 8 NM_001289607.1 81.5% 84.8% (many diffs)
12 mouse 78656 Brd8 bromodomain containing 8 NM_001289606.1 80.8% 84.2% (many diffs)
13 mouse 78656 Brd8 bromodomain containing 8 XM_006526358.3 80% 83.2% (many diffs)
14 mouse 78656 Brd8 bromodomain containing 8 XM_011247038.2 79.6% 82.2% (many diffs)
15 mouse 78656 Brd8 bromodomain containing 8 XM_011247039.2 78.9% 81.6% (many diffs)
16 mouse 78656 Brd8 bromodomain containing 8 XR_001782404.1 72.4% (many diffs)
17 mouse 78656 Brd8 bromodomain containing 8 XM_011247040.2 72.1% 74.7% (many diffs)
18 mouse 78656 Brd8 bromodomain containing 8 XR_001782405.1 68.4% (many diffs)
19 mouse 78656 Brd8 bromodomain containing 8 XM_006526359.3 65% 65.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2826
ORF length:
2760
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag aagtggcgat caaaattggg tatcagttag cagagcaatc aagccctttg
121cagaacctgg ccgccctcca gactggttct ctcaaaaaca ttgtgcttcc cagtactcgg
181agcttttaga gaccactgag acaccaaaac ggaaacgagg tgaaaaggga gaagtggtgg
241aaactgttga agatgttatt gttcggaaat tgactgctga gcgagttgaa gaactaaaga
301aagtgataaa ggaaacccag gagagatata gacggctaaa gagagatgca gaactaattc
361aagctggaca catggacagc agactggatg agctttgcaa tgacattgca acgaaaaaga
421aattggaaga agaggaggct gaagtaaaga ggaaggctac agatgctgca taccaggctc
481gtcaagcagt aaaaacaccc ccccggaggt tacccactgt gatggttcgc tctcctatag
541attctgcctc cccaggaggt gattatccac ttggggactt gactccaacc actatggaag
601aggctacctc tggggtaacc cccgggactt tgccgagtac cccagtcacc tcgtttcctg
661ggattcctga cacccttcct ccaggctctg cacccttaga agcccccatg accccagtaa
721cagatgattc accccagaaa aagatgcttg gacagaaagc aactccaccc ccctcccctc
781tgctgtcaga gctcttgaag aagggcagcc tcctgcctac tagccccaga ctggtcaatg
841agagtgaaat ggctgtggct tctggccacc tgaacagtac aggtgtcctc ctggaggtag
901gcggggtcct tcccatgata catggtgggg agatacagca aacacccaat actgttgcag
961cctcccctgc tgcatcaggt gctcccactc tttcccggct tttagaagct ggtcctacac
1021agttcaccac acctcttgct tccttcacta ctgttgccag tgagcctcca gttaaacttg
1081tgccaccccc tgtagagtct gtgtcccaag ctaccattgt catgatgcct gcgctgccag
1141caccatcctc tgctccggct gtctccacta ctgaaagtgt agctccagtg agtcaacccg
1201acaactgtgt tcccatggag gctgtggggg atccacatac tgtgactgtt tccatggaca
1261gcagtgaaat atccatgatc atcaattcta tcaaagaaga gtgttttcga tcaggggtag
1321cagaggctcc tgttggatca aaggctccca gcatagatgg gaaggaagaa ttagatctgg
1381ctgagaagat ggatattgct gtgtcttaca caggtgaaga gctggatttt gagactgttg
1441gagacatcat tgccatcatt gaggacaagg tagatgatca tcctgaagtg ctggatgtgg
1501cagcagtgga agcagcactg tcattttgtg aagaaaatga tgatcctcag tccctgcctg
1561gcccctggga gcatcctatc cagcaggagc gggacaagcc agtacctctc cctgcaccag
1621aaatgacggt caagcaagag agactggact ttgaggaaac ggaaaacaag ggaatacatg
1681aactggtgga catcagggag cccagtgcag agatcaaggt ggaacctgca gaaccagagc
1741cagtcatttc aggagccgaa atagtagctg gagttgttcc agccacaagt atggagccac
1801cagaactcag gagtcaggac ttagatgagg aactgggaag tactgcagct ggagagattg
1861ttgaagcaga tgttgccatt gggaaaggcg atgagactcc acttacaaat gtgaagacag
1921aggcatcccc tgaaagcatg ttgtctccat cacatggctc aaatcccatt gaagatcctt
1981tagaggcaga gactcagcac aagtttgaaa tgtcagactc attgaaagaa gaatcaggga
2041ctatttttgg aagccagata aaggatgccc caggtgagga tgaggaggaa gatggtgtca
2101gtgaagcggc cagcctagag gagcctaagg aagaggatca aggagaaggc tacttgtcag
2161aaatggataa tgaacctcct gtgagcgaga gtgatgatgg cttcagcata cacaatgcta
2221cactgcagtc acacacactg gcagactcca tccccagcag ccctgcttct tcacagttct
2281ctgtctgtag tgaggatcag gaagctattc aggcacagaa aatttggaag aaagccatca
2341tgcttgtatg gagagctgca gctaatcata ggtatgccaa tgtcttcctg cagcctgtta
2401cagatgacat agcacctggc taccacagca ttgtgcagag gcctatggat ttgtcaacta
2461ttaagaaaaa catagaaaat ggactgatcc gaagcacagc tgaatttcag cgtgacatta
2521tgctgatgtt tcagaatgct gtaatgtaca atagctcaga ccatgatgtc tatcacatgg
2581cagtggagat gcagcgagat gtcttggaac agatccagca attcttggcc acgcagttga
2641ttatgcaaac atccgagtct gggatcagtg ctaaaagtct tcgagggaga gattctaccc
2701gcaaacagga tgcttcagag aaggacagtg tcccaatggg ctctcctgcc ttccttctct
2761ctctctttga tggaggaacc aggggacgcc gctgtgccat tgaagcagat atgaagatga
2821aaaagtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2881gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2941acctctggat ta

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