Construct: ORF ccsbBroad304_11567
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016551.1_s304c1, BRDN0000399203
- Derived from:
- ccsbBroadEn_11567
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BRD8 (10902)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11567
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10902 | BRD8 | bromodomain containing 8 | NM_006696.4 | 96.7% | 96.7% | 1_93del |
| 2 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300961.2 | 90.5% | 89.2% | 1_40del;63_67delGGTAA;593_594ins219 |
| 3 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300962.2 | 88.2% | 88.3% | 1_40del;63_64ins70;518_519ins219 |
| 4 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001164326.1 | 87.8% | 87.8% | 1_93del;1003_1004ins210;2541_2542ins45 |
| 5 | human | 10902 | BRD8 | bromodomain containing 8 | NM_001300966.2 | 82.2% | 81.5% | (many diffs) |
| 6 | human | 10902 | BRD8 | bromodomain containing 8 | NM_139199.2 | 64.1% | 63.2% | (many diffs) |
| 7 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_030147.3 | 88.4% | 91.7% | (many diffs) |
| 8 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526355.3 | 86.9% | 90.2% | (many diffs) |
| 9 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526354.3 | 86.4% | 89% | (many diffs) |
| 10 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247042.1 | 82.2% | 84.3% | (many diffs) |
| 11 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_001289607.1 | 81.5% | 84.8% | (many diffs) |
| 12 | mouse | 78656 | Brd8 | bromodomain containing 8 | NM_001289606.1 | 80.8% | 84.2% | (many diffs) |
| 13 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526358.3 | 80% | 83.2% | (many diffs) |
| 14 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247038.2 | 79.6% | 82.2% | (many diffs) |
| 15 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247039.2 | 78.9% | 81.6% | (many diffs) |
| 16 | mouse | 78656 | Brd8 | bromodomain containing 8 | XR_001782404.1 | 72.4% | (many diffs) | |
| 17 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_011247040.2 | 72.1% | 74.7% | (many diffs) |
| 18 | mouse | 78656 | Brd8 | bromodomain containing 8 | XR_001782405.1 | 68.4% | (many diffs) | |
| 19 | mouse | 78656 | Brd8 | bromodomain containing 8 | XM_006526359.3 | 65% | 65.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2826
- ORF length:
- 2760
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag aagtggcgat caaaattggg tatcagttag cagagcaatc aagccctttg 121 cagaacctgg ccgccctcca gactggttct ctcaaaaaca ttgtgcttcc cagtactcgg 181 agcttttaga gaccactgag acaccaaaac ggaaacgagg tgaaaaggga gaagtggtgg 241 aaactgttga agatgttatt gttcggaaat tgactgctga gcgagttgaa gaactaaaga 301 aagtgataaa ggaaacccag gagagatata gacggctaaa gagagatgca gaactaattc 361 aagctggaca catggacagc agactggatg agctttgcaa tgacattgca acgaaaaaga 421 aattggaaga agaggaggct gaagtaaaga ggaaggctac agatgctgca taccaggctc 481 gtcaagcagt aaaaacaccc ccccggaggt tacccactgt gatggttcgc tctcctatag 541 attctgcctc cccaggaggt gattatccac ttggggactt gactccaacc actatggaag 601 aggctacctc tggggtaacc cccgggactt tgccgagtac cccagtcacc tcgtttcctg 661 ggattcctga cacccttcct ccaggctctg cacccttaga agcccccatg accccagtaa 721 cagatgattc accccagaaa aagatgcttg gacagaaagc aactccaccc ccctcccctc 781 tgctgtcaga gctcttgaag aagggcagcc tcctgcctac tagccccaga ctggtcaatg 841 agagtgaaat ggctgtggct tctggccacc tgaacagtac aggtgtcctc ctggaggtag 901 gcggggtcct tcccatgata catggtgggg agatacagca aacacccaat actgttgcag 961 cctcccctgc tgcatcaggt gctcccactc tttcccggct tttagaagct ggtcctacac 1021 agttcaccac acctcttgct tccttcacta ctgttgccag tgagcctcca gttaaacttg 1081 tgccaccccc tgtagagtct gtgtcccaag ctaccattgt catgatgcct gcgctgccag 1141 caccatcctc tgctccggct gtctccacta ctgaaagtgt agctccagtg agtcaacccg 1201 acaactgtgt tcccatggag gctgtggggg atccacatac tgtgactgtt tccatggaca 1261 gcagtgaaat atccatgatc atcaattcta tcaaagaaga gtgttttcga tcaggggtag 1321 cagaggctcc tgttggatca aaggctccca gcatagatgg gaaggaagaa ttagatctgg 1381 ctgagaagat ggatattgct gtgtcttaca caggtgaaga gctggatttt gagactgttg 1441 gagacatcat tgccatcatt gaggacaagg tagatgatca tcctgaagtg ctggatgtgg 1501 cagcagtgga agcagcactg tcattttgtg aagaaaatga tgatcctcag tccctgcctg 1561 gcccctggga gcatcctatc cagcaggagc gggacaagcc agtacctctc cctgcaccag 1621 aaatgacggt caagcaagag agactggact ttgaggaaac ggaaaacaag ggaatacatg 1681 aactggtgga catcagggag cccagtgcag agatcaaggt ggaacctgca gaaccagagc 1741 cagtcatttc aggagccgaa atagtagctg gagttgttcc agccacaagt atggagccac 1801 cagaactcag gagtcaggac ttagatgagg aactgggaag tactgcagct ggagagattg 1861 ttgaagcaga tgttgccatt gggaaaggcg atgagactcc acttacaaat gtgaagacag 1921 aggcatcccc tgaaagcatg ttgtctccat cacatggctc aaatcccatt gaagatcctt 1981 tagaggcaga gactcagcac aagtttgaaa tgtcagactc attgaaagaa gaatcaggga 2041 ctatttttgg aagccagata aaggatgccc caggtgagga tgaggaggaa gatggtgtca 2101 gtgaagcggc cagcctagag gagcctaagg aagaggatca aggagaaggc tacttgtcag 2161 aaatggataa tgaacctcct gtgagcgaga gtgatgatgg cttcagcata cacaatgcta 2221 cactgcagtc acacacactg gcagactcca tccccagcag ccctgcttct tcacagttct 2281 ctgtctgtag tgaggatcag gaagctattc aggcacagaa aatttggaag aaagccatca 2341 tgcttgtatg gagagctgca gctaatcata ggtatgccaa tgtcttcctg cagcctgtta 2401 cagatgacat agcacctggc taccacagca ttgtgcagag gcctatggat ttgtcaacta 2461 ttaagaaaaa catagaaaat ggactgatcc gaagcacagc tgaatttcag cgtgacatta 2521 tgctgatgtt tcagaatgct gtaatgtaca atagctcaga ccatgatgtc tatcacatgg 2581 cagtggagat gcagcgagat gtcttggaac agatccagca attcttggcc acgcagttga 2641 ttatgcaaac atccgagtct gggatcagtg ctaaaagtct tcgagggaga gattctaccc 2701 gcaaacagga tgcttcagag aaggacagtg tcccaatggg ctctcctgcc ttccttctct 2761 ctctctttga tggaggaacc aggggacgcc gctgtgccat tgaagcagat atgaagatga 2821 aaaagtgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2881 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2941 acctctggat ta