Construct: ORF TRCN0000473658

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015822.3_s317c1
Derived from:
ccsbBroadEn_14840
DNA Barcode:
TATATATCTACAATCTCAGAACCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SGK1 (6446)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473658
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6446 SGK1 serum/glucocorticoid regula... NM_005627.4 99.9% 99.7% 1290C>A
2 human 6446 SGK1 serum/glucocorticoid regula... NM_001143678.2 93.7% 90.6% (many diffs)
3 human 6446 SGK1 serum/glucocorticoid regula... NM_001143677.2 91.1% 87% (many diffs)
4 human 6446 SGK1 serum/glucocorticoid regula... NM_001291995.2 89.7% 89.5% 413_414ins132;1158C>A
5 human 6446 SGK1 serum/glucocorticoid regula... NM_001143676.1 80.3% 75% (many diffs)
6 human 6446 SGK1 serum/glucocorticoid regula... XM_011536071.1 80.3% 75% (many diffs)
7 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_011361.3 88.6% 96.2% (many diffs)
8 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_001161849.2 85.5% 91.6% (many diffs)
9 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_001161850.2 83.8% 88% (many diffs)
10 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_001161847.2 83.5% 90.7% (many diffs)
11 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_001161848.2 83.5% 90.7% (many diffs)
12 mouse 20393 Sgk1 serum/glucocorticoid regula... NM_001161845.2 71.9% 72.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1359
ORF length:
1293
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgac ggtgaaaact gaggctgcta agggcaccct cacttactcc aggatgaggg
121gcatggtggc aattctcatc gctttcatga agcagaggag gatgggtctg aacgacttta
181ttcagaagat tgccaataac tcctatgcat gcaaacaccc tgaagttcag tccatcttga
241agatctccca acctcaggag cctgagctta tgaatgccaa cccttctcct ccaccaagtc
301cttctcagca aatcaacctt ggcccgtcgt ccaatcctca tgctaaacca tctgactttc
361acttcttgaa agtgatcgga aagggcagtt ttggaaaggt tcttctagca agacacaagg
421cagaagaagt gttctatgca gtcaaagttt tacagaagaa agcaatcctg aaaaagaaag
481aggagaagca tattatgtcg gagcggaatg ttctgttgaa gaatgtgaag caccctttcc
541tggtgggcct tcacttctct ttccagactg ctgacaaatt gtactttgtc ctagactaca
601ttaatggtgg agagttgttc taccatctcc agagggaacg ctgcttcctg gaaccacggg
661ctcgtttcta tgctgctgaa atagccagtg ccttgggcta cctgcattca ctgaacatcg
721tttatagaga cttaaaacca gagaatattt tgctagattc acagggacac attgtcctta
781ctgacttcgg actctgcaag gagaacattg aacacaacag cacaacatcc accttctgtg
841gcacgccgga gtatctcgca cctgaggtgc ttcataagca gccttatGAC AGGACTGTGG
901ACTGGTGGTG CCTGGGAGCT GTCTTGTATG AGATGCTGTA TGGCCTGCCG CCTTTTTATA
961GCCGAAACAC AGCTGAAATG TACGACAACA TTCTGAACAA GCCTCTCCAG CTGAAACCAA
1021ATATTACAAA TTCCGCAAGA CACCTCCTGG AGGGCCTCCT GCAGAAGGAC AGGACAAAGC
1081GGCTCGGGGC CAAGGATGAC TTCATGGAGA TTAAGAGTCA TGTCTTCTTC TCCTTAATTA
1141ACTGGGATGA TCTCATTAAT AAGAAGATTA CTCCCCCTTT TAACCCAAAT GTGAGTGGGC
1201CCAACGACCT ACGGCACTTT GACCCCGAGT TTACCGAAGA GCCTGTCCCC AACTCCATTG
1261GCAAGTCCCC TGACAGCGTC CTCGTCACAG CCAGCGTCAA GGAAGCTGCC GAGGCTTTCC
1321TAGGCTTTTC CTATGCGCCT CCCACGGACT CTTTACTCTG CCCAACTTTC TTGTACAAAG
1381TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
1441GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
1501ACGATATATA TCTACAATCT CAGAACCCAC GCGTTAAGTC gacaatcaac ctctggatta
1561caaaatttgt gaaagatt

Download FASTA (ORF) (Full)