Construct: ORF TRCN0000491429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020539.2_s317c1
- DNA Barcode:
- CTCCCGTCGCCGTGCATTGGACCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CLK4 (57396)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57396 | CLK4 | CDC like kinase 4 | NM_020666.3 | 100% | 100% | |
| 2 | mouse | 12750 | Clk4 | CDC like kinase 4 | NM_007714.6 | 90.6% | 97% | (many diffs) |
| 3 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532112.3 | 83.9% | 86.2% | (many diffs) |
| 4 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532113.3 | 83.9% | 86.2% | (many diffs) |
| 5 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_011248709.2 | 83.9% | 86.2% | (many diffs) |
| 6 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532111.3 | 82.9% | 82.2% | (many diffs) |
| 7 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_011248708.2 | 82.4% | 83.5% | (many diffs) |
| 8 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_017314243.1 | 78.9% | 80.9% | (many diffs) |
| 9 | mouse | 12750 | Clk4 | CDC like kinase 4 | NM_001304744.1 | 56.6% | 61.5% | (many diffs) |
| 10 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532114.3 | 56.6% | 61.5% | (many diffs) |
| 11 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532115.3 | 56.6% | 61.5% | (many diffs) |
| 12 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532116.2 | 56.6% | 61.5% | (many diffs) |
| 13 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_006532118.2 | 56.6% | 61.5% | (many diffs) |
| 14 | mouse | 12750 | Clk4 | CDC like kinase 4 | XM_017314244.1 | 56.6% | 61.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1515
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcggcat tccaaaagaa ctcactgtcc tgattgggat agcagagaaa 121 gctggggaca tgaaagctat cgtggaagtc acaagcggaa gaggagatct catagtagca 181 cacaagagaa caggcattgt aaaccacatc accagtttaa agaatctgat tgtcattatt 241 tagaagcaag gtccttgaat gagcgagatt atcgggaccg gagatacgtt gacgaataca 301 ggaatgacta ctgtgaagga tatgttccta gacattatca cagagacatt gaaagcgggt 361 atcgaatcca ctgcagtaaa tcttcagtcc gcagcaggag aagcagtcct aaaaggaagc 421 gcaatagaca ctgttcaagt catcagtcac gttcgaagag ccaccgaagg aaaagatcca 481 ggagtataga ggatgatgag gagggtcacc tgatctgtca aagtggagac gttctaagag 541 caagatatga aatcgtggac actttgggtg aaggagcctt tggcaaagtt gtagagtgca 601 ttgatcatgg catggatggc atgcatgtag cagtgaaaat cgtaaaaaat gtaggccgtt 661 accgtgaagc agctcgttca gaaatccaag tattagagca cttaaatagt actgatccca 721 atagtgtctt ccgatgtgtc cagatgctag aatggtttga tcatcatggt catgtttgta 781 ttgtgtttga actactggga cttagtactt acgatttcat taaagaaaac agctttctgc 841 catttcaaat tgaccacatc aggcagatgg cgtatcagat ctgccagtca ataaattttt 901 tacatcataa taaattaacc catacagatc tgaagcctga aaatattttg tttgtgaagt 961 ctgactatgt agtcaaatat aattctaaaa tgaaacgtga tgaacgcaca ctgaaaaaca 1021 cagatatcaa agttgttgac tttggaagtg caacgtatga tgatgaacat cacagtactt 1081 tggtgtctac ccggcactac agagctcccg aggtcatttt ggctttaggt tggtcTCAGC 1141 CTTGTGATGT TTGGAGCATA GGTTGCATTC TTATTGAATA TTACCTTGGT TTCACAGTCT 1201 TTCAGACTCA TGATAGTAAA GAGCACCTGG CAATGATGGA ACGAATATTA GGACCCATAC 1261 CACAACACAT GATTCAGAAA ACAAGAAAAC GCAAGTATTT TCACCATAAC CAGCTAGATT 1321 GGGATGAACA CAGTTCTGCT GGTAGATATG TTAGGAGACG CTGCAAACCG TTGAAGGAAT 1381 TTATGCTTTG TCATGATGAA GAACATGAGA AACTGTTTGA CCTGGTTCGA AGAATGTTAG 1441 AATATGATCC AACTCAAAGA ATTACCTTGG ATGAAGCATT GCAGCATCCT TTCTTTGACT 1501 TATTAAAAAA GAAATGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1561 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1621 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCCCGT CGCCGTGCAT 1681 TGGACCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt