Transcript: Mouse NM_033574.3

Mus musculus protocadherin gamma subfamily B, 1 (Pcdhgb1), mRNA.

Source:
NCBI, updated 2015-07-31
Taxon:
Mus musculus (mouse)
Gene:
Pcdhgb1 (93699)
Length:
4498
CDS:
1..2757

Additional Resources:

NCBI RefSeq record:
NM_033574.3
NBCI Gene record:
Pcdhgb1 (93699)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_033574.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000094320 CCTCATTAAAGTTCGAGACAA pLKO.1 1065 CDS 100% 4.950 6.930 N Pcdhgb1 n/a
2 TRCN0000094321 GCAGAGGATTATTTCAGCGTT pLKO.1 175 CDS 100% 2.640 2.112 N Pcdhgb1 n/a
3 TRCN0000094323 CAACTAATGGTACATTGGATT pLKO.1 869 CDS 100% 4.950 3.465 N Pcdhgb1 n/a
4 TRCN0000056521 CGAAGGAAAGTCCTGATGGAA pLKO.1 509 CDS 100% 3.000 2.100 N PCDHGB1 n/a
5 TRCN0000094322 GCCATCCAAGACATTAACGAT pLKO.1 328 CDS 100% 3.000 2.100 N Pcdhgb1 n/a
6 TRCN0000094239 CCCTGTACTGACTTCTCTATA pLKO.1 4348 3UTR 100% 13.200 6.600 Y Pcdhga10 n/a
7 TRCN0000094839 CCTGTACTGACTTCTCTATAA pLKO.1 4349 3UTR 100% 13.200 6.600 Y Pcdhga11 n/a
8 TRCN0000094814 GACGACTTCTAAGTGAGTTTA pLKO.1 3787 3UTR 100% 13.200 6.600 Y Pcdhgb6 n/a
9 TRCN0000094199 GCTGGTGTAGAATAGCCAATA pLKO.1 4186 3UTR 100% 10.800 5.400 Y Pcdhgb4 n/a
10 TRCN0000094769 CCTATTCAATCAGTGATTGTA pLKO.1 2979 3UTR 100% 5.625 2.813 Y Pcdhgc4 n/a
11 TRCN0000094989 CCTTACCAGGTGCCATTTCTT pLKO.1 4030 3UTR 100% 5.625 2.813 Y Pcdhga8 n/a
12 TRCN0000094369 CGGTAGGGAGAGTATTACAAT pLKO.1 3239 3UTR 100% 5.625 2.813 Y Pcdhga12 n/a
13 TRCN0000094684 CAATGGATTTAAGCTGACATT pLKO.1 3754 3UTR 100% 4.950 2.475 Y Pcdhga5 n/a
14 TRCN0000094289 CCAATGGATTTAAGCTGACAT pLKO.1 3753 3UTR 100% 4.950 2.475 Y Pcdhga9 n/a
15 TRCN0000094319 CCTAGCCCTTACAGTAGTGTA pLKO.1 4148 3UTR 100% 4.950 2.475 Y Pcdhgb1 n/a
16 TRCN0000094649 CCTCAAAGAAGAGACTCCTTT pLKO.1 3509 3UTR 100% 4.950 2.475 Y Pcdhga1 n/a
17 TRCN0000094049 CCTCTCCTTCAGTTCAGCTTT pLKO.1 3267 3UTR 100% 4.950 2.475 Y Pcdhgb5 n/a
18 TRCN0000095019 CCTTACAGTAGTGTAGAAGAT pLKO.1 4154 3UTR 100% 4.950 2.475 Y Pcdhga4 n/a
19 TRCN0000094104 CGACGACTTCTAAGTGAGTTT pLKO.1 3786 3UTR 100% 4.950 2.475 Y Pcdhga2 n/a
20 TRCN0000094504 CGTCTGTCCTTTGAATCTCAA pLKO.1 4269 3UTR 100% 4.950 2.475 Y Pcdhga3 n/a
21 TRCN0000094724 GCCTCCTTGTGCATAGAACTT pLKO.1 4120 3UTR 100% 4.950 2.475 Y Pcdhgb7 n/a
22 TRCN0000094919 GTGGTAAACTAAAGGGAAGTT pLKO.1 3552 3UTR 100% 4.950 2.475 Y Pcdhgb8 n/a
23 TRCN0000094374 CGCTGGTGTAGAATAGCCAAT pLKO.1 4185 3UTR 100% 4.050 2.025 Y Pcdhgc3 n/a
24 TRCN0000334198 CGCTGGTGTAGAATAGCCAAT pLKO_005 4185 3UTR 100% 4.050 2.025 Y Pcdhgc3 n/a
25 TRCN0000094509 CCTCTGGTACTGATGATGCTT pLKO.1 3375 3UTR 100% 3.000 1.500 Y Pcdhgb2 n/a
26 TRCN0000095024 GCCTGTTAATAGGGTCTTGCA pLKO.1 3613 3UTR 100% 2.640 1.320 Y Pcdhga6 n/a
27 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3036 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033574.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01151 pDONR223 100% 12.9% 13.4% None (many diffs) n/a
2 ccsbBroad304_01151 pLX_304 0% 12.9% 13.4% V5 (many diffs) n/a
3 TRCN0000466539 CATGGTTCGGTAATGAAAGTTCCA pLX_317 36.4% 12.9% 13.4% V5 (many diffs) n/a
4 ccsbBroadEn_11019 pDONR223 100% 5.8% 6.5% None (many diffs) n/a
5 ccsbBroad304_11019 pLX_304 0% 5.8% 6.5% V5 (many diffs) n/a
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