Transcript: Human NR_146935.1

Homo sapiens thiamin pyrophosphokinase 1 (TPK1), transcript variant 17, non-coding RNA.

Source:
NCBI, updated 2019-04-02
Taxon:
Homo sapiens (human)
Gene:
TPK1 (27010)
Length:
2372
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_146935.1
NBCI Gene record:
TPK1 (27010)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_146935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000038006 GACTTTACTAAGTGCCTTAAA pLKO.1 481 3UTR 100% 13.200 10.560 N TPK1 n/a
2 TRCN0000197025 GCAGTCATAGGGCTGATAATA pLKO.1 1803 3UTR 100% 15.000 10.500 N TPK1 n/a
3 TRCN0000195020 CAAGAATCATTGACCTAATTG pLKO.1 1321 3UTR 100% 13.200 9.240 N TPK1 n/a
4 TRCN0000038004 CCTGCTTTCCACTGGGAATTT pLKO.1 283 3UTR 100% 13.200 9.240 N TPK1 n/a
5 TRCN0000219786 GTACTGCCTTGTAATTCTTAA pLKO.1 307 3UTR 100% 13.200 9.240 N TPK1 n/a
6 TRCN0000219787 GTGATTGGTGTGGCCTTATTC pLKO.1 575 3UTR 100% 13.200 9.240 N TPK1 n/a
7 TRCN0000038007 CAGAGAATACTATGCTACTAA pLKO.1 420 3UTR 100% 5.625 3.938 N TPK1 n/a
8 TRCN0000038008 CATTGGTCAGTACTTCCAATA pLKO.1 674 3UTR 100% 1.080 0.756 N TPK1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_146935.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491741 TACGCAACGCCATGAATTACAAAA pLX_317 83.7% 14.4% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14113 pDONR223 100% 14.3% None (many diffs) n/a
3 ccsbBroad304_14113 pLX_304 0% 14.3% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000471369 ATATTAAGTTAGTCCCACAAACCT pLX_317 100% 14.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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