Transcript: Mouse NM_008927.3

Mus musculus mitogen-activated protein kinase kinase 1 (Map2k1), mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Map2k1 (26395)
Length:
2387
CDS:
314..1495

Additional Resources:

NCBI RefSeq record:
NM_008927.3
NBCI Gene record:
Map2k1 (26395)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_008927.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000025214 GCCATCCAACATTCTAGTGAA pLKO.1 889 CDS 100% 4.950 6.930 N Map2k1 n/a
2 TRCN0000418158 CAAGCCATCTGGCCTGGTTAT pLKO_005 574 CDS 100% 10.800 7.560 N Map2k1 n/a
3 TRCN0000431703 CCTCTCCCATGCTTGTCTATG pLKO_005 1556 3UTR 100% 10.800 7.560 N Map2k1 n/a
4 TRCN0000415114 TGCTCCACCATTGGGCTTAAC pLKO_005 1439 CDS 100% 10.800 7.560 N Map2k1 n/a
5 TRCN0000199961 CTGGAGATCAAACCCGCAATC pLKO.1 614 CDS 100% 6.000 4.200 N MAP2K1 n/a
6 TRCN0000025217 CCTTGGATCAAGTTCTGAAGA pLKO.1 762 CDS 100% 4.950 3.465 N Map2k1 n/a
7 TRCN0000025218 GCAGCTCATGGTACATGCTTT pLKO.1 1372 CDS 100% 4.950 3.465 N Map2k1 n/a
8 TRCN0000025216 CCTGGAGATCAAACCCGCAAT pLKO.1 613 CDS 100% 4.050 2.835 N Map2k1 n/a
9 TRCN0000412246 GAACGTGTACGTCATGGTATT pLKO_005 1965 3UTR 100% 10.800 6.480 N Map2k1 n/a
10 TRCN0000025215 TGGAGTATTCAGTCTGGAGTT pLKO.1 1294 CDS 100% 4.050 2.430 N Map2k1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_008927.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroad304_14807 pLX_304 50.7% 90.5% 36.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14807 pDONR223 100% 90.5% 36.3% None (many diffs) n/a
3 TRCN0000492332 GTGTTATTTGGCCTAAGAGCTCAC pLX_317 33.1% 90.5% 98.7% V5 (many diffs) n/a
4 ccsbBroadEn_16176 pDONR223 0% 90.3% 98.4% None (many diffs) n/a
5 ccsbBroad304_16176 pLX_304 0% 90.3% 98.4% V5 (many diffs) n/a
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