Transcript: Human XM_017009628.1

PREDICTED: Homo sapiens RIO kinase 2 (RIOK2), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RIOK2 (55781)
Length:
3588
CDS:
297..1394

Additional Resources:

NCBI RefSeq record:
XM_017009628.1
NBCI Gene record:
RIOK2 (55781)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017009628.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194682 CTAATTGCCTTGTCGTCATTA pLKO.1 1089 CDS 100% 13.200 18.480 N RIOK2 n/a
2 TRCN0000037507 CGTCGATTGCAGAAAGGAGAA pLKO.1 1290 CDS 100% 4.050 5.670 N RIOK2 n/a
3 TRCN0000197211 GAAACGTTTCAGCTACGAAAG pLKO.1 557 CDS 100% 6.000 4.200 N RIOK2 n/a
4 TRCN0000196684 GAGCTATGAATCAGTATAGAA pLKO.1 1150 CDS 100% 5.625 3.938 N RIOK2 n/a
5 TRCN0000037508 CCTGCATCAGTATATGATGAA pLKO.1 348 CDS 100% 0.495 0.347 N RIOK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017009628.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488945 ACTCTAAGTAGTCCTCTAATTCAA pLX_317 16.6% 66.1% 66.1% V5 (not translated due to prior stop codon) 0_1ins561 n/a
2 ccsbBroadEn_08585 pDONR223 100% 66% 65.9% None 0_1ins561;1075G>C n/a
3 ccsbBroad304_08585 pLX_304 0% 66% 65.9% V5 0_1ins561;1075G>C n/a
4 TRCN0000475896 GTGGTGCAAATAACGCCCAACTGT pLX_317 18% 66% 65.9% V5 0_1ins561;1075G>C n/a
5 ccsbBroadEn_15109 pDONR223 95.8% 65.4% 61.9% None (many diffs) n/a
6 ccsbBroad304_15109 pLX_304 0% 65.4% 61.9% V5 (many diffs) n/a
7 TRCN0000472502 AAGCGTCGCCGTGCCTCTTGTACT pLX_317 25.2% 65.4% 61.9% V5 (many diffs) n/a
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