Transcript: Human XM_005257015.3

PREDICTED: Homo sapiens musashi RNA binding protein 2 (MSI2), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MSI2 (124540)
Length:
6236
CDS:
99..1139

Additional Resources:

NCBI RefSeq record:
XM_005257015.3
NBCI Gene record:
MSI2 (124540)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005257015.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000423713 CTTCGGATCGAGGGTTGACTA pLKO_005 1218 3UTR 100% 4.950 6.930 N MSI2 n/a
2 TRCN0000432136 TTACACCATGGACGCGTTCAT pLKO_005 710 CDS 100% 4.950 6.930 N MSI2 n/a
3 TRCN0000062812 AGATAGCCTTAGAGACTATTT pLKO.1 200 CDS 100% 13.200 9.240 N MSI2 n/a
4 TRCN0000062809 GTGGAAGATGTAAAGCAATAT pLKO.1 465 CDS 100% 13.200 9.240 N MSI2 n/a
5 TRCN0000417428 TGCAATGCTGATGTTTGATAA pLKO_005 512 CDS 100% 13.200 9.240 N MSI2 n/a
6 TRCN0000062808 CCAGCAAGTGTAGATAAAGTA pLKO.1 312 CDS 100% 5.625 3.938 N MSI2 n/a
7 TRCN0000071977 CCACCATGAGTTAGATTCCAA pLKO.1 344 CDS 100% 3.000 2.100 N Msi2 n/a
8 TRCN0000324687 CCACCATGAGTTAGATTCCAA pLKO_005 344 CDS 100% 3.000 2.100 N Msi2 n/a
9 TRCN0000062811 CCCAACTTCGTGGCGACCTAT pLKO.1 756 CDS 100% 1.650 1.155 N MSI2 n/a
10 TRCN0000071976 GCTACAGTGCTCAACCGAATT pLKO.1 898 CDS 100% 0.000 0.000 N Msi2 n/a
11 TRCN0000353948 GCTACAGTGCTCAACCGAATT pLKO_005 898 CDS 100% 0.000 0.000 N Msi2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005257015.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09483 pDONR223 100% 91.1% 89.7% None (many diffs) n/a
2 ccsbBroad304_09483 pLX_304 0% 91.1% 89.7% V5 (many diffs) n/a
3 TRCN0000465338 GATCTCGCCGAGCCAAACTTGCCT pLX_317 29.5% 91.1% 89.7% V5 (many diffs) n/a
4 ccsbBroadEn_13107 pDONR223 100% 42.4% 40.7% None (many diffs) n/a
5 ccsbBroad304_13107 pLX_304 0% 42.4% 40.7% V5 (many diffs) n/a
6 TRCN0000468295 CTGACGCTGTAATCCCGACCACGA pLX_317 77.8% 42.4% 40.7% V5 (many diffs) n/a
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