Transcript: Mouse XM_017318142.1

PREDICTED: Mus musculus leucine rich repeat and fibronectin type III domain containing 4 (Lrfn4), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Lrfn4 (225875)
Length:
2526
CDS:
323..2233

Additional Resources:

NCBI RefSeq record:
XM_017318142.1
NBCI Gene record:
Lrfn4 (225875)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017318142.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418213 AGACCCTCATCTACCGGATTG pLKO_005 1656 CDS 100% 6.000 8.400 N Lrfn4 n/a
2 TRCN0000194418 CGAGGGAACTTTAGAATCTGA pLKO.1 1519 CDS 100% 3.000 4.200 N Lrfn4 n/a
3 TRCN0000176078 GAGGGAACTTTAGAATCTGAA pLKO.1 1520 CDS 100% 4.950 3.960 N Lrfn4 n/a
4 TRCN0000445603 AGAGTGTGGTGTGACAGAAAG pLKO_005 2220 CDS 100% 10.800 7.560 N Lrfn4 n/a
5 TRCN0000222086 CCCAGTGTGGATGTTCCAAAT pLKO.1 1612 CDS 100% 10.800 7.560 N LRFN4 n/a
6 TRCN0000176206 GACCTAACATTGTCTCGAAAT pLKO.1 548 CDS 100% 10.800 7.560 N Lrfn4 n/a
7 TRCN0000435191 CACCTGACTTCCGCAACATGA pLKO_005 516 CDS 100% 4.950 3.465 N Lrfn4 n/a
8 TRCN0000194638 GCATCTCATTCTCAGTGGCAA pLKO.1 691 CDS 100% 2.640 1.584 N Lrfn4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017318142.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04017 pDONR223 100% 87.7% 96.6% None (many diffs) n/a
2 ccsbBroad304_04017 pLX_304 0% 87.7% 96.6% V5 (many diffs) n/a
3 TRCN0000470428 GCGTGCTTCATTTGTAATAATTTT pLX_317 20.9% 87.7% 96.6% V5 (many diffs) n/a
4 ccsbBroadEn_12528 pDONR223 100% 45% 49.5% None (many diffs) n/a
5 ccsbBroad304_12528 pLX_304 0% 45% 49.5% V5 (many diffs) n/a
6 TRCN0000471108 ACGACTATAATCCCTGAATCGCAC pLX_317 46.3% 45% 49.5% V5 (many diffs) n/a
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