Transcript: Human XM_005247461.5

PREDICTED: Homo sapiens muscleblind like splicing regulator 1 (MBNL1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MBNL1 (4154)
Length:
5627
CDS:
954..2213

Additional Resources:

NCBI RefSeq record:
XM_005247461.5
NBCI Gene record:
MBNL1 (4154)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005247461.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000063964 GCCAACCAGATACCCATAATA pLKO.1 2148 CDS 100% 15.000 21.000 N MBNL1 n/a
2 TRCN0000226191 GCCAACCAGATACCCATAATA pLKO_005 2148 CDS 100% 15.000 21.000 N Mbnl1 n/a
3 TRCN0000063967 CCCATAATATCTGCCGAACAT pLKO.1 2160 CDS 100% 4.950 6.930 N MBNL1 n/a
4 TRCN0000102631 CGCAGTCTTTAACACTGGTAT pLKO.1 1976 CDS 100% 4.950 6.930 N Mbnl1 n/a
5 TRCN0000102634 AGCCAACCAGATACCCATAAT pLKO.1 2147 CDS 100% 13.200 10.560 N Mbnl1 n/a
6 TRCN0000419160 ATTTGTGCTGAGGTGATATTC pLKO_005 2437 3UTR 100% 13.200 10.560 N MBNL1 n/a
7 TRCN0000429155 GAGATAAATGGACGCAATAAC pLKO_005 1284 CDS 100% 13.200 10.560 N MBNL1 n/a
8 TRCN0000063963 GCAGTCTTTAACACTGGTATT pLKO.1 1977 CDS 100% 10.800 8.640 N MBNL1 n/a
9 TRCN0000063966 CCAATGTTGGTTACAGGGAAT pLKO.1 1506 CDS 100% 4.050 3.240 N MBNL1 n/a
10 TRCN0000226189 CAGTCTTTAACACTGGTATTT pLKO_005 1978 CDS 100% 13.200 9.240 N Mbnl1 n/a
11 TRCN0000427657 GAGTAAAGGACGAGGTCATTA pLKO_005 2268 3UTR 100% 13.200 9.240 N MBNL1 n/a
12 TRCN0000063965 GCCTGCTTTGATTCATTGAAA pLKO.1 1107 CDS 100% 5.625 3.938 N MBNL1 n/a
13 TRCN0000102630 GCACAGAGTTAGCACTCCATA pLKO.1 4796 3UTR 100% 4.950 3.465 N Mbnl1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005247461.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00980 pDONR223 100% 85.8% 85.8% None 175_267del;899_952del;1107_1108ins36 n/a
2 ccsbBroad304_00980 pLX_304 0% 85.8% 85.8% V5 175_267del;899_952del;1107_1108ins36 n/a
3 TRCN0000467871 TGTGAAGGTACTCGGCAACAGACC pLX_317 30.6% 85.8% 85.8% V5 175_267del;899_952del;1107_1108ins36 n/a
4 ccsbBroadEn_15496 pDONR223 0% 76.6% 76.5% None 1_265del;267_268insG;1107_1108ins36 n/a
5 ccsbBroad304_15496 pLX_304 0% 76.6% 76.5% V5 1_265del;267_268insG;1107_1108ins36 n/a
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