Construct: ORF ccsbBroadEn_15496

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016482.1_s300c1, BRDN0000397177
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MBNL1 (4154)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4154 MBNL1 muscleblind like splicing r... NM_001314057.2 100% 100%
2 human 4154 MBNL1 muscleblind like splicing r... XM_017006435.1 99.6% 99.4% 1_4delATGCinsA
3 human 4154 MBNL1 muscleblind like splicing r... XM_005247474.3 89.7% 89.7% 0_1ins105
4 human 4154 MBNL1 muscleblind like splicing r... XM_005247475.3 89.7% 89.7% 0_1ins105
5 human 4154 MBNL1 muscleblind like splicing r... XM_005247477.5 89.7% 89.7% 0_1ins105
6 human 4154 MBNL1 muscleblind like splicing r... XM_011512848.2 89.7% 89.7% 0_1ins105
7 human 4154 MBNL1 muscleblind like splicing r... XM_005247464.5 85.5% 85.5% 1_171del;173_174delAAinsTG
8 human 4154 MBNL1 muscleblind like splicing r... XM_017006423.2 85.5% 85.5% 1_171del;173_174delAAinsTG
9 human 4154 MBNL1 muscleblind like splicing r... XM_017006424.2 85.5% 85.5% 1_171del;173_174delAAinsTG
10 human 4154 MBNL1 muscleblind like splicing r... XM_005247476.5 84.5% 84.5% 0_1ins105;529_530ins54
11 human 4154 MBNL1 muscleblind like splicing r... XM_017006440.2 84.5% 84.5% 0_1ins105;529_530ins54
12 human 4154 MBNL1 muscleblind like splicing r... NM_207293.1 82.5% 82.5% 1_171del;173_174delAAinsTG;1014_1015ins36
13 human 4154 MBNL1 muscleblind like splicing r... XM_005247466.5 82.5% 82.5% 1_171del;173_174delAAinsTG;1014_1015ins36
14 human 4154 MBNL1 muscleblind like splicing r... XM_017006425.2 82.5% 82.5% 1_171del;173_174delAAinsTG;1014_1015ins36
15 human 4154 MBNL1 muscleblind like splicing r... XM_017006426.1 82.5% 82.5% 1_171del;173_174delAAinsTG;1014_1015ins36
16 human 4154 MBNL1 muscleblind like splicing r... NM_021038.5 81% 81% 1_171del;173_174delAAinsTG;805_806ins54
17 human 4154 MBNL1 muscleblind like splicing r... XM_017006427.2 81% 81% 1_171del;173_174delAAinsTG;805_806ins54
18 human 4154 MBNL1 muscleblind like splicing r... XM_017006428.1 81% 81% 1_171del;173_174delAAinsTG;805_806ins54
19 human 4154 MBNL1 muscleblind like splicing r... XM_017006429.1 81% 81% 1_171del;173_174delAAinsTG;805_806ins54
20 human 4154 MBNL1 muscleblind like splicing r... XM_017006441.1 81% 81% 0_1ins105;529_530ins54;684_685ins36
21 human 4154 MBNL1 muscleblind like splicing r... XM_017006442.1 81% 81% 0_1ins105;529_530ins54;684_685ins36
22 human 4154 MBNL1 muscleblind like splicing r... XM_005247457.5 79.4% 79.3% 1_265del;267_268insG
23 human 4154 MBNL1 muscleblind like splicing r... XM_005247458.5 79.4% 79.3% 1_265del;267_268insG
24 human 4154 MBNL1 muscleblind like splicing r... XM_005247460.4 79.4% 79.3% 1_265del;267_268insG
25 human 4154 MBNL1 muscleblind like splicing r... XM_006713639.1 79.4% 79.3% 1_265del;267_268insG
26 human 4154 MBNL1 muscleblind like splicing r... NM_207292.3 78% 78% (many diffs)
27 human 4154 MBNL1 muscleblind like splicing r... XM_017006430.2 78% 78% (many diffs)
28 human 4154 MBNL1 muscleblind like splicing r... XM_017006431.2 78% 78% (many diffs)
29 human 4154 MBNL1 muscleblind like splicing r... XM_005247461.5 76.6% 76.5% 1_265del;267_268insG;1107_1108ins36
30 human 4154 MBNL1 muscleblind like splicing r... XM_017006432.2 75.5% 73.7% (many diffs)
31 human 4154 MBNL1 muscleblind like splicing r... XM_005247462.5 75.2% 75.1% 1_265del;267_268insG;898_899ins54
32 human 4154 MBNL1 muscleblind like splicing r... NM_001363870.1 72.5% 70.3% (many diffs)
33 human 4154 MBNL1 muscleblind like splicing r... XM_017006433.2 72.5% 70.3% (many diffs)
34 human 4154 MBNL1 muscleblind like splicing r... XM_005247463.5 72.4% 72.3% (many diffs)
35 human 4154 MBNL1 muscleblind like splicing r... NM_207297.1 71% 69.2% (many diffs)
36 human 4154 MBNL1 muscleblind like splicing r... XM_005247471.5 71% 69.2% (many diffs)
37 human 4154 MBNL1 muscleblind like splicing r... XM_017006436.2 71% 69.2% (many diffs)
38 human 4154 MBNL1 muscleblind like splicing r... XM_005247465.5 70.1% 68.4% (many diffs)
39 human 4154 MBNL1 muscleblind like splicing r... XM_017006443.1 69.3% 66.8% (many diffs)
40 human 4154 MBNL1 muscleblind like splicing r... XM_017006437.2 68% 65.8% (many diffs)
41 human 4154 MBNL1 muscleblind like splicing r... XM_005247467.5 67.3% 65.2% (many diffs)
42 human 4154 MBNL1 muscleblind like splicing r... XM_017006438.2 65.5% 65.5% (many diffs)
43 human 4154 MBNL1 muscleblind like splicing r... NM_207295.1 64% 64% (many diffs)
44 human 4154 MBNL1 muscleblind like splicing r... XM_011512847.3 64% 64% (many diffs)
45 human 4154 MBNL1 muscleblind like splicing r... XM_011512843.3 63.6% 63.5% 1_265del;267_268insG;437_438ins204
46 human 4154 MBNL1 muscleblind like splicing r... XM_005247468.5 63.2% 61.1% (many diffs)
47 human 4154 MBNL1 muscleblind like splicing r... NM_207296.1 61.9% 62% (many diffs)
48 human 4154 MBNL1 muscleblind like splicing r... NM_207294.1 61% 61% (many diffs)
49 human 4154 MBNL1 muscleblind like splicing r... XM_011512849.3 61% 61% (many diffs)
50 human 4154 MBNL1 muscleblind like splicing r... XM_011512845.3 59.5% 59.3% (many diffs)
51 human 4154 MBNL1 muscleblind like splicing r... XM_011512846.3 56.7% 56.6% (many diffs)
52 human 4154 MBNL1 muscleblind like splicing r... XR_001740148.2 38.6% (many diffs)
53 human 4154 MBNL1 muscleblind like splicing r... XR_001740147.2 20.2% (many diffs)
54 human 4154 MBNL1 muscleblind like splicing r... XR_001740145.2 17.9% (many diffs)
55 human 4154 MBNL1 muscleblind like splicing r... XR_001740146.2 16.3% (many diffs)
56 human 4154 MBNL1 muscleblind like splicing r... XR_002959535.1 11% (many diffs)
57 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501797.3 85.1% 89.2% (many diffs)
58 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501798.3 85.1% 89.2% (many diffs)
59 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501799.3 85.1% 89.2% (many diffs)
60 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319665.1 85.1% 89.2% (many diffs)
61 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501777.1 81% 85% (many diffs)
62 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253713.2 79.8% 83.9% (many diffs)
63 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319667.1 79.8% 83.9% (many diffs)
64 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501780.1 78% 82% (many diffs)
65 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501786.3 76.8% 80.5% (many diffs)
66 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501787.3 76.8% 80.5% (many diffs)
67 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501788.3 76.8% 80.5% (many diffs)
68 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501789.3 76.8% 80.5% (many diffs)
69 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501790.3 76.8% 80.5% (many diffs)
70 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501791.3 76.8% 80.5% (many diffs)
71 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501793.3 76.8% 80.5% (many diffs)
72 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501794.3 76.8% 80.5% (many diffs)
73 mouse 56758 Mbnl1 muscleblind like splicing f... NM_020007.4 76.5% 80.5% (many diffs)
74 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319668.1 76.3% 80.4% (many diffs)
75 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501772.1 74.1% 77.8% (many diffs)
76 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501784.2 73.5% 77.5% (many diffs)
77 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501773.1 71.3% 75% (many diffs)
78 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501774.2 70% 73.6% (many diffs)
79 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253711.2 69.9% 71.5% (many diffs)
80 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253709.2 69.7% 73.7% (many diffs)
81 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253710.2 68.2% 70.2% (many diffs)
82 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001310514.1 68% 69.8% (many diffs)
83 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319663.1 67.8% 71.7% (many diffs)
84 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501775.1 67.2% 70.9% (many diffs)
85 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253708.2 66.5% 68.7% (many diffs)
86 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501778.1 64.9% 67% (many diffs)
87 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319664.1 64.8% 68.7% (many diffs)
88 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501796.2 63.5% 65.3% (many diffs)
89 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501781.1 62.2% 63.9% (many diffs)
90 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501782.1 62% 65.6% (many diffs)
91 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319661.1 60.8% 62.9% (many diffs)
92 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501783.1 59.3% 62.9% (many diffs)
93 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319662.1 58.1% 59.8% (many diffs)
94 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319666.1 54.8% 56.6% (many diffs)
95 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501795.1 50.1% 51.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1095
ORF length:
1029
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggg ccgttgctcc agggagaact gcaaatatct tcatccaccc ccacatttaa
121aaacgcagtt ggagataaat ggacgcaata acttgattca gcagaagaac atggccatgt
181tggcccagca aatgcaacta gccaatgcca tgatgcctgg tgccccatta caacccgtgc
241caatgttttc agttgcacca agcttagcca ccaatgcatc agcagccgcc tttaatccct
301atctgggacc tgtttctcca agcctggtcc cggcagagat cttgccgact gcaccaatgt
361tggttacagg gaatccgggt gtccctgtac ctgcagctgc tgcagctgct gcacagaaat
421taatgcgaac agacagactt gaggtatgtc gagagtacca acgtggcaat tgcaaccgag
481gagaaaatga ttgtcggttt gctcatcctg ctgacagcac aatgattgac accaatgaca
541acacagtcac tgtgtgtatg gattacatca aagggagatg ctctcgggaa aagtgcaaat
601actttcatcc ccctgcacat ttgcaagcca agatcaaggc tgcccaatac caggtcaacc
661aggctgcagc tgcacaggct gcagccaccg cagctgccat gactcagtcg gctgtcaaat
721cactgaagcg acccctcgag gcaacctttg acctgggaat tcctcaagct gtacttcccc
781cattaccaaa gaggcctgct cttgaaaaaa ccaacggtgc caccgcagtc tttaacactg
841gtattttcca ataccaacag gctctagcca acatgcagtt acaacagcat acagcatttc
901tcccaccagg ctcaatattg tgcatgacac ccgctacaag tgttgttccc atggtgcacg
961gtgctacgcc agccactgtg tccgcagcaa caacatctgc cacaagtgtt cccttcgctg
1021caacagccac agccaaccag atacccataa tatctgccga acatctgact agccacaagt
1081atgttaccca gatgtnncca actttcttgt acaaagttgg cattataaga aagcattgct
1141tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)