Construct: ORF TRCN0000467871

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003839.1_s317c1
Derived from:
ccsbBroadEn_00980
DNA Barcode:
TGTGAAGGTACTCGGCAACAGACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MBNL1 (4154)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467871
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4154 MBNL1 muscleblind like splicing r... NM_021038.5 100% 100%
2 human 4154 MBNL1 muscleblind like splicing r... XM_017006427.2 100% 100%
3 human 4154 MBNL1 muscleblind like splicing r... XM_017006428.1 100% 100%
4 human 4154 MBNL1 muscleblind like splicing r... XM_017006429.1 100% 100%
5 human 4154 MBNL1 muscleblind like splicing r... NM_207292.3 96.8% 96.8% 960_961ins36
6 human 4154 MBNL1 muscleblind like splicing r... XM_017006430.2 96.8% 96.8% 960_961ins36
7 human 4154 MBNL1 muscleblind like splicing r... XM_017006431.2 96.8% 96.8% 960_961ins36
8 human 4154 MBNL1 muscleblind like splicing r... XM_005247464.5 95.5% 95.5% 806_859del
9 human 4154 MBNL1 muscleblind like splicing r... XM_017006423.2 95.5% 95.5% 806_859del
10 human 4154 MBNL1 muscleblind like splicing r... XM_017006424.2 95.5% 95.5% 806_859del
11 human 4154 MBNL1 muscleblind like splicing r... NM_207293.1 92.5% 92.5% 806_859del;1014_1015ins36
12 human 4154 MBNL1 muscleblind like splicing r... XM_005247466.5 92.5% 92.5% 806_859del;1014_1015ins36
13 human 4154 MBNL1 muscleblind like splicing r... XM_017006425.2 92.5% 92.5% 806_859del;1014_1015ins36
14 human 4154 MBNL1 muscleblind like splicing r... XM_017006426.1 92.5% 92.5% 806_859del;1014_1015ins36
15 human 4154 MBNL1 muscleblind like splicing r... XM_005247462.5 92.4% 92.4% 175_267del
16 human 4154 MBNL1 muscleblind like splicing r... XM_005247463.5 89.5% 89.5% 175_267del;1053_1054ins36
17 human 4154 MBNL1 muscleblind like splicing r... NM_207297.1 89.5% 87.6% 996_997ins95;1026_1027ins25
18 human 4154 MBNL1 muscleblind like splicing r... XM_005247471.5 89.5% 87.6% 996_997ins95;1026_1027ins25
19 human 4154 MBNL1 muscleblind like splicing r... XM_017006436.2 89.5% 87.6% 996_997ins95;1026_1027ins25
20 human 4154 MBNL1 muscleblind like splicing r... XM_005247457.5 88.6% 88.6% 175_267del;899_952del
21 human 4154 MBNL1 muscleblind like splicing r... XM_005247458.5 88.6% 88.6% 175_267del;899_952del
22 human 4154 MBNL1 muscleblind like splicing r... XM_005247460.4 88.6% 88.6% 175_267del;899_952del
23 human 4154 MBNL1 muscleblind like splicing r... XM_006713639.1 88.6% 88.6% 175_267del;899_952del
24 human 4154 MBNL1 muscleblind like splicing r... XM_017006437.2 86.3% 84% 956_957ins131;990_991ins25
25 human 4154 MBNL1 muscleblind like splicing r... XM_005247461.5 85.8% 85.8% 175_267del;899_952del;1107_1108ins36
26 human 4154 MBNL1 muscleblind like splicing r... XM_017006432.2 85.5% 83.7% 806_859del;1050_1051ins95;1080_1081ins25
27 human 4154 MBNL1 muscleblind like splicing r... NM_001363870.1 82.5% 80.2% 806_859del;1010_1011ins131;1044_1045ins25
28 human 4154 MBNL1 muscleblind like splicing r... XM_017006433.2 82.5% 80.2% 806_859del;1010_1011ins131;1044_1045ins25
29 human 4154 MBNL1 muscleblind like splicing r... NM_207295.1 82.1% 82.1% 344_345ins204
30 human 4154 MBNL1 muscleblind like splicing r... XM_011512847.3 82.1% 82.1% 344_345ins204
31 human 4154 MBNL1 muscleblind like splicing r... XM_017006435.1 81.4% 81% (many diffs)
32 human 4154 MBNL1 muscleblind like splicing r... NM_001314057.2 81% 81% 0_1ins171;2_3delTGinsAA;635_688del
33 human 4154 MBNL1 muscleblind like splicing r... XM_005247468.5 79.9% 77.7% 175_267del;1049_1050ins131;1083_1084ins25
34 human 4154 MBNL1 muscleblind like splicing r... XM_005247465.5 79.3% 77.7% (many diffs)
35 human 4154 MBNL1 muscleblind like splicing r... NM_207294.1 79% 79% 344_345ins204;756_757ins36
36 human 4154 MBNL1 muscleblind like splicing r... XM_011512849.3 79% 79% 344_345ins204;756_757ins36
37 human 4154 MBNL1 muscleblind like splicing r... NM_207296.1 79% 79.4% 805_806ins190;902_965del
38 human 4154 MBNL1 muscleblind like splicing r... XM_005247467.5 76.5% 74.5% (many diffs)
39 human 4154 MBNL1 muscleblind like splicing r... XM_011512845.3 76% 76% 175_267del;437_438ins204
40 human 4154 MBNL1 muscleblind like splicing r... XM_005247476.5 75.9% 75.9% 0_1ins276
41 human 4154 MBNL1 muscleblind like splicing r... XM_017006440.2 75.9% 75.9% 0_1ins276
42 human 4154 MBNL1 muscleblind like splicing r... XM_017006438.2 75.5% 75.5% 344_345ins204;602_655del;810_811ins36
43 human 4154 MBNL1 muscleblind like splicing r... XM_011512846.3 73.1% 73.1% 175_267del;437_438ins204;849_850ins36
44 human 4154 MBNL1 muscleblind like splicing r... XM_011512843.3 72.8% 72.8% 175_267del;437_438ins204;695_748del
45 human 4154 MBNL1 muscleblind like splicing r... XM_017006441.1 72.7% 72.7% 0_1ins276;684_685ins36
46 human 4154 MBNL1 muscleblind like splicing r... XM_017006442.1 72.7% 72.7% 0_1ins276;684_685ins36
47 human 4154 MBNL1 muscleblind like splicing r... XM_005247474.3 72.5% 72.5% 0_1ins276;530_583del
48 human 4154 MBNL1 muscleblind like splicing r... XM_005247475.3 72.5% 72.5% 0_1ins276;530_583del
49 human 4154 MBNL1 muscleblind like splicing r... XM_005247477.5 72.5% 72.5% 0_1ins276;530_583del
50 human 4154 MBNL1 muscleblind like splicing r... XM_011512848.2 72.5% 72.5% 0_1ins276;530_583del
51 human 4154 MBNL1 muscleblind like splicing r... XM_017006443.1 62.3% 60% 0_1ins276;680_681ins131;714_715ins25
52 human 4154 MBNL1 muscleblind like splicing r... XR_001740148.2 43.2% (many diffs)
53 human 4154 MBNL1 muscleblind like splicing r... XR_001740147.2 22.6% (many diffs)
54 human 4154 MBNL1 muscleblind like splicing r... XR_001740145.2 20% (many diffs)
55 human 4154 MBNL1 muscleblind like splicing r... XR_001740146.2 19.5% (many diffs)
56 human 4154 MBNL1 muscleblind like splicing r... XR_002959535.1 13.6% (many diffs)
57 mouse 56758 Mbnl1 muscleblind like splicing f... NM_020007.4 94.8% 99.4% (many diffs)
58 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501784.2 91.7% 96.3% (many diffs)
59 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501777.1 90.5% 95% (many diffs)
60 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501780.1 87.5% 92% (many diffs)
61 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501774.2 86.4% 90.6% (many diffs)
62 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319663.1 85.7% 90.3% (many diffs)
63 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253708.2 84.3% 87.1% (many diffs)
64 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501775.1 83.6% 87.8% (many diffs)
65 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501772.1 82.9% 86.9% (many diffs)
66 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319664.1 82.6% 87.1% (many diffs)
67 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501796.2 81.2% 83.5% (many diffs)
68 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501773.1 80.1% 84.2% (many diffs)
69 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501782.1 78.2% 82.3% (many diffs)
70 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001310514.1 77.5% 79.8% (many diffs)
71 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319661.1 76.9% 79.4% (many diffs)
72 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501783.1 75.3% 79.4% (many diffs)
73 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319662.1 74% 76.1% (many diffs)
74 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501778.1 73.7% 76.2% (many diffs)
75 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319666.1 72.1% 74.4% (many diffs)
76 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253713.2 71.7% 75.3% (many diffs)
77 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319667.1 71.7% 75.3% (many diffs)
78 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501781.1 71% 73% (many diffs)
79 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319668.1 68.5% 72.2% (many diffs)
80 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501797.3 68.5% 72% (many diffs)
81 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501798.3 68.5% 72% (many diffs)
82 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501799.3 68.5% 72% (many diffs)
83 mouse 56758 Mbnl1 muscleblind like splicing f... XM_017319665.1 68.5% 72% (many diffs)
84 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501795.1 65.7% 67.8% (many diffs)
85 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501786.3 62.7% 65.9% (many diffs)
86 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501787.3 62.7% 65.9% (many diffs)
87 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501788.3 62.7% 65.9% (many diffs)
88 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501789.3 62.7% 65.9% (many diffs)
89 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501790.3 62.7% 65.9% (many diffs)
90 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501791.3 62.7% 65.9% (many diffs)
91 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501793.3 62.7% 65.9% (many diffs)
92 mouse 56758 Mbnl1 muscleblind like splicing f... XM_006501794.3 62.7% 65.9% (many diffs)
93 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253709.2 62.6% 66.2% (many diffs)
94 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253710.2 61.2% 63% (many diffs)
95 mouse 56758 Mbnl1 muscleblind like splicing f... NM_001253711.2 55.5% 56.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgttagtgtc acaccaattc gggacacaaa atggctaaca ctggaagtat
121gtagagagtt ccagaggggg acttgctcac ggccagacac ggaatgtaaa tttgcacatc
181cttcgaaaag ctgccaagtt gaaaatggac gagtaatcgc ctgctttgat tcattgaaag
241gccgttgctc cagggagaac tgcaaatatc ttcatccacc cccacattta aaaacgcagt
301tggagataaa tggacgcaat aacttgattc agcagaagaa catggccatg ttggcccagc
361aaatgcaact agccaatgcc atgatgcctg gtgccccatt acaacccgtg ccaatgtttt
421cagttgcacc aagcttagcc accaatgcat cagcagccgc ctttaatccc tatctgggac
481ctgtttctcc aagcctggtc ccggcagaga tcttgccgac tgcaccaatg ttggttacag
541ggaatccggg tgtccctgta cctgcagctg ctgcagctgc tgcacagaaa ttaatgcgaa
601cagacagact tgaggtatgt cgagagtacc aacgtggcaa ttgcaaccga ggagaaaatg
661attgtcggtt tgctcatcct gctgacagca caatgattga caccaatgac aacacagtca
721ctgtgtgtat ggattacatc aaagggagat gctctcggga aaagtgcaaa tactttcatc
781cccctgcaca tttgcaagcc aagatcaagg ctgcccaata ccaggtcaac caggctgcag
841ctgcacaggc tgcagccacc gcagcTGCCA TGGGAATTCC TCAAGCTGTA CTTCCCCCAT
901TACCAAAGAG GCCTGCTCTT GAAAAAACCA ACGGTGCCAC CGCAGTCTTT AACACTGGTA
961TTTTCCAATA CCAACAGGCT CTAGCCAACA TGCAGTTACA ACAGCATACA GCATTTCTCC
1021CACCAGGCTC AATATTGTGC ATGACACCCG CTACAAGTGT TGTTCCCATG GTGCACGGTG
1081CTACGCCAGC CACTGTGTCC GCAGCAACAA CATCTGCCAC AAGTGTTCCC TTCGCTGCAA
1141CAGCCACAGC CAACCAGATA CCCATAATAT CTGCCGAACA TCTGACTAGC CACAAGTATG
1201TTACCCAGAT GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1261ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1321CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATGT GAAGGTACTC GGCAACAGAC
1381CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)