Transcript: Human XM_005251794.4

PREDICTED: Homo sapiens ankyrin repeat and sterile alpha motif domain containing 6 (ANKS6), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ANKS6 (203286)
Length:
3191
CDS:
39..2696

Additional Resources:

NCBI RefSeq record:
XM_005251794.4
NBCI Gene record:
ANKS6 (203286)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005251794.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000122619 GCGATTTCTGAACTGAACGCA pLKO.1 2523 CDS 100% 0.750 1.050 N ANKS6 n/a
2 TRCN0000143107 GATGAACTGACTGGAATCCTT pLKO.1 2364 CDS 100% 3.000 2.400 N ANKS6 n/a
3 TRCN0000121799 CTGACTGGAATCCTTAAGAAA pLKO.1 2370 CDS 100% 5.625 3.938 N ANKS6 n/a
4 TRCN0000144123 CCATTCACAACTTTCACTCTT pLKO.1 2578 CDS 100% 4.950 3.465 N ANKS6 n/a
5 TRCN0000143590 GAGCTGGGAATTAAGACAGAT pLKO.1 2478 CDS 100% 4.950 3.465 N ANKS6 n/a
6 TRCN0000139747 CTCCATCTGGAACTTCCACTA pLKO.1 2203 CDS 100% 4.050 2.835 N ANKS6 n/a
7 TRCN0000139349 GAACAAGAGGTGGACATGGAA pLKO.1 2424 CDS 100% 3.000 2.100 N ANKS6 n/a
8 TRCN0000144021 CAATTCTGGAAACTTCAACCA pLKO.1 1916 CDS 100% 2.640 1.848 N ANKS6 n/a
9 TRCN0000139664 CACTCTTCCTTTGAGAGCAGT pLKO.1 2592 CDS 100% 2.640 1.848 N ANKS6 n/a
10 TRCN0000140121 GAGTGACAAGCTGAAAGCAGT pLKO.1 1688 CDS 100% 2.640 1.848 N ANKS6 n/a
11 TRCN0000143484 GAATTAAGACAGATGGGTCCA pLKO.1 2485 CDS 100% 2.160 1.512 N ANKS6 n/a
12 TRCN0000143664 CACTTGAGAAATATCAGCCCA pLKO.1 2395 CDS 100% 0.660 0.462 N ANKS6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005251794.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13400 pDONR223 100% 53.1% 52.8% None (many diffs) n/a
2 ccsbBroadEn_14432 pDONR223 100% 25% 19.5% None (many diffs) n/a
3 ccsbBroad304_14432 pLX_304 0% 25% 19.5% V5 (not translated due to frame shift) (many diffs) n/a
4 TRCN0000467484 AAAATAGTGATAACCTATCGGGCG pLX_317 48.5% 25% 19.5% V5 (not translated due to frame shift) (many diffs) n/a
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