Transcript: Human XM_005268157.3

PREDICTED: Homo sapiens cyclin dependent kinase like 1 (CDKL1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDKL1 (8814)
Length:
2374
CDS:
641..1717

Additional Resources:

NCBI RefSeq record:
XM_005268157.3
NBCI Gene record:
CDKL1 (8814)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005268157.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000356033 GCTGTTGCATCACCCATATTT pLKO_005 1483 CDS 100% 15.000 21.000 N CDKL1 n/a
2 TRCN0000006071 CACGAAACATTCCGTGATTAA pLKO.1 1045 CDS 100% 13.200 18.480 N CDKL1 n/a
3 TRCN0000356032 CTGGACCGAGTGACTACTATA pLKO_005 1098 CDS 100% 13.200 18.480 N CDKL1 n/a
4 TRCN0000006073 CCGGTGGATGTTTGGGCAATT pLKO.1 1187 CDS 100% 10.800 15.120 N CDKL1 n/a
5 TRCN0000356034 CCACATGGACCCTACTCAAAG pLKO_005 1447 CDS 100% 10.800 8.640 N CDKL1 n/a
6 TRCN0000355982 ACGAAACATTCCGTGATTAAG pLKO_005 1046 CDS 100% 13.200 9.240 N CDKL1 n/a
7 TRCN0000195639 CCTACTCAAAGGCTGACATGT pLKO.1 1457 CDS 100% 4.950 3.465 N CDKL1 n/a
8 TRCN0000197130 GTCAGATTGTGGCCATCAAGA pLKO.1 723 CDS 100% 4.950 3.465 N CDKL1 n/a
9 TRCN0000195401 CATCCAAGTTGCAGTACCTAC pLKO.1 1602 CDS 100% 4.050 2.835 N CDKL1 n/a
10 TRCN0000006072 CCTGAAGATATGGAACCACTT pLKO.1 1370 CDS 100% 4.050 2.835 N CDKL1 n/a
11 TRCN0000006069 CCAGCAAGTGTTTAGCACGAA pLKO.1 1318 CDS 100% 2.640 1.848 N CDKL1 n/a
12 TRCN0000006070 GCCATCAAGAAGTTTCTGGAA pLKO.1 734 CDS 100% 2.640 1.848 N CDKL1 n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 567 5UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 567 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005268157.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02021 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02021 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000477527 TGTAACCGATCCCACACATCGTGG pLX_317 34.4% 100% 100% V5 n/a
4 TRCN0000470267 AATACCCGCGTAGATCGAACGGGA pLX_317 41.7% 83.4% 82.4% V5 (many diffs) n/a
5 ccsbBroadEn_14914 pDONR223 100% 83.3% 82.1% None (many diffs) n/a
6 ccsbBroad304_14914 pLX_304 0% 83.3% 82.1% V5 (many diffs) n/a
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