Transcript: Mouse XM_006506193.2

PREDICTED: Mus musculus contactin 4 (Cntn4), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Cntn4 (269784)
Length:
6134
CDS:
534..3614

Additional Resources:

NCBI RefSeq record:
XM_006506193.2
NBCI Gene record:
Cntn4 (269784)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006506193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000434975 AGCGCTGAGCTTAGTGTAATA pLKO_005 1719 CDS 100% 13.200 18.480 N Cntn4 n/a
2 TRCN0000113632 GCAGTCAATACAGTTCCAGAT pLKO.1 2463 CDS 100% 4.050 5.670 N Cntn4 n/a
3 TRCN0000419582 AGCAAGGAACACTCAACATAA pLKO_005 1627 CDS 100% 13.200 9.240 N Cntn4 n/a
4 TRCN0000415017 GAAATCCAGTCCCAACTATTC pLKO_005 1285 CDS 100% 10.800 7.560 N Cntn4 n/a
5 TRCN0000415822 TGTTGACATTGGTATGGATTT pLKO_005 737 CDS 100% 10.800 7.560 N Cntn4 n/a
6 TRCN0000113633 CCACACCACTAATCCTGAGAA pLKO.1 1159 CDS 100% 4.950 3.465 N Cntn4 n/a
7 TRCN0000113631 GCCAGAAGACATAAGTCGAAT pLKO.1 1344 CDS 100% 4.950 3.465 N Cntn4 n/a
8 TRCN0000113634 GCTGGAAGTTACACCTGCATA pLKO.1 1947 CDS 100% 4.950 3.465 N Cntn4 n/a
9 TRCN0000222607 GCAGCAAATCTGAACTGGTTA pLKO.1 2665 CDS 100% 4.950 2.970 N CNTN4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006506193.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13287 pDONR223 100% 61.3% 64.9% None (many diffs) n/a
2 ccsbBroad304_13287 pLX_304 0% 61.3% 64.9% V5 (many diffs) n/a
3 TRCN0000471672 GCGGTGACCCCTCTGTTTCCTGGG pLX_317 19.2% 61.3% 64.9% V5 (many diffs) n/a
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