Construct: ORF TRCN0000471672
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006851.1_s317c1
- Derived from:
- ccsbBroadEn_13287
- DNA Barcode:
- GCGGTGACCCCTCTGTTTCCTGGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNTN4 (152330)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471672
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152330 | CNTN4 | contactin 4 | NM_001206956.1 | 99.8% | 100% | (many diffs) |
| 2 | human | 152330 | CNTN4 | contactin 4 | NM_175613.2 | 99.6% | 99.8% | (many diffs) |
| 3 | human | 152330 | CNTN4 | contactin 4 | XM_011533431.2 | 68.5% | 68.6% | (many diffs) |
| 4 | human | 152330 | CNTN4 | contactin 4 | XM_006713004.4 | 67.8% | 68% | (many diffs) |
| 5 | human | 152330 | CNTN4 | contactin 4 | XM_017005785.1 | 67.8% | 68% | (many diffs) |
| 6 | human | 152330 | CNTN4 | contactin 4 | XM_017005786.1 | 67.8% | 68% | (many diffs) |
| 7 | human | 152330 | CNTN4 | contactin 4 | XM_017005787.1 | 67.8% | 68% | (many diffs) |
| 8 | human | 152330 | CNTN4 | contactin 4 | NM_001206955.1 | 67.8% | 67.9% | (many diffs) |
| 9 | human | 152330 | CNTN4 | contactin 4 | NM_001350095.1 | 67.8% | 67.9% | (many diffs) |
| 10 | human | 152330 | CNTN4 | contactin 4 | NM_175607.3 | 67.8% | 67.9% | (many diffs) |
| 11 | human | 152330 | CNTN4 | contactin 4 | XM_011533425.3 | 67.8% | 67.9% | (many diffs) |
| 12 | human | 152330 | CNTN4 | contactin 4 | XM_011533427.2 | 67.8% | 67.9% | (many diffs) |
| 13 | human | 152330 | CNTN4 | contactin 4 | XM_011533428.2 | 67.8% | 67.9% | (many diffs) |
| 14 | human | 152330 | CNTN4 | contactin 4 | XM_011533429.2 | 67.8% | 67.9% | (many diffs) |
| 15 | human | 152330 | CNTN4 | contactin 4 | XM_011533430.2 | 67.8% | 67.9% | (many diffs) |
| 16 | human | 152330 | CNTN4 | contactin 4 | XM_017005782.1 | 67.8% | 67.9% | (many diffs) |
| 17 | human | 152330 | CNTN4 | contactin 4 | XM_017005783.1 | 67.8% | 67.9% | (many diffs) |
| 18 | human | 152330 | CNTN4 | contactin 4 | XM_017005784.2 | 67.8% | 67.9% | (many diffs) |
| 19 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506196.2 | 61.3% | 64.9% | (many diffs) |
| 20 | mouse | 269784 | Cntn4 | contactin 4 | XM_017321598.1 | 61.3% | 64.9% | (many diffs) |
| 21 | mouse | 269784 | Cntn4 | contactin 4 | NM_001109749.1 | 61.3% | 64.9% | (many diffs) |
| 22 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506193.2 | 61.3% | 64.9% | (many diffs) |
| 23 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506194.3 | 61.3% | 64.9% | (many diffs) |
| 24 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506195.3 | 61.3% | 64.9% | (many diffs) |
| 25 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506198.2 | 52.3% | 55.2% | (many diffs) |
| 26 | mouse | 269784 | Cntn4 | contactin 4 | XM_006506197.2 | 52.3% | 55.1% | (many diffs) |
| 27 | mouse | 269784 | Cntn4 | contactin 4 | NM_001109751.1 | 32.7% | 34% | (many diffs) |
| 28 | mouse | 269784 | Cntn4 | contactin 4 | NM_173004.3 | 32.7% | 34% | (many diffs) |
| 29 | mouse | 269784 | Cntn4 | contactin 4 | XM_017321599.1 | 32.7% | 34% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2157
- ORF length:
- 2091
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaaaatgtc ttttgggaat gtaaagcaaa tggaaggcct aagcctacat 121 acaagtggct aaaaaatggc gaacctctgc taactcggga tagaattcaa attgagcaag 181 gaacactcaa cataacaata gtgaacctct cagatgctgg catgtatcag tgtttggcag 241 agaataaaca tggagttatc ttttccaacg cagagcttag tgttatagct gtaggtccag 301 atttttcaag aacactcttg aaaagagtaa ctcttgtcaa agtgggaggt gaagttgtca 361 ttgagtgtaa gccaaaagcg tctccaaaac ctgtttacac ctggaagaaa ggaagggata 421 tattaaaaga aaatgaaaga attaccattt ctgaagatgg aaacctcaga atcatcaacg 481 ttactaaatc agacgctggg agttatacct gtatagccac taaccatttt ggaactgcta 541 gcagtactgg aaacttggta gtgaaagatc caacaagggt aatggtaccc ccttccagta 601 tggatgtcac tgttggagag agtattgttt taccgtgcca ggtaacgcat gatcactcgc 661 tagacatcgt gtttacttgg tcatttaatg gacacctgat agactttgac agagatgggg 721 accactttga aagagttgga ggggattcag ctggtgattt gatgatccga aacatccaac 781 tgaagcatgc tgggaaatat gtctgcatgg tccaaacaag tgtggacagg ctatctgctg 841 ctgcagacct gattgtaaga ggtcctccag gtcccccaga ggctgtgaca atagacgaaa 901 tcacagatac cactgctcag ctctcctgga gacccgggcc tgacaatcac agccccatca 961 ccatgtatgt cattcaagcc aggactccgt tctccgtggg ctggcaagca gtcagtacag 1021 tcccagaact cattgatggg aagacattca cagcgaccgt ggtgggtttg aacccttggg 1081 ttgaatatga attccgcaca gttgcagcca acgtgattgg gattggggag cccagccgcc 1141 cctcagagaa acggagaaca gaagaagctc tccccgaagt cacaccagcg aatgtcagtg 1201 gtggcggagg cagcaaatct gaactggtta taacctggga gacggtccct gaggaattac 1261 agaatggtcg tggctttggt tatgtggtgg ccttccggcc ctacggtaaa atgatctgga 1321 tgctgacagt gctggcctca gctgacgcct ctagatacgt gttcaggaat gagagcgtgc 1381 accccttctc tccctttgag gttaaagtag gtgtcttcaa caacaaagga gaaggccctt 1441 tcagtcccac cacggtggtg tattctgcag aagaagaacc caccaaacca ccagccagta 1501 tctttgccag aagtctttct gccacagata ttgaagtttt ctgggcctcc ccactggaga 1561 agaatagagg acgaatacaa ggttatgagg ttaaatattg gagacatgaa gacaaagaag 1621 aaaatgctag aaaaatacga acagttggaa atcagacatc aacaaaaatc acgaacttaa 1681 aaggcagtgt gctgtatcac ttagctgtca aggcatataa ttctgctggg acaggcccct 1741 ctagtgcaac agtcaatgtg aCAACCCGAA AGCCACCACC AAGTCAACCC CCCGGAAACA 1801 TCATATGGAA TTCATCAGAC TCCAAAATTA TCCTGAATTG GGATCAAGTG AAGGCCCTGG 1861 ATAATGAGTC GGAAGTAAAA GGATACAAAG TCTTGTACAG ATGGAACAGA CAAAGCAGCA 1921 CATCTGTCAT TGAAACAAAT AAAACATCGG TGGAGCTTTC TTTGCCTTTC GATGAAGATT 1981 ATATAATAGA AATTAAGCCA TTCAGCGACG GAGGAGATGG CAGCAGCAGT GAACAAATTC 2041 GAATTCCAAA GATATCAAAT GCCTACGCGA GAGGATCTGG GGCTTCCACT TCGAATGCAT 2101 GTACGCTGTC AGCCATCAGT ACAATAATGA TTTCCCTCAC AGCTAGGTCC AGTTTATGCC 2161 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2221 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2281 ATATATCTTG TGGAAAGGAC GAGCGGTGAC CCCTCTGTTT CCTGGGACGC GTTAAGTCga 2341 caatcaacct ctggattaca aaatttgtga aagatt