Transcript: Mouse XM_006507443.2

PREDICTED: Mus musculus PTPRF interacting protein, binding protein 2 (liprin beta 2) (Ppfibp2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ppfibp2 (19024)
Length:
3514
CDS:
240..2996

Additional Resources:

NCBI RefSeq record:
XM_006507443.2
NBCI Gene record:
Ppfibp2 (19024)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006507443.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000328494 AGTGTCAGTGCTCCCGTATTA pLKO_005 1734 CDS 100% 13.200 18.480 N Ppfibp2 n/a
2 TRCN0000029864 GCAGACATTAGTAGATGTTTA pLKO.1 3226 3UTR 100% 13.200 18.480 N Ppfibp2 n/a
3 TRCN0000328555 GCAGACATTAGTAGATGTTTA pLKO_005 3226 3UTR 100% 13.200 18.480 N Ppfibp2 n/a
4 TRCN0000345411 GTGTCAGTGCTCCCGTATTAG pLKO_005 1735 CDS 100% 13.200 18.480 N Ppfibp2 n/a
5 TRCN0000029867 CTCAGCATCAAATGTGCTATT pLKO.1 2406 CDS 100% 10.800 8.640 N Ppfibp2 n/a
6 TRCN0000353485 CTCAGCATCAAATGTGCTATT pLKO_005 2406 CDS 100% 10.800 8.640 N Ppfibp2 n/a
7 TRCN0000029866 CCCAACAGCTACCTACATAAA pLKO.1 584 CDS 100% 13.200 9.240 N Ppfibp2 n/a
8 TRCN0000328554 CCCAACAGCTACCTACATAAA pLKO_005 584 CDS 100% 13.200 9.240 N Ppfibp2 n/a
9 TRCN0000029868 GCACGGCTAGAAGGAGATAAA pLKO.1 681 CDS 100% 13.200 9.240 N Ppfibp2 n/a
10 TRCN0000328495 ACCACGGTGCTGCTAGTAATG pLKO_005 640 CDS 100% 10.800 7.560 N Ppfibp2 n/a
11 TRCN0000029865 GCTGGGAAGATATAGTCTCAT pLKO.1 1771 CDS 100% 4.950 3.465 N Ppfibp2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006507443.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07245 pDONR223 100% 82.4% 84.7% None (many diffs) n/a
2 ccsbBroad304_07245 pLX_304 0% 82.4% 84.7% V5 (many diffs) n/a
3 TRCN0000491463 ACCCCGGAACCGCCACCCGCAATG pLX_317 10.6% 82.4% 84.7% V5 (many diffs) n/a
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