Transcript: Mouse XM_006518366.1

PREDICTED: Mus musculus guanosine monophosphate reductase 2 (Gmpr2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Gmpr2 (105446)
Length:
1500
CDS:
123..1169

Additional Resources:

NCBI RefSeq record:
XM_006518366.1
NBCI Gene record:
Gmpr2 (105446)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006518366.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000042169 CCTGACTGTCTTGAGTGTCTA pLKO.1 399 CDS 100% 4.950 3.960 N Gmpr2 n/a
2 TRCN0000334736 CCTGACTGTCTTGAGTGTCTA pLKO_005 399 CDS 100% 4.950 3.960 N Gmpr2 n/a
3 TRCN0000042170 CTACGGAATGAGTTCTGAAAT pLKO.1 920 CDS 100% 13.200 9.240 N Gmpr2 n/a
4 TRCN0000334690 CTACGGAATGAGTTCTGAAAT pLKO_005 920 CDS 100% 13.200 9.240 N Gmpr2 n/a
5 TRCN0000042171 CCAGCAAGTGAATCCAATCTT pLKO.1 1133 CDS 100% 5.625 3.938 N Gmpr2 n/a
6 TRCN0000323412 CCAGCAAGTGAATCCAATCTT pLKO_005 1133 CDS 100% 5.625 3.938 N Gmpr2 n/a
7 TRCN0000042172 GCTAACGGCTACTCTGAACAT pLKO.1 513 CDS 100% 4.950 3.465 N Gmpr2 n/a
8 TRCN0000351447 GCTAACGGCTACTCTGAACAT pLKO_005 513 CDS 100% 4.950 3.465 N Gmpr2 n/a
9 TRCN0000042168 GCTGCTAAACTGAAGGAACTA pLKO.1 1083 CDS 100% 4.950 3.465 N Gmpr2 n/a
10 TRCN0000351415 GCTGCTAAACTGAAGGAACTA pLKO_005 1083 CDS 100% 4.950 3.465 N Gmpr2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006518366.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03268 pDONR223 100% 89% 95.1% None (many diffs) n/a
2 ccsbBroad304_03268 pLX_304 0% 89% 95.1% V5 (many diffs) n/a
3 TRCN0000478673 CTCGAATAAAATTCAGAGCCAGTG pLX_317 33.8% 89% 95.1% V5 (many diffs) n/a
4 ccsbBroadEn_11971 pDONR223 100% 75.8% 80.9% None (many diffs) n/a
5 ccsbBroad304_11971 pLX_304 0% 75.8% 80.9% V5 (many diffs) n/a
6 TRCN0000471192 GGACTCGGTATCTAAATAAGCCCC pLX_317 33.5% 75.8% 80.9% V5 (many diffs) n/a
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