Transcript: Mouse XM_006525149.2

PREDICTED: Mus musculus CAP-GLY domain containing linker protein family, member 4 (Clip4), transcript variant X7, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Clip4 (78785)
Length:
4030
CDS:
255..2228

Additional Resources:

NCBI RefSeq record:
XM_006525149.2
NBCI Gene record:
Clip4 (78785)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006525149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000180352 GCTCTCCGAGATATGGAATAT pLKO.1 1723 CDS 100% 13.200 18.480 N Clip4 n/a
2 TRCN0000340365 GGCTCATGGCATCGGTGATAT pLKO_005 608 CDS 100% 13.200 18.480 N Clip4 n/a
3 TRCN0000340439 TTTAGTTGCTCTCCGAGATAT pLKO_005 1716 CDS 100% 13.200 18.480 N Clip4 n/a
4 TRCN0000180885 GCTCACAAGCTCTAACGAAAT pLKO.1 2000 CDS 100% 10.800 15.120 N Clip4 n/a
5 TRCN0000340438 GGTTTACTTTACTTAGCTATT pLKO_005 2308 3UTR 100% 10.800 8.640 N Clip4 n/a
6 TRCN0000340441 ATGCCACTTGCAGCGACTTTA pLKO_005 790 CDS 100% 13.200 9.240 N Clip4 n/a
7 TRCN0000183903 CCTGCACATTGCAGCACATAA pLKO.1 824 CDS 100% 13.200 9.240 N Clip4 n/a
8 TRCN0000340363 TGTGGGTGACAAGCGCTATTT pLKO_005 2114 CDS 100% 13.200 9.240 N Clip4 n/a
9 TRCN0000180826 GCAGCAAATAACAGTCACCAT pLKO.1 1533 CDS 100% 2.640 1.848 N Clip4 n/a
10 TRCN0000135332 CTATTTCACCTGTAAGCCGAA pLKO.1 2129 CDS 100% 2.160 1.512 N CLIP4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006525149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08954 pDONR223 100% 77.7% 82.6% None (many diffs) n/a
2 ccsbBroad304_08954 pLX_304 0% 77.7% 82.6% V5 (many diffs) n/a
3 TRCN0000467345 CTTTCTCAACCTAGGCCGGTAGAG pLX_317 14.7% 77.7% 82.6% V5 (many diffs) n/a
4 ccsbBroadEn_15989 pDONR223 0% 52% 55.1% None (many diffs) n/a
5 ccsbBroad304_15989 pLX_304 0% 52% 55.1% V5 (many diffs) n/a
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