Transcript: Mouse XM_006530721.2

PREDICTED: Mus musculus kinesin family member C3 (Kifc3), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kifc3 (16582)
Length:
3561
CDS:
146..2962

Additional Resources:

NCBI RefSeq record:
XM_006530721.2
NBCI Gene record:
Kifc3 (16582)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006530721.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000305965 TCAGAGGTCTGGGCTATATTT pLKO_005 3373 3UTR 100% 15.000 12.000 N Kifc3 n/a
2 TRCN0000090890 GCTGCGCAAGAAATGCCATAA pLKO.1 1750 CDS 100% 10.800 8.640 N Kifc3 n/a
3 TRCN0000325220 GCTGCGCAAGAAATGCCATAA pLKO_005 1750 CDS 100% 10.800 8.640 N Kifc3 n/a
4 TRCN0000090889 CCCACCTGTTAAGTATGTCAT pLKO.1 1213 CDS 100% 4.950 3.465 N Kifc3 n/a
5 TRCN0000090892 GACTCTGTACTCCCTCAGATT pLKO.1 2722 CDS 100% 4.950 3.465 N Kifc3 n/a
6 TRCN0000325221 GACTCTGTACTCCCTCAGATT pLKO_005 2722 CDS 100% 4.950 3.465 N Kifc3 n/a
7 TRCN0000090891 GATGACATCAACAAGGTGTTT pLKO.1 2318 CDS 100% 4.950 3.465 N Kifc3 n/a
8 TRCN0000325222 GATGACATCAACAAGGTGTTT pLKO_005 2318 CDS 100% 4.950 3.465 N Kifc3 n/a
9 TRCN0000116466 CAACGACTACAATGGGCTCAA pLKO.1 1597 CDS 100% 4.050 2.835 N KIFC3 n/a
10 TRCN0000305902 AGGCCATCGAGGAGGTTAATA pLKO_005 1686 CDS 100% 15.000 9.000 N Kifc3 n/a
11 TRCN0000090888 CCTACCATGAATGCAGGAGAT pLKO.1 3015 3UTR 100% 4.050 2.430 N Kifc3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006530721.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488029 AACGCATGTCCGTGTACATCGGTC pLX_317 14.8% 64.9% 69.7% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_14686 pDONR223 97% 64.8% 69.6% None (many diffs) n/a
3 ccsbBroad304_14686 pLX_304 0% 64.8% 69.6% V5 (many diffs) n/a
4 TRCN0000481469 CGTTTGCCGTTTGTCCTGATCTTA pLX_317 25.8% 62.6% 67.2% V5 (many diffs) n/a
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