Construct: ORF TRCN0000488029
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021749.1_s317c1
- DNA Barcode:
- AACGCATGTCCGTGTACATCGGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- KIFC3 (3801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488029
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001130099.1 | 99.9% | 99.8% | 755G>T;1530G>A |
| 2 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318714.1 | 99.9% | 99.8% | 755G>T;1530G>A |
| 3 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318715.2 | 99.9% | 99.8% | 755G>T;1530G>A |
| 4 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450269.1 | 99.9% | 99.8% | 755G>T;1530G>A |
| 5 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318713.1 | 99.4% | 99.4% | 755G>T;1095_1096insGTGTTCCAG;1521G>A |
| 6 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523078.1 | 98.8% | 98.7% | (many diffs) |
| 7 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523079.1 | 98.8% | 98.7% | (many diffs) |
| 8 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450265.1 | 98.8% | 98.7% | (many diffs) |
| 9 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450266.1 | 98.8% | 98.7% | (many diffs) |
| 10 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450267.1 | 98.8% | 98.7% | (many diffs) |
| 11 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450268.1 | 98.8% | 98.7% | (many diffs) |
| 12 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318712.2 | 94.7% | 94.7% | 1_111del;866G>T;1641G>A |
| 13 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318711.2 | 89.3% | 89.3% | 1_243del;998G>T;1773G>A |
| 14 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023227.1 | 89.3% | 89.3% | 1_243del;998G>T;1773G>A |
| 15 | human | 3801 | KIFC3 | kinesin family member C3 | XM_005255944.1 | 88.5% | 88.3% | (many diffs) |
| 16 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023226.1 | 88.5% | 88.3% | (many diffs) |
| 17 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023225.1 | 88.4% | 88.2% | (many diffs) |
| 18 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001130100.2 | 83% | 83% | 1_417del;1172G>T;1947G>A |
| 19 | human | 3801 | KIFC3 | kinesin family member C3 | NM_005550.4 | 82.3% | 82.2% | (many diffs) |
| 20 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318710.2 | 80.9% | 80.8% | 1_483del;1238G>T;2013G>A |
| 21 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023223.1 | 80.2% | 80.1% | (many diffs) |
| 22 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523077.1 | 79.7% | 79.5% | (many diffs) |
| 23 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523076.1 | 78.4% | 78.4% | 1_564del;1319G>T;2094G>A |
| 24 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523075.1 | 77.8% | 77.6% | (many diffs) |
| 25 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023222.1 | 75.2% | 75.1% | (many diffs) |
| 26 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023224.1 | 75.2% | 75.1% | (many diffs) |
| 27 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023221.1 | 69.3% | 69.3% | 1_906del;1661G>T;2436G>A |
| 28 | human | 3801 | KIFC3 | kinesin family member C3 | XM_005255937.1 | 68.9% | 68.7% | (many diffs) |
| 29 | human | 3801 | KIFC3 | kinesin family member C3 | XM_006721188.1 | 67.1% | 67% | (many diffs) |
| 30 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023220.1 | 64.9% | 64.8% | (many diffs) |
| 31 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023219.1 | 63.6% | 63.4% | (many diffs) |
| 32 | human | 3801 | KIFC3 | kinesin family member C3 | NR_134678.1 | 54.6% | (many diffs) | |
| 33 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_001145832.1 | 88.5% | 95.3% | (many diffs) |
| 34 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_017312586.1 | 80.7% | 86.9% | (many diffs) |
| 35 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530726.3 | 80% | 86% | (many diffs) |
| 36 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530724.3 | 76.5% | 82.2% | (many diffs) |
| 37 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_001145831.1 | 76.1% | 81.9% | (many diffs) |
| 38 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_010631.2 | 73.8% | 79.4% | (many diffs) |
| 39 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_017312585.1 | 73.2% | 78.7% | (many diffs) |
| 40 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530723.3 | 69.1% | 74.3% | (many diffs) |
| 41 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530721.2 | 64.9% | 69.7% | (many diffs) |
| 42 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530722.2 | 64.9% | 69.7% | (many diffs) |
| 43 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530720.3 | 62% | 66.8% | (many diffs) |
| 44 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530718.3 | 61.7% | 66.3% | (many diffs) |
| 45 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530717.3 | 61.6% | 66.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2130
- ORF length:
- 2061
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtggagaat gagcgactga ggcaggagat gcggcgctgt gaggccgagc 121 tgcaagagct gcgcacaaag ccagcaggtc cctgcccagg ttgtgagcac agccaggaga 181 gcgcccagct ccgtgacaag ctgtcccagc tgcagctgga gatggcggaa agcaaaggca 241 tgctgtcaga gctgaaccta gaggtgcagc agaagaccga ccggctggct gaggtggagc 301 tgcgactcaa ggactgcctg gctgagaagg cacaggagga ggagcggctt agtcggcgcc 361 tgcgtgacag ccacgagacc attgccagcc tgcgggccca gtccccacct gtcaagtatg 421 tcatcaagac agtggaggtg gagtcgtcca agaccaagca ggccctcagc gagtcccagg 481 cccggaacca gcacctgcag gagcaggtgg ctatgcagag gcaggtgctg aaggagatgg 541 aacagcagct gcagagctca caccagctga ccgcgcggct ccgggcgcag attgccatgt 601 acgagtcaga gctggagcgg gcccatgggc agatgctgga ggagatgcag tccctggaag 661 aggacaagaa ccgggccatt gaggaggcct ttgccagagc ccaggtggag atgaaggctg 721 tgcacgagaa tctagcaggc gtccggacca acttgctgac cttgcagccg gcactgcgga 781 ccctcaccaa cgactacaat gggctcaagc ggcaggtgcg cgtcttccca ctgctgctgc 841 aggaggccct caggagtgtc aaggccgaga taggccaggc catcgaggag gtcaacagca 901 acaaccagga gctgctgcgc aagtaccgcc gcgagctgca gctgcgtaag aagtgccaca 961 atgagctcgt gcggctgaaa gggaacatcc gagtgattgc tcgtgtccgg ccagtcacca 1021 aagaggatgg ggaaggacct gaggccacca atgctgtgac tttcgatgcc gacgacgact 1081 ccatcatcca cctgctgcac aagggcaagc ctgtgtcctt cgagctggac aaggtcttct 1141 ccccacaggc ctcgcagcag gacgtgttcc aggaggtgca ggccctggtc acctcttgca 1201 ttgatggctt caatgtctgc atctttgcgt acggccagac gggcgccggc aagacgtaca 1261 cgatggaggg gaccgctgag aacccaggta tcaaccagcg ggccctgcag ctgctcttct 1321 ccgaggtgca ggagaaggcg tctgactggg agtacaccat caccgtcagc gctgcggaga 1381 tctacaatga ggtcctcagg gacctgctag ggaaagagcc tcaggaaaaa ctggagatcc 1441 ggctgtgccc agacggcagt gggcagctgt atgtaccagg gctgactgag ttccaagtgc 1501 agagcgtgga cgacatcaac aaggtgtttg agtttggcca cactaatcgc acgaccgagt 1561 tcaccaacct gaacgagcac agctcccgct cgcacgcact gctcatcgtg acggtgcgag 1621 gcgtggactg cagcacaggc ctccgcacca cggggaagct gaacctggtg gacttggctg 1681 gctcggagcg cgtgggcaag tcgggggccg agggcagccg cctgcgggag gcgcagcaca 1741 tcaacaagtc gctgtcggct ctgggggacg tcattgctgc cctgcgctcc cgccagggcc 1801 acgtgccctt ccgcaactcc aagctcacct accTGCTGCA GGATTCGCTT AGTGGTGACA 1861 GCAAGACCCT CATGGTGGTA CAGGTGTCCC CCGTGGAGAA GAACACTAGC GAGACGCTCT 1921 ATTCCCTCAA GTTTGCTGAG AGGGTGCGCT CTGTGGAGCT GGGGCCTGGG CTACGCAGGG 1981 CAGAGCTTGG GTCCTGGTCA AGCCAGGAGC ATCTAGAGTG GGAGCCGGCT TGTCAGACGC 2041 CACAGCCCTC GGCACGGGCC CACTCAGCCC CCAGCTCTGG GACCAGTAGC CGCCCTGGAT 2101 CCATCCGGAG GAAGCTGCAG CCCTCGGCCT AGAACCCAGC TTTCTTGTAC AAAGTGGTTG 2161 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2221 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2281 CGCATGTCCG TGTACATCGG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2341 ttgtgaaaga tt