Construct: ORF TRCN0000481469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008185.1_s317c1
- Derived from:
- ccsbBroadEn_14686
- DNA Barcode:
- CGTTTGCCGTTTGTCCTGATCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIFC3 (3801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001130099.1 | 96.2% | 96.2% | 755G>T;1530G>A;1986_2061delinsC |
| 2 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318714.1 | 96.2% | 96.2% | 755G>T;1530G>A;1986_2061delinsC |
| 3 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318715.2 | 96.2% | 96.2% | 755G>T;1530G>A;1986_2061delinsC |
| 4 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450269.1 | 96.2% | 96.2% | 755G>T;1530G>A;1986_2061delinsC |
| 5 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318713.1 | 95.7% | 95.7% | (many diffs) |
| 6 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523078.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 7 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523079.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 8 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450265.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 9 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450266.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 10 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450267.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 11 | human | 3801 | KIFC3 | kinesin family member C3 | XM_024450268.1 | 95.2% | 95.2% | 755G>T;1530G>A;1986_2082delinsC |
| 12 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318712.2 | 91.2% | 91.2% | (many diffs) |
| 13 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318711.2 | 86% | 86% | (many diffs) |
| 14 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023227.1 | 86% | 86% | (many diffs) |
| 15 | human | 3801 | KIFC3 | kinesin family member C3 | XM_005255944.1 | 85.2% | 85.2% | (many diffs) |
| 16 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023226.1 | 85.2% | 85.2% | (many diffs) |
| 17 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023225.1 | 85.1% | 85.1% | (many diffs) |
| 18 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001130100.2 | 80% | 80% | (many diffs) |
| 19 | human | 3801 | KIFC3 | kinesin family member C3 | NM_005550.4 | 79.3% | 79.3% | (many diffs) |
| 20 | human | 3801 | KIFC3 | kinesin family member C3 | NM_001318710.2 | 77.9% | 77.9% | (many diffs) |
| 21 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023223.1 | 77.3% | 77.3% | (many diffs) |
| 22 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523077.1 | 76.7% | 76.7% | (many diffs) |
| 23 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523076.1 | 75.5% | 75.5% | (many diffs) |
| 24 | human | 3801 | KIFC3 | kinesin family member C3 | XM_011523075.1 | 74.9% | 74.9% | (many diffs) |
| 25 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023222.1 | 72.4% | 72.4% | (many diffs) |
| 26 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023224.1 | 72.4% | 72.4% | (many diffs) |
| 27 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023221.1 | 66.8% | 66.8% | (many diffs) |
| 28 | human | 3801 | KIFC3 | kinesin family member C3 | XM_005255937.1 | 66.3% | 66.3% | (many diffs) |
| 29 | human | 3801 | KIFC3 | kinesin family member C3 | XM_006721188.1 | 64.6% | 64.6% | (many diffs) |
| 30 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023220.1 | 62.5% | 62.5% | (many diffs) |
| 31 | human | 3801 | KIFC3 | kinesin family member C3 | XM_017023219.1 | 61.2% | 61.2% | (many diffs) |
| 32 | human | 3801 | KIFC3 | kinesin family member C3 | NR_134678.1 | 52.6% | (many diffs) | |
| 33 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_001145832.1 | 85.4% | 91.8% | (many diffs) |
| 34 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_017312586.1 | 77.9% | 83.7% | (many diffs) |
| 35 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530726.3 | 77.2% | 83% | (many diffs) |
| 36 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530724.3 | 73.8% | 79.3% | (many diffs) |
| 37 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_001145831.1 | 73.4% | 78.9% | (many diffs) |
| 38 | mouse | 16582 | Kifc3 | kinesin family member C3 | NM_010631.2 | 71.2% | 76.5% | (many diffs) |
| 39 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_017312585.1 | 70.6% | 75.9% | (many diffs) |
| 40 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530723.3 | 66.7% | 71.7% | (many diffs) |
| 41 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530721.2 | 62.6% | 67.2% | (many diffs) |
| 42 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530722.2 | 62.6% | 67.2% | (many diffs) |
| 43 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530720.3 | 59.9% | 64.3% | (many diffs) |
| 44 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530718.3 | 59.5% | 63.9% | (many diffs) |
| 45 | mouse | 16582 | Kifc3 | kinesin family member C3 | XM_006530717.3 | 59.5% | 63.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2052
- ORF length:
- 1986
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggagaatgag cgactgaggc aggagatgcg gcgctgtgag gccgagctgc 121 aagagctgcg cacaaagcca gcaggtccct gcccaggttg tgagcacagc caggagagcg 181 cccagctccg tgacaagctg tcccagctgc agctggagat ggcggaaagc aaaggcatgc 241 tgtcagagct gaacctagag gtgcagcaga agaccgaccg gctggctgag gtggagctgc 301 gactcaagga ctgcctggct gagaaggcac aggaggagga gcggcttagt cggcgcctgc 361 gtgacagcca cgagaccatt gccagcctgc gggcccagtc cccacctgtc aagtatgtca 421 tcaagacagt ggaggtggag tcgtccaaga ccaagcaggc cctcagcgag tcccaggccc 481 ggaaccagca cctgcaggag caggtggcta tgcagaggca ggtgctgaag gagatggaac 541 agcagctgca gagctcacac cagctgaccg cgcggctccg ggcgcagatt gccatgtacg 601 agtcagagct ggagcgggcc catgggcaga tgctggagga gatgcagtcc ctggaagagg 661 acaagaaccg ggccattgag gaggcctttg ccagagccca ggtggagatg aaggctgtgc 721 acgagaatct agcaggcgtc cggaccaact tgctgacctt gcagccggca ctgcggaccc 781 tcaccaacga ctacaatggg ctcaagcggc aggtgcgcgt cttcccactg ctgctgcagg 841 aggccctcag gagtgtcaag gccgagatag gccaggccat cgaggaggtc aacagcaaca 901 accaggagct gctgcgcaag taccgccgcg agctgcagct gcgtaagaag tgccacaatg 961 agctcgtgcg gctgaaaggg aacatccgag tgattgctcg tgtccggcca gtcaccaaag 1021 aggatgggga aggacctgag gccaccaatg ctgtgacttt cgatgccgac gacgactcca 1081 tcatccacct gctgcacaag ggcaagcctg tgtccttcga gctggacaag gtcttctccc 1141 cacaggcctc gcagcaggac gtgttccagg aggtgcaggc cctggtcacc tcttgcattg 1201 atggcttcaa tgtctgcatc tttgcgtacg gccagacggg cgccggcaag acgtacacga 1261 tggaggggac cgctgagaac ccaggtatca accagcgggc cctgcagctg ctcttctccg 1321 aggtgcagga gaaggcgtct gactgggagt acaccatcac cgtcagcgct gcggagatct 1381 acaatgaggt cctcagggac ctgctaggga aagagcctca ggaaaaactg gagatccggc 1441 tgtgcccaga cggcagtggg cagctgtatg taccagggct gactgagttc caagtgcaga 1501 gcgtggacga catcaacaag gtgtttgagt ttggccacac taaTCGCACG ACCGAGTTCA 1561 CCAACCTGAA CGAGCACAGC TCCCGCTCGC ACGCACTGCT CATCGTGACG GTGCGAGGCG 1621 TGGACTGCAG CACAGGCCTC CGCACCACGG GGAAGCTGAA CCTGGTGGAC TTGGCTGGCT 1681 CGGAGCGCGT GGGCAAGTCG GGGGCCGAGG GCAGCCGCCT GCGGGAGGCG CAGCACATCA 1741 ACAAGTCGCT GTCGGCTCTG GGGGACGTCA TTGCTGCCCT GCGCTCCCGC CAGGGCCACG 1801 TGCCCTTCCG CAACTCCAAG CTCACCTACC TGCTGCAGGA TTCGCTTAGT GGTGACAGCA 1861 AGACCCTCAT GGTGGTACAG GTGTCCCCCG TGGAGAAGAA CACTAGCGAG ACGCTCTATT 1921 CCCTCAAGTT TGCTGAGAGG GTGCGCTCTG TGGAGCTGGG GCCTGGGCTA CGCAGGGCAG 1981 AGCTTGGGTC CTGGTCAAGC CAGGAGCATC TAGAGTGGGA GCCGGCTTGT CAGACGCCAC 2041 AGCCCTCGGC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2101 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2161 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT TTGCCGTTTG TCCTGATCTT 2221 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t