Transcript: Mouse XM_006538545.3

PREDICTED: Mus musculus FGR proto-oncogene, Src family tyrosine kinase (Fgr), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fgr (14191)
Length:
2960
CDS:
494..2047

Additional Resources:

NCBI RefSeq record:
XM_006538545.3
NBCI Gene record:
Fgr (14191)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006538545.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000023470 GCGATCACATAAAGCATTATA pLKO.1 1032 CDS 100% 15.000 21.000 N Fgr n/a
2 TRCN0000378655 ACGGCTGAAGAACGCTATTTC pLKO_005 572 CDS 100% 13.200 18.480 N Fgr n/a
3 TRCN0000378537 ATGGAACGCATGAACTATATC pLKO_005 1574 CDS 100% 13.200 18.480 N Fgr n/a
4 TRCN0000023469 CGGCACTACATGGAAGTGAAT pLKO.1 1127 CDS 100% 4.950 3.960 N Fgr n/a
5 TRCN0000363395 TCATCCTTTGTCTAGTTATTT pLKO_005 2431 3UTR 100% 15.000 10.500 N Fgr n/a
6 TRCN0000023473 CTATGGTAGCTTGCTGGATTT pLKO.1 1471 CDS 100% 10.800 7.560 N Fgr n/a
7 TRCN0000023471 CTTCGGAAAGATCAGTAGAAA pLKO.1 892 CDS 100% 5.625 3.938 N Fgr n/a
8 TRCN0000023472 CAGTCTTTCCTGGAAGACTAT pLKO.1 1982 CDS 100% 0.495 0.347 N Fgr n/a
9 TRCN0000199653 GCCTTTCTCATTCGGGAAAGC pLKO.1 956 CDS 100% 0.405 0.284 N FGR n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006538545.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00564 pDONR223 100% 82.8% 84.1% None (many diffs) n/a
2 ccsbBroad304_00564 pLX_304 0% 82.8% 84.1% V5 (many diffs) n/a
3 TRCN0000478102 CGGACTTCGCGTGCGTAGGTCTTC pLX_317 21.4% 82.8% 84.1% V5 (many diffs) n/a
4 ccsbBroadEn_14641 pDONR223 0% 82.8% 84.1% None (many diffs) n/a
5 ccsbBroad304_14641 pLX_304 0% 82.8% 84.1% V5 (many diffs) n/a
6 TRCN0000472499 CTCTCTAATATTTACACGCAAGGT pLX_317 26.9% 82.8% 84.1% V5 (many diffs) n/a
7 TRCN0000492193 ACATCTCGAATCGGTTGGCTTTCC pLX_317 11% 82.8% 84.1% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV