Transcript: Human XM_006711253.2

PREDICTED: Homo sapiens G protein-coupled receptor 161 (GPR161), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GPR161 (23432)
Length:
8020
CDS:
202..2031

Additional Resources:

NCBI RefSeq record:
XM_006711253.2
NBCI Gene record:
GPR161 (23432)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006711253.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255425 ACTCGATTCTTCATGTGAAAG pLKO_005 1778 CDS 100% 10.800 15.120 N GPR161 n/a
2 TRCN0000358079 CGGTATTATCGGGAACCATTT pLKO_005 1468 CDS 100% 10.800 15.120 N GPR161 n/a
3 TRCN0000255423 GTTGGTCATCCGTGGAGTTTG pLKO_005 935 CDS 100% 10.800 15.120 N GPR161 n/a
4 TRCN0000014228 CCGGTATTATCGGGAACCATT pLKO.1 1467 CDS 100% 4.950 6.930 N GPR161 n/a
5 TRCN0000014230 CGAGTTCAAATGGATGTGTGT pLKO.1 957 CDS 100% 2.640 3.696 N GPR161 n/a
6 TRCN0000014231 CCTGATCTATGGACTCTGGAA pLKO.1 1404 CDS 100% 2.640 2.112 N GPR161 n/a
7 TRCN0000255424 AGAAGGAGCTCGGTGACATTT pLKO_005 1720 CDS 100% 13.200 9.240 N GPR161 n/a
8 TRCN0000255427 ATGGGACCAGAAGCATCTAAA pLKO_005 2262 3UTR 100% 13.200 9.240 N GPR161 n/a
9 TRCN0000358086 AGTCCACAGCTTCAGCATTTC pLKO_005 2205 3UTR 100% 10.800 7.560 N GPR161 n/a
10 TRCN0000357997 CCCAGTTCATCGCCATCATTG pLKO_005 527 CDS 100% 10.800 7.560 N GPR161 n/a
11 TRCN0000255426 TCCGCAGGGAATGGATCTTTG pLKO_005 707 CDS 100% 10.800 7.560 N GPR161 n/a
12 TRCN0000014232 GCCATCATTGTCATCACCATT pLKO.1 538 CDS 100% 4.950 3.465 N GPR161 n/a
13 TRCN0000014229 CCTCAGCAACAAGTTCGTCTT pLKO.1 621 CDS 100% 4.050 2.835 N GPR161 n/a
14 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6418 3UTR 100% 4.950 2.475 Y ERAP2 n/a
15 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6419 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006711253.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02766 pDONR223 100% 86.8% 86.8% None 1_240del n/a
2 ccsbBroad304_02766 pLX_304 0% 86.8% 86.8% V5 1_240del n/a
3 TRCN0000471229 GAAGTCACCCTACTTATAAGCTCG pLX_317 26.5% 86.8% 86.8% V5 1_240del n/a
4 TRCN0000488861 CGGATCCTCTTTTATTGTACGGTT pLX_317 21.7% 86.8% 86.8% V5 (not translated due to prior stop codon) 1_240del n/a
5 TRCN0000487991 GGCATAGCGAACAGCCTCTTCGTC pLX_317 20% 86.8% 86.7% V5 1_240del;1827_1828insG n/a
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