Construct: ORF TRCN0000488688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019917.1_s317c1
- DNA Barcode:
- CACCGAGCGGTTAATCGTTAAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOAT1 (6646)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_003101.6 | 99.9% | 99.8% | 1650_1651insG |
| 2 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | XM_011509911.1 | 99.9% | 99.8% | 1650_1651insG |
| 3 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_001252511.1 | 89.2% | 89.1% | 0_1ins175;2delT;1476_1477insG |
| 4 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NM_001252512.1 | 88.1% | 88% | 0_1ins195;1455_1456insG |
| 5 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | XM_011509912.1 | 60% | 60% | 990_990delGinsCTTTGGT;993_994ins652 |
| 6 | human | 6646 | SOAT1 | sterol O-acyltransferase 1 | NR_045530.2 | 22.6% | (many diffs) | |
| 7 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | NM_009230.3 | 82.2% | 85.2% | (many diffs) |
| 8 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | XM_006496719.3 | 77.6% | 81.3% | (many diffs) |
| 9 | mouse | 20652 | Soat1 | sterol O-acyltransferase 1 | XM_011238778.2 | 77.6% | 81.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1722
- ORF length:
- 1653
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggtgggtgaa gagaagatgt ctctaagaaa ccggctgtca aagtccaggg 121 aaaatcctga ggaagatgaa gaccagagaa accctgcaaa ggagtcccta gagacaccta 181 gtaatggtcg aattgacata aaacagttga tagcaaagaa gataaagttg acagcagagg 241 cagaggaatt gaagccattt tttatgaagg aagttggcag tcactttgat gattttgtga 301 ccaatctcat tgaaaagtca gcatcattag ataatggtgg gtgcgctctc acaacctttt 361 ctgttcttga aggagagaaa aacaaccata gagcgaagga tttgagagca cctccagaac 421 aaggaaagat ttttattgca aggcgctctc tcttagatga actgcttgaa gtggaccaca 481 tcagaacaat atatcacatg tttattgccc tcctcattct ctttatcctc agcacacttg 541 tagtagatta cattgatgaa ggaaggctgg tgcttgagtt cagcctcctg tcttatgctt 601 ttggcaaatt tcctaccgtt gtttggacct ggtggatcat gttcctgtct acattttcag 661 ttccctattt tctgtttcaa cattgggcca ctggctatag caagagttct catccgctga 721 tccgttctct cttccatggc tttcttttca tgatcttcca gattggagtt ctaggttttg 781 gaccaacata tgttgtgtta gcatatacac tgccaccagc ttcccggttc atcattatat 841 tcgagcagat tcgttttgta atgaaggccc actcatttgt cagagagaac gtgcctcggg 901 tactaaattc agctaaggag aaatcaagca ctgttccaat acctacagtc aaccagtatt 961 tgtacttctt atttgctcct acccttatct accgtgacag ctatcccagg aatcccactg 1021 taagatgggg ttatgtcgct atgaagtttg cacaggtctt tggttgcttt ttctatgtgt 1081 actacatctt tgaaaggctt tgtgccccct tgtttcggaa tatcaaacag gagcccttca 1141 gcgctcgtgt tctggtccta tgtgtattta actccatctt gccaggtgtg ctgattctct 1201 tccttacttt ttttgccttt ttgcactgct ggctcaatgc ctttgctgag atgttacgct 1261 ttggtgacag gatgttctat aaggattggt ggaactccac gtcatactcc aactattata 1321 gaacctggaa tgtggtggtc catgactGGC TATATTACTA TGCTTACAAG GACTTTCTCT 1381 GGTTTTTCTC CAAGAGATTC AAATCTGCTG CCATGTTAGC TGTCTTTGCT GTATCTGCTG 1441 TAGTACACGA ATATGCCTTG GCTGTTTGCT TGAGCTTTTT CTATCCCGTG CTCTTCGTGC 1501 TCTTCATGTT CTTTGGAATG GCTTTCAACT TCATTGTCAA TGATAGTCGG AAAAAGCCGA 1561 TTTGGAATGT TCTGATGTGG ACTTCTCTTT TCTTGGGCAA TGGAGTCTTA CTCTGCTTTT 1621 ATTCTCAAGA ATGGTATGCA CGTCAGCACT GTCCTCTGAA AAATCCCACA TTTTTGGATT 1681 ATGTCCGGCC ACGTTCCTGG ACTTGTCGTT ACGTGTTTGA CCCAGCTTTC TTGTACAAAG 1741 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1801 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1861 ACGACACCGA GCGGTTAATC GTTAAAGTAC GCGTTAAGTC gacaatcaac ctctggatta 1921 caaaatttgt gaaagatt