Construct: ORF ccsbBroad304_14755

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018821.1_s304c1, BRDN0000459305
Derived from:
ccsbBroadEn_14755
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PHKG1 (5260)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14755
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5260 PHKG1 phosphorylase kinase cataly... NM_006213.4 99.6% 34.8% (many diffs)
2 human 5260 PHKG1 phosphorylase kinase cataly... NM_001258460.1 97.3% 32.5% (many diffs)
3 human 5260 PHKG1 phosphorylase kinase cataly... NM_001258459.1 92% 32.2% (many diffs)
4 human 5260 PHKG1 phosphorylase kinase cataly... XM_017012324.2 89.2% 31.2% (many diffs)
5 human 5260 PHKG1 phosphorylase kinase cataly... XM_005271772.5 79.5% 14.7% (many diffs)
6 human 5260 PHKG1 phosphorylase kinase cataly... XM_017012325.2 76.8% 9% (many diffs)
7 human 5260 PHKG1 phosphorylase kinase cataly... XM_017012326.2 71.2% 13.1% (many diffs)
8 human 5260 PHKG1 phosphorylase kinase cataly... XM_017012327.2 64.7% 6.7% (many diffs)
9 human 5260 PHKG1 phosphorylase kinase cataly... NR_047689.1 51.7% (many diffs)
10 mouse 18682 Phkg1 phosphorylase kinase gamma 1 NM_011079.3 86.9% 32.4% (many diffs)
11 mouse 18682 Phkg1 phosphorylase kinase gamma 1 XM_011240871.2 63.1% 7.2% (many diffs)
12 mouse 18682 Phkg1 phosphorylase kinase gamma 1 NR_145486.1 34.4% (many diffs)
13 mouse 18682 Phkg1 phosphorylase kinase gamma 1 NR_145487.1 34.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
546
ORF length:
477
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gacccgggac gaggcactgc cggactctca ttctgcacag gacttctatg
121agaattatga gcccaaagag atcctgggca ggggcgttag cagtgtggtc aggcgatgca
181tccacaagcc cacgagccag gagtacgccg tgaaggtcat cgacgtcacc ggtggaggca
241gcttcagccc ggaggaggtg cgggagctgc gagaagccac gctgaaggag gtggacatcc
301tgcgcaaggt ctcagggcac cccaacatca tacagctgaa ggacacttat gagaccaaca
361ctttcttctt cttggtgttt gacctgatga agagagggga gctctttgac tacctcactg
421agaaggtcac cttgagtgag aaggaaacca gaaaanatca tgcgagctct gctggaggtg
481atctgcacct tgcacaaact caacatcgtg caccgggacc tgaagcccga gaacattctc
541ttggatgaca acatgaacat caagctcaca gactttggct tttcctgcca gctggagccg
601ggagagaggc tgcgagaggt ctgtgggacc cccagttacc tggcccctga gattatcgag
661tgctccatga atgaggacca cccgggctac gggaaagagg tggacatgtg gagcactggc
721gtcatcatgt acacgctgct ggccggctcc ccgcccttct ggcaccggaa gcagatgctg
781atgctgagga tgatcatgag cggcaactac cagtttggct cgcccgagtg ggatgattac
841tcggacaccg tgaaggacct ggttctcccg attcctggtg gtgcaacccc agaaccgcta
901cacagcggaa gaggccttgg cacacccctt cttccagcag tacttggtgg aggaagtgcg
961gcacttcagc ccccggggga agttcaaggt gatcgctctg accgtgctgg cttcagtgcg
1021gatctactac cagtaccgcc gggtgaagcc tgtgacccgg gagatcgtca tccgagaccc
1081ctatgccctc cggcctctgc gccggctcat cgacgcctac gctttccgaa tctatggcca
1141ctgggtgaag aaggggcagc agcagaaccg ggcagccctt ttcgagaaca cacccaaggc
1201cgtgctcctc tccctggccg aggaggacta cttgccaact ttcttgtaca aagtggttgg
1261taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa
1321ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)