Construct: ORF TRCN0000469219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018937.3_s317c1
- Derived from:
- ccsbBroadEn_15297
- DNA Barcode:
- CAGATTCATAAGAGAGCCTCTTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KSR2 (283455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538225.3 | 99.8% | 25.5% | (many diffs) |
| 2 | human | 283455 | KSR2 | kinase suppressor of ras 2 | NM_173598.6 | 87.1% | 22.3% | (many diffs) |
| 3 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538224.3 | 86.9% | 22.3% | (many diffs) |
| 4 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019208.2 | 83.8% | 22.6% | (many diffs) |
| 5 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538226.3 | 65.1% | 28.5% | (many diffs) |
| 6 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019210.2 | 61.8% | 6.9% | (many diffs) |
| 7 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_011538229.3 | 52.2% | 34.2% | (many diffs) |
| 8 | human | 283455 | KSR2 | kinase suppressor of ras 2 | XM_017019209.2 | 51.5% | 34.1% | (many diffs) |
| 9 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | NM_001312914.1 | 77.4% | 20.2% | (many diffs) |
| 10 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530393.3 | 77.2% | 20.3% | (many diffs) |
| 11 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530394.3 | 69.5% | 7.6% | (many diffs) |
| 12 | mouse | 333050 | Ksr2 | kinase suppressor of ras 2 | XM_006530395.3 | 61.8% | 8.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggatgaa caggtgtgcg agactgtgga gaaatacgga gccaaccggg 121 aggagtgtgc ccgcctcaac gcctccctct cctgcctcag gaatgtccac atgtcaggag 181 gcaacctttc caaacaagac tggaccatcc agtggcccac cacagagacg gggaaggaga 241 acaatcccgt gtgccccccg gagcccaccc cgtggatccg cacccatctc tcccagagcc 301 ccagggtccc gtccaagtgc gtccagcact attgtcacac cagccccact cccggggccc 361 ctgtgtacac ccacgtggac aggcttaccg tggacgccta cccgggcttg tgcccgcccc 421 cgccactgga gtcgggccac cgttccctgc ccccatcgcc ccggcagcgg cacgcggtcc 481 gcaccccgcc gcgcaccccc aacatcgtca ccaccgtgac cccgccgggc acgccgccca 541 tgaggaagaa gaacaagctg aagcccccgg ggaccccacc gccctcctcc cgaaaactga 601 tacacttgat cccgggattc accgcgctgc atcggagcaa atcccacgag ttccagctgg 661 ggcaccgcgt ggacgaggcc cacacgccca aagccaagaa gagagcaaac ccttgaacct 721 caagatccac agcagcgtag gcagctgcga gaacatcccc tctcagcagc gcttcccgct 781 gctgtccgag cgctccctcc gctccttctt tgtgggacac gcacctttcc tgccttccac 841 ccctcctgtt cacactgagg ccaacttctc tgcaaacaca ctgtcagtgc cacgctggtc 901 cccgcagatc cctcgcagag atctcggcaa ctccatcaag cacaggtttt ccaccaagta 961 ctggatgtct cagacgtgca cagtctgtgg gaaagggatg ctttttggcc tcaagtgtaa 1021 aaactgcaag ttaaagtgcc acaacaaatg caccaaagaa gccccaccct gtcatcttct 1081 gatcatccac cgaggagatc cagcaaggtt agtccggaca gagtccgttc cgtgtgacat 1141 caacaaccct ctacggaagc cacctcgcta ttcagacctg cacatcagtc agacgctccc 1201 caaaaccaac aaaatcaaca aggaccacat ccctgtccct taccagccag actccagcag 1261 caacccctcc tccacgacgt cctccacgcc ctcctcgcca gcaccccccc tccctcctag 1321 tgccacgccg ccttctcccc tacacccttc cccacagtgc acacggcagc agaagaactt 1381 caacctgcca gcatcccact actacaaata caagcagcag ttcatcttcc cagatgtggt 1441 gccggtgccg gagacgccga cccgggcgcc ccaggtcatc ctgcatccgg tgacctcgaa 1501 tccaatcttg gaaggaaatc cattacttca aattgaagtg gagccaacgt cggagaatga 1561 agaggtccat gatgaggccg aagagtcaga ggatgacttc gaggagatga acctgtccct 1621 cctctcggcc cggagcttcc cacgcaaggc cagccagacc agcatcttcc ttcaggagtg 1681 ggacatcccc tttgagcagc tggagatcgg cgagctcatt ggaaagggcc gctttgggca 1741 agtgtaccac ggccgctggc atggcgaggt ggccatccgg ctgattgaca ttgagaggga 1801 caacgaggac cagctcaagg ccttcaagcg ggaggtgatg gcctacaggc agacacggca 1861 tgagaacgtg gtgcttttca tgggtgcctg catgagcccg cctcacctgg cccatcatca 1921 ccagcctctg taagggacgg acgctctatt ccgttgtgag ggatgccaaa atcgttttgg 1981 atgtcaacaa aaccaggcag attgctcaag aaattgtgaa gggcatgggc tacctccacg 2041 ccaagggaat cctacacaag gacctcaagt caaagaacgt cttctatgac aacggcaaag 2101 tggtcatcac ggactttgga ctcttcagca tttctggggt gctgcaggct ggcaggcggg 2161 aggacaaact gcgcatccag aatggctggc tatgccacct ggcaccagag atcatccgcc 2221 agctgtcccc cgacacagag gaggataagc tccCCTTCTC CAAGCACTCT GACGTCTTTG 2281 CCCTTGGCAC AATCTGGTAT GAACTCCACG CCAGGGAATG GCCTTTCAAG ACCCAACCAG 2341 CAGAGGCAAT AATCTGGCAA ATGGGCACAG GCATGAAACC CAACCTCAGC CAGATTGGCA 2401 TGGGAAAAGA AATCTCGGAC ATTCTTCTCT TCTGCTGGGC CTTTGAACAA GAAGAGAGAC 2461 CTACCTTCAC CAAGCTCATG GACATGCTGG AGAAACTGCC AAAGCGAAAC CGTCGCCTGT 2521 CTCACCCTGG ACATTTCTGG AAGTCTGCAG AGCTGTTGCC AACTTTCTTG TACAAAGTGG 2581 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2641 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2701 ACAGATTCAT AAGAGAGCCT CTTCAACGCG TTAAGTCgac aatcaacctc tggattacaa 2761 aatttgtgaa agatt