Transcript: Human XM_017004516.2

PREDICTED: Homo sapiens atypical chemokine receptor 3 (ACKR3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ACKR3 (57007)
Length:
1997
CDS:
105..1193

Additional Resources:

NCBI RefSeq record:
XM_017004516.2
NBCI Gene record:
ACKR3 (57007)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017004516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000363205 CACTATTGGTGTACCTTATAA pLKO_005 1542 3UTR 100% 15.000 21.000 N ACKR3 n/a
2 TRCN0000358300 GGCATAGTGCTGACATATATT pLKO_005 1604 3UTR 100% 15.000 21.000 N ACKR3 n/a
3 TRCN0000363139 GCCGTTCCCTTCTCCATTATC pLKO_005 768 CDS 100% 13.200 10.560 N ACKR3 n/a
4 TRCN0000014512 CGCTCTCCTTCATTTACATTT pLKO.1 241 CDS 100% 13.200 9.240 N ACKR3 n/a
5 TRCN0000378566 TCTTCGTCATCGGCATGATTG pLKO_005 265 CDS 100% 10.800 7.560 N ACKR3 n/a
6 TRCN0000014511 CTTCTCCATTATCGCTGTCTT pLKO.1 776 CDS 100% 4.950 3.465 N ACKR3 n/a
7 TRCN0000014509 CCGGAAGATCATCTTCTCCTA pLKO.1 854 CDS 100% 2.640 1.848 N ACKR3 n/a
8 TRCN0000014510 GCCAGGGAACTTCTCGGACAT pLKO.1 134 CDS 100% 1.350 0.945 N ACKR3 n/a
9 TRCN0000014508 CCCTGCATCCATTCTCTCTTT pLKO.1 1326 3UTR 100% 4.950 2.970 N ACKR3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017004516.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000492025 ATGGTTTAAAAGGTCCACGTGAGA pLX_317 29.7% 99.9% 100% V5 (not translated due to prior stop codon) 162C>T n/a
2 TRCN0000488481 TATTCTCGTATAACACGAGCTGTG pLX_317 29.7% 99.9% 100% V5 (not translated due to prior stop codon) 162C>T n/a
3 TRCN0000489204 AGCGCCGTTGGGCGTACGATATCT pLX_317 29.5% 99.8% 99.7% V5 162C>T;1086_1087insG n/a
4 ccsbBroadEn_08684 pDONR223 100% 99.8% 99.7% None 682A>G;796C>T n/a
5 TRCN0000488560 TTCACACAAAAGCCTCTCCCAGTG pLX_317 29.6% 99.8% 99.7% V5 (not translated due to prior stop codon) 682A>G;796C>T n/a
6 TRCN0000488337 TCCACACTCTTTCTGTCCATTACA pLX_317 26.5% 99.7% 99.4% V5 682A>G;796C>T;1086_1087insG n/a
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