Transcript: Human XM_017015198.1

PREDICTED: Homo sapiens zinc finger CCHC-type containing 7 (ZCCHC7), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZCCHC7 (84186)
Length:
3198
CDS:
782..2317

Additional Resources:

NCBI RefSeq record:
XM_017015198.1
NBCI Gene record:
ZCCHC7 (84186)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017015198.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419048 ATATCCATGGAGATCTCAATT pLKO_005 2465 3UTR 100% 13.200 18.480 N ZCCHC7 n/a
2 TRCN0000428433 TCCGAGAGGTCATGATTATAG pLKO_005 1227 CDS 100% 13.200 18.480 N ZCCHC7 n/a
3 TRCN0000412979 ATTCGGAATCTTCGAGTAGTA pLKO_005 963 CDS 100% 4.950 6.930 N ZCCHC7 n/a
4 TRCN0000033993 CAATCTAATGAGCTGGTTGAT pLKO.1 1151 CDS 100% 4.950 6.930 N ZCCHC7 n/a
5 TRCN0000033991 CGAGATGAGTCATCTAGTGAA pLKO.1 833 CDS 100% 4.950 6.930 N ZCCHC7 n/a
6 TRCN0000033989 GCGGTACTATTCAGCCAACAA pLKO.1 1477 CDS 100% 4.950 6.930 N ZCCHC7 n/a
7 TRCN0000033992 GTCTCCAGTATCTCCATTCAT pLKO.1 1798 CDS 100% 5.625 4.500 N ZCCHC7 n/a
8 TRCN0000414420 ATATTGAGAAGCCTAAATCTG pLKO_005 1188 CDS 100% 4.950 3.960 N ZCCHC7 n/a
9 TRCN0000417576 AGATAGCTAATAACCGAACAC pLKO_005 1440 CDS 100% 4.050 3.240 N ZCCHC7 n/a
10 TRCN0000421935 TACTTTGGAAAGGACAATAAC pLKO_005 2740 3UTR 100% 13.200 9.240 N ZCCHC7 n/a
11 TRCN0000432864 AGAAGCCTTCTAAGCCCTTTC pLKO_005 2151 CDS 100% 6.000 4.200 N ZCCHC7 n/a
12 TRCN0000033990 CCACACGTCAAGAGAAGACAA pLKO.1 2188 CDS 100% 4.950 3.465 N ZCCHC7 n/a
13 TRCN0000429041 GAGGTCATCACTTTGTCTGAT pLKO_005 1049 CDS 100% 4.950 3.465 N ZCCHC7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017015198.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10214 pDONR223 100% 93.9% 93.9% None 1_3delATG;987_988ins96 n/a
2 ccsbBroad304_10214 pLX_304 0% 93.9% 93.9% V5 1_3delATG;987_988ins96 n/a
3 TRCN0000469258 CCCTGCAGGCCGTATCTATACCTG pLX_317 24.9% 93.9% 93.9% V5 1_3delATG;987_988ins96 n/a
4 ccsbBroadEn_12789 pDONR223 100% 60.7% 48.6% None 223A>G;692delC;935_1533del n/a
5 ccsbBroad304_12789 pLX_304 0% 60.7% 48.6% V5 223A>G;692delC;935_1533del n/a
6 TRCN0000472866 AGCCTCCCCAACCATTTCCGTCCC pLX_317 20.9% 60.7% 48.6% V5 223A>G;692delC;935_1533del n/a
7 ccsbBroadEn_16029 pDONR223 0% 39.9% 39.7% None 193T>C;611_612insCCA;616_1533del n/a
8 ccsbBroad304_16029 pLX_304 0% 39.9% 39.7% V5 193T>C;611_612insCCA;616_1533del n/a
9 TRCN0000474870 GTTACCACAAGAGTTATCGGATAC pLX_317 51.5% 39.9% 39.7% V5 193T>C;611_612insCCA;616_1533del n/a
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