Construct: ORF TRCN0000475181
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002312.1_s317c1
- Derived from:
- ccsbBroadEn_15832
- DNA Barcode:
- TTATCTCTCAACAGCCTCAATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF219 (51222)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475181
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51222 | ZNF219 | zinc finger protein 219 | NM_001101672.2 | 99.6% | 99.7% | 681_686delGCCTCA;729C>T |
| 2 | human | 51222 | ZNF219 | zinc finger protein 219 | NM_001102454.1 | 99.6% | 99.7% | 681_686delGCCTCA;729C>T |
| 3 | human | 51222 | ZNF219 | zinc finger protein 219 | NM_016423.3 | 99.6% | 99.7% | 681_686delGCCTCA;729C>T |
| 4 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_017021354.1 | 99.6% | 99.7% | 681_686delGCCTCA;729C>T |
| 5 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_011536825.1 | 94.8% | 94.5% | (many diffs) |
| 6 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_024449623.1 | 93.9% | 94% | 0_1ins123;558_563delGCCTCA;606C>T |
| 7 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_024449624.1 | 93.9% | 94% | 0_1ins123;558_563delGCCTCA;606C>T |
| 8 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_024449625.1 | 93.9% | 94% | 0_1ins123;558_563delGCCTCA;606C>T |
| 9 | human | 51222 | ZNF219 | zinc finger protein 219 | XM_005267739.1 | 93.7% | 93.4% | (many diffs) |
| 10 | mouse | 69890 | Zfp219 | zinc finger protein 219 | NM_001253694.1 | 82.5% | 87.9% | (many diffs) |
| 11 | mouse | 69890 | Zfp219 | zinc finger protein 219 | NM_001253695.1 | 82.5% | 87.9% | (many diffs) |
| 12 | mouse | 69890 | Zfp219 | zinc finger protein 219 | NM_027248.2 | 82.5% | 87.9% | (many diffs) |
| 13 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519498.3 | 82.5% | 87.9% | (many diffs) |
| 14 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519499.3 | 82.5% | 87.9% | (many diffs) |
| 15 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519501.3 | 82.5% | 87.9% | (many diffs) |
| 16 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519502.3 | 82.5% | 87.9% | (many diffs) |
| 17 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519503.2 | 82.5% | 87.9% | (many diffs) |
| 18 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519504.3 | 82.5% | 87.9% | (many diffs) |
| 19 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519505.1 | 82.5% | 87.9% | (many diffs) |
| 20 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_011245176.2 | 82.5% | 87.9% | (many diffs) |
| 21 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_011245177.1 | 82.5% | 87.9% | (many diffs) |
| 22 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_017316180.1 | 82.5% | 87.9% | (many diffs) |
| 23 | mouse | 69890 | Zfp219 | zinc finger protein 219 | NM_001253696.1 | 78.3% | 83.7% | (many diffs) |
| 24 | mouse | 69890 | Zfp219 | zinc finger protein 219 | XM_006519506.3 | 78.3% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2226
- ORF length:
- 2160
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gggctcacgt ccccgcgccc cgagcggcca cttagcgccg tcgccgccgg 121 ctttcgacgg cgagctggat ctgcagcgat actccaacgg gccagccgtg agcgcagggt 181 cgctcgggat gggagcggtg agctggtctg agagtcgtgc aggcgaacgg cgcttcccct 241 gccctgtatg cgggaagcgc ttccgcttca actctatcct tgctttgcac ctgcgggcgc 301 acccaggagc ccaggccttc cagtgccctc actgcggcca ccgcgcggcg cagcgggctc 361 tgctgcgctc gcacctgcgc acacaccagc ccgagcgccc acgtagtcct gctgcacgcc 421 tgttgctgga gttggaagag cgcgcgctac tacgcgaggc ccgactgggg agagcccgaa 481 gctcaggggg catgcaggcc acccctgcca ctgagggtct ggcgcggccc caggctcctt 541 catcgtccgc cttccgttgc ccctactgca aaggcaagtt tcgcacctcg gcggagcgcg 601 aacgccacct gcacatcctg cataggccct ggaagtgcgg cctgtgcagt ttcggctcca 661 gccaggagga ggagctgctg caccacagcc tgacggccca cggggctccc gagcgtcccc 721 tggcggccac ctccgctgcg cctccgcctc agcctcagcc tccaccccag cccgaaccca 781 gatcagttcc ccagccggag ccggagccgg agcccgaacg tgaggcaacc ccgaccccag 841 ctcctgccgc tcccgaggag cccccagcgc ctccggagtt ccgctgccaa gtgtgcggcc 901 agagctttac acagtcttgg tttctcaagg gccacatgcg taagcacaag gcctccttcg 961 atcatgcgtg tccggtgtgc ggccgctgct tcaaggagcc ctggttcctt aagaaccaca 1021 tgaaggtgca cgccagcaag ctgggcccac tgcgtgcccc ggggcctgcc tccgggcctg 1081 cccgcgcccc ccagcctcct gacctcggcc tgctggccta tgagccgttg ggcccagcgc 1141 tcctcttggc cccggctccc accccggccg agcgccgtga gcccccgagc ctcttgggct 1201 acctgagcct gcgagctggc gagggccggc ccaacggcga gggtgctgag cccggtcccg 1261 gccgcagctt cggaggcttc cgcccgctgt cctctgctct cccggcccgg gctcgccggc 1321 accgtgcgga ggagcctgag gaagaagagg aggtggtgga ggccgaggag gaaacctggg 1381 cccggggcag gtcgctgggc tctctggctt ccctgcaccc gcgcccgggt gagggaccgg 1441 ggcactctgc ctctgctgct ggggcccagg caagatcgac cgccacgcag gaagagaatg 1501 ggctgttggt tggagggacc cggcctgaag ggggccgggg cgccaccggc aaggattgtc 1561 ctttctgcgg aaaatctttc cgctcagcac atcacctcaa agtgcatctg cgagtgcaca 1621 caggcgagcg gccctacaag tgtccgcact gcgactacgc gggcacccag tccggctcgc 1681 tcaagtatca cctacagcgc caccaccggg agcagaggag cggggccggc cccgggccac 1741 ccccggagcc accgcctcct tcccagcggg gttcggcccc gcaatctgga gccaagccgt 1801 ctccgcagcc tgcgacctgg gtggagggcg cctcaagtcc ccggcctccT TCTAGCGGTG 1861 CTGGGCCGGG GTCCCGTCGG AAGCCCGCCA GCCCTGGGAG GACCCTGCGC AACGGGCGAG 1921 GCGGTGAGGC CGAACCCCTG GACCTGTCCT TGCGGGCAGG GCCGGGAGGC GAGGCCGGGC 1981 CTGGGGGTGC CCTCCACCGC TGCCTCTTCT GCCCGTTCGC CACTGGAGCC CCAGAGCTCA 2041 TGGCCTTGCA CCTTCAAGTG CACCACAGCC GCCGGGCTAG GGGCCGCCGG CCACCCCAGG 2101 CTGACGCGTC CCCGCCCTAT GCCCGAGTAC CATCAGGAGA GACCCCTCCC AGTCCTTCGC 2161 AGGAAGGGGA GGAGGGCTCC GGGCTGTCCA GACCCGGAGA GGCAGGGCTG GGGGGGCAAG 2221 AACGGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2281 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2341 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTATCTCTC AACAGCCTCA ATAGCACGCG 2401 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt