Transcript: Human XM_017022481.1

PREDICTED: Homo sapiens golgin A6 family like 4 (GOLGA6L4), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GOLGA6L4 (643707)
Length:
3000
CDS:
63..1631

Additional Resources:

NCBI RefSeq record:
XM_017022481.1
NBCI Gene record:
GOLGA6L4 (643707)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022481.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000269219 GGATTCAAGCTCCGCAATAAT pLKO_005 356 CDS 100% 15.000 7.500 Y GOLGA6L9 n/a
2 TRCN0000162932 GCAGTTGGAGCAGCAAGTAAA pLKO.1 1358 CDS 100% 13.200 6.600 Y GOLGA8B n/a
3 TRCN0000269218 CAGTTGGAGCAGCAAGTAAAG pLKO_005 1359 CDS 100% 10.800 5.400 Y GOLGA6L9 n/a
4 TRCN0000150250 GAGAAGAAGCATGAGATACAT pLKO.1 426 CDS 100% 5.625 2.813 Y GOLGA6L5P n/a
5 TRCN0000180631 GAGGTGGAGAAGCTGTTAGAA pLKO.1 1044 CDS 100% 5.625 2.813 Y GOLGA6L9 n/a
6 TRCN0000180938 GAAGAGGTGGAGAAGCTGTTA pLKO.1 1041 CDS 100% 4.950 2.475 Y GOLGA6L5P n/a
7 TRCN0000179455 GAGGAGAAGAAGCATGAGATA pLKO.1 423 CDS 100% 4.950 2.475 Y GOLGA6L5P n/a
8 TRCN0000149653 GAGGAGCTTGTTCAAACTCAA pLKO.1 464 CDS 100% 4.950 2.475 Y GOLGA6L5P n/a
9 TRCN0000153184 GTGGGAGTTCAAACACACAAA pLKO.1 1972 3UTR 100% 4.950 2.475 Y GOLGA8F n/a
10 TRCN0000149152 GCATGAGATACATCTGGTACA pLKO.1 434 CDS 100% 4.050 2.025 Y GOLGA6L5P n/a
11 TRCN0000269223 AGAGGTGGAGAAGCTGTTAAA pLKO_005 1043 CDS 100% 13.200 6.600 Y GOLGA6L9 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022481.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15313 pDONR223 80.2% 72.4% 12.6% None (many diffs) n/a
2 ccsbBroad304_15313 pLX_304 0% 72.4% 12.6% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_13690 pDONR223 100% 52.2% 52.1% None (many diffs) n/a
4 ccsbBroad304_13690 pLX_304 0% 52.2% 52.1% V5 (many diffs) n/a
5 ccsbBroadEn_15348 pDONR223 64.5% 32.9% 29.5% None (many diffs) n/a
6 ccsbBroad304_15348 pLX_304 0% 32.9% 29.5% V5 (many diffs) n/a
7 TRCN0000473379 GCTTCCGCCGAACCGATGGAGCTC pLX_317 100% 21% 20.8% V5 (many diffs) n/a
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