Transcript: Human XM_017022543.2

PREDICTED: Homo sapiens TYRO3 protein tyrosine kinase (TYRO3), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TYRO3 (7301)
Length:
4370
CDS:
21..2336

Additional Resources:

NCBI RefSeq record:
XM_017022543.2
NBCI Gene record:
TYRO3 (7301)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147880 CCTACCTTGAAGGTGAACAG pXPR_003 TGG 655 28% 5 0.6572 TYRO3 TYRO3 76227
2 BRDN0001146050 GGAGTTTGACCATCCACACG pXPR_003 TGG 1735 75% 14 0.1506 TYRO3 TYRO3 76228
3 BRDN0001147403 TGCGCTGTGCCAATGCCTTG pXPR_003 GGG 909 39% 7 -0.3651 TYRO3 TYRO3 76229
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017022543.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000379716 GGGCATCAGCGATGAACTAAA pLKO_005 1514 CDS 100% 13.200 18.480 N TYRO3 n/a
2 TRCN0000231524 CCGGTCCTTCAATCGAGAAAG pLKO_005 1460 CDS 100% 10.800 15.120 N TYRO3 n/a
3 TRCN0000199533 GCCCTCATCCTGCTTCGAAAG pLKO.1 1359 CDS 100% 2.000 2.800 N TYRO3 n/a
4 TRCN0000195673 CCCTGAACCTGTTACCATTGT pLKO.1 515 CDS 100% 4.950 3.960 N TYRO3 n/a
5 TRCN0000381303 TTCAACATCACCGTGACAAAG pLKO_005 705 CDS 100% 10.800 7.560 N TYRO3 n/a
6 TRCN0000002179 GTGGAGAGGAACTACGAAGAT pLKO.1 539 CDS 100% 4.950 3.465 N TYRO3 n/a
7 TRCN0000231525 TCATTGCCTCAAGCGACATTG pLKO_005 1687 CDS 100% 10.800 6.480 N TYRO3 n/a
8 TRCN0000010684 CGCTGAGATTTACAACTACCT pLKO.1 2234 CDS 100% 2.640 1.584 N TYRO3 n/a
9 TRCN0000023526 GCAGCTTGCATGAAGGAGTTT pLKO.1 1725 CDS 100% 4.950 2.475 Y Tyro3 n/a
10 TRCN0000361566 TCATTGCCTCAAGCGACATAG pLKO_005 1687 CDS 100% 10.800 6.480 N Tyro3 n/a
11 TRCN0000264189 CAAGTAGCTGGGACTACAGGA pLKO_005 2949 3UTR 100% 2.640 1.320 Y LINC01098 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017022543.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14871 pDONR223 0% 86.3% 85.8% None (many diffs) n/a
2 ccsbBroad304_14871 pLX_304 0% 86.3% 85.8% V5 (many diffs) n/a
3 TRCN0000479963 TCCTTCCTGGCGGTACGGTCCTAA pLX_317 13.4% 86.3% 85.8% V5 (many diffs) n/a
4 ccsbBroadEn_15617 pDONR223 0% 86.2% 85.8% None (many diffs) n/a
5 ccsbBroad304_15617 pLX_304 0% 86.2% 85.8% V5 (many diffs) n/a
6 ccsbBroadEn_13975 pDONR223 100% 86.2% 85.6% None (many diffs) n/a
7 ccsbBroad304_13975 pLX_304 0% 86.2% 85.6% V5 (not translated due to frame shift) (many diffs) n/a
8 TRCN0000474009 TGCGGAACTACTAATCCGCATTTC pLX_317 9.6% 86.2% 85.6% V5 (not translated due to frame shift) (many diffs) n/a
9 TRCN0000488607 GTCTTAGTGAACTGCAAAAATAAT pLX_317 14.6% 86.1% 85.6% V5 (not translated due to prior stop codon) (many diffs) n/a
10 TRCN0000489876 GCATTCACCTTCAGGTGAGCCTTG pLX_317 15.5% 86.1% 85.5% V5 (many diffs) n/a
11 TRCN0000487789 GGTACCGAAATCCGACTACACCGA pLX_317 21.1% 33.8% 33.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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