Construct: ORF TRCN0000479963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019030.2_s317c1
- Derived from:
- ccsbBroadEn_14871
- DNA Barcode:
- TCCTTCCTGGCGGTACGGTCCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYRO3 (7301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479963
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | NM_006293.4 | 99.9% | 99.7% | 2455C>A;2470A>G |
| 2 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | NM_001330264.1 | 94.8% | 94.7% | 0_1ins135;2320C>A;2335A>G |
| 3 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | XM_017022543.2 | 86.3% | 85.8% | (many diffs) |
| 4 | human | 7302 | TYRO3P | TYRO3P protein tyrosine kin... | NR_028510.1 | 27.6% | (many diffs) | |
| 5 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | NM_019392.2 | 86.8% | 88.7% | (many diffs) |
| 6 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | XM_017317147.1 | 85.2% | 84.9% | (many diffs) |
| 7 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | NM_001290800.1 | 84.4% | 86% | (many diffs) |
| 8 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | XM_006499160.1 | 83.3% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2739
- ORF length:
- 2670
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgctgagg cggagcatgg ggcggccggg gctcccgccg ctgccgctgc 121 cgccgccacc gcggctcggg ctgctgctgg cggctctggc ttctctgctg ctcccggagt 181 ccgccgccgc aggtctgaag ctcatgggag ccccggtgaa gctgacagtg tctcaggggc 241 agccggtgaa gctcaactgc agtgtggagg ggatggagga gcctgacatc cagtgggtga 301 aggatggggc tgtggtccag aacttggacc agttgtacat cccagtcagc gagcagcact 361 ggatcggctt cctcagcctg aagtcagtgg agcgctctga cgccggccgg tactggtgcc 421 aggtggagga tgggggtgaa accgagatct cccagccagt gtggctcacg gtagaaggtg 481 tgccattttt cacagtggag ccaaaagatc tggcagtgcc acccaatgcc cctttccaac 541 tgtcttgtga ggctgtgggt ccccctgaac ctgttaccat tgtctggtgg agaggaacta 601 cgaagatcgg gggacccgct ccctctccat ctgttttaaa tgtaacaggg gtgacccaga 661 gcaccatgtt ttcctgtgaa gctcacaacc taaaaggcct ggcctcttct cgcacagcca 721 ctgttcacct tcaagcactg cctgcagccc ccttcaacat caccgtgaca aagctttcca 781 gcagcaacgc tagtgtggcc tggatgccag gtgctgatgg ccgagctctg ctacagtcct 841 gtacagttca ggtgacacag gccccaggag gctgggaagt cctggctgtt gtggtccctg 901 tgcccccctt tacctgcctg ctccgggacc tggtgcctgc caccaactac agcctcaggg 961 tgcgctgtgc caatgccttg gggccctctc cctatgctga ctgggtgccc tttcagacca 1021 agggtctagc cccagccagc gctccccaaa acctccatgc catccgcaca gattcaggcc 1081 tcatcttgga gtgggaagaa gtgatccccg aggccccttt ggaaggcccc ctgggaccct 1141 acaaactgtc ctgggttcaa gacaatggaa cccaggatga gctgacagtg gaggggacca 1201 gggccaattt gacaggctgg gatccccaaa aggacctgat cgtacgtgtg tgcgtctcca 1261 atgcagttgg ctgtggaccc tggagtcagc cactggtggt ctcttctcat gaccgtgcag 1321 gccagcaggg ccctcctcac agccgcacat cctgggtacc tgtggtcctt ggtgtgctaa 1381 cggccctggt gacggctgct gccctggccc tcatcctgct tcgaaagaga cggaaagaga 1441 cgcggtttgg gcaagccttt gacagtgtca tggcccgggg agagccagcc gttcacttcc 1501 gggcagcccg gtccttcaat cgagaaaggc ccgagcgcat cgaggccaca ttggacagct 1561 tgggcatcag cgatgaacta aaggaaaaac tggaggatgt gctcatccca gagcagcagt 1621 tcaccctggg ccggatgttg ggcaaaggag agtttggttc agtgcgggag gcccagctga 1681 agcaagagga tggctccttt gtgaaagtgg ctgtgaagat gctgaaagct gacatcattg 1741 cctcaagcga cattgaagag ttcctcaggg aagcagcttg catgaaggag tttgaccatc 1801 cacacgtggc caaacttgtt ggggtaagcc tccggagcag ggctaaaggc cgtctcccca 1861 tccccatggt catcttgccc ttcatgaagc atggggacct gcatgccttc ctgctcgcct 1921 cccggattgg ggagaacccc tttaacctac ccctccagac cctgatccgg ttcatggtgg 1981 acattgcctg cggcatggag tacctgagct ctcggaactt catccaccga gacctggctg 2041 ctcggaattg catgctggca gaggacatga cagtgtgtgt ggctgacttc ggactctccc 2101 ggaagatcta cagtggggac tactatcgtc aaggctgtgc ctccaaactg cctgtcaagt 2161 ggctggccct ggagagcctg gccgacaacc tgtatactgt gcagagtgac gtgtgggcgt 2221 tcggggtgac catgtgggag atcatgacac gtgggcagac gccatatgct ggcatcgaaa 2281 acgctgagat ttacaactac ctcattggcg ggaaccgcct gaaacagcct ccggagtgta 2341 tggaggacgt gtatgatctc atgtaccagt gctggagtgc tgacccCAAG CAGCGCCCGA 2401 GCTTTACTTG TCTGCGAATG GAACTGGAGA ACATCTTGGG CCAGCTGTCT GTGCTATCTG 2461 CCAGCCAGGA CCCCTTATAC ATCAACATCG AGAGAGCTGA GGAGCCCACT GCGGGAGGCA 2521 GCATGGAGCT ACCTGGCGGG GATCAGCCCT ACAGTGGGGC TGGGGATGGC AGTGGCATGG 2581 GGGCAGTGGG TGGCACTCCC AGTGACTGTC GGTACATACT CACCCCCGGA GGGCTGGCTG 2641 AGCAGCCAGG GCAGGCAGAG CACCAGCCAG AGAGTCCCCT CAATGAGACA CAGAGGCTTT 2701 TGCTGCTGCA GCAAGGGCTA CTGCCACACA GTAGCTGTTA CCCAACTTTC TTGTACAAAG 2761 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2821 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2881 ACGATCCTTC CTGGCGGTAC GGTCCTAAAC GCGTTAAGTC gacaatcaac ctctggatta 2941 caaaatttgt gaaagatt