Construct: ORF TRCN0000474009
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000891.1_s317c1
- Derived from:
- ccsbBroadEn_13975
- DNA Barcode:
- TGCGGAACTACTAATCCGCATTTC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYRO3 (7301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474009
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | NM_006293.4 | 99.7% | 99.2% | (many diffs) |
| 2 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | NM_001330264.1 | 94.7% | 94.2% | (many diffs) |
| 3 | human | 7301 | TYRO3 | TYRO3 protein tyrosine kinase | XM_017022543.2 | 86.2% | 85.6% | (many diffs) |
| 4 | human | 7302 | TYRO3P | TYRO3P protein tyrosine kin... | NR_028510.1 | 27.5% | (many diffs) | |
| 5 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | NM_019392.2 | 86.7% | 88.3% | (many diffs) |
| 6 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | XM_017317147.1 | 85.1% | 84.5% | (many diffs) |
| 7 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | NM_001290800.1 | 84.3% | 85.6% | (many diffs) |
| 8 | mouse | 22174 | Tyro3 | TYRO3 protein tyrosine kina... | XM_006499160.1 | 83.1% | 84.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2736
- ORF length:
- 2670
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gctgaggcgg agcatggggc ggccggggct cccgccgctg ccgctgccgc 121 cgccactgcg gctcgggctg ctgctggcgg ctctggcttc tctgctgctc ccggagtccg 181 ccgccgcagg tctgaagctc atgggagccc cggtgaagct gacagtgtct caggggcagc 241 cggtgaagct caactgcagt gtggagggga tggaggagcc tgacatccag tgggtgaagg 301 atggggctgt ggtccagaac ttggaccagt tgtacatccc agtcagcgag cagcactgga 361 tcggcttcct cagcctgaag tcagtggagc gctctgacgc cggccggtac tggtgccagg 421 tggaggatgg gggtgaaacc gagatctccc agccagtgtg gctcacggta gaaggtgtgc 481 catttttcac agtggagcca aaagatctgg cagtgccacc caatgcccct ttccaactgt 541 cttgtgaggc tgtgggtccc cctgaacctg ttaccattgt ctggtggaga ggaactacga 601 agatcggggg acccgctccc tctccatctg ttttaaatgt aacaggggtg acccagagca 661 ccatgttttc ctgtgaagct cacaacctaa aaggcctggc ctcttctcgc acagccactg 721 ttcaccttca agcactgcct gcagccccct tcaacatcac cgtgacaaag ctttccagca 781 gcaacgctag tgtggcctgg atgccaggtg ccgatggccg agctctgcta cagtcctgta 841 cagttcaggt gacacaggcc ccaggaggct gggaagtcct ggctgttgtg gtccctgtgc 901 ccccctttac ctgcctgctc cgggacctgg tgcctgccac caactacagc ctcagggtgc 961 gctgtgccaa tgccttgggg ccctctccct atgctgactg ggtgcccttt cagaccaagg 1021 gtctagcccc agccagcgct ccccaaaacc tccatgccat ccgcacagat tcaggcctca 1081 tcttggagtg ggaagaagtg atccccgagg cccctttgga aggccccctg ggaccctaca 1141 aactgtcctg ggttcaagac aatggaaccc aggatgagct gacagtggag gggaccaggg 1201 ccaatttgac aggctgggat ccccaaaagg acctgatcgt acgtgtgtgc gtctccaatg 1261 cagttggctg tggaccctgg agtcagccac tggtggtctc ttctcatgac cgtgcaggcc 1321 agcagggccc tcctcacagc cgcacatcct gggtacctgt ggtccttggt gtgctaacgg 1381 ccctggtgac ggctgctgcc ctggccctca tcctgcttcg aaagagacgg aaagagacgc 1441 ggtttgggca agcctttgac agtgtcatgg cccggggaga gccagccgtt cacttccggg 1501 cagcccggtc cttcaatcga gaaaggcccg agcgcatcga ggccacattg gacagcttgg 1561 gcatcagcga tgaactaaag gaaaaactgg aggatgtgct catcccagag cagcagttca 1621 ccctgggccg gatgttgggc aaaggagagt ttggttcagt gcgggaggcc cagctgaagc 1681 aagaggatag ctcctttgtg aaagtggctg tgaagatgct gaaagctgac atcattgcct 1741 caagcgacat tgaagagttc ctcagggaag cagcttgcat gaaggagttt gaccatccac 1801 acgtggccaa acttgttggg gtaagcctcc ggagcagggc taaaggccgt ctccccatcc 1861 ccatggtcat cttgcccttc atgaagcatg gggacctgca tgccttcctg ctcgcctccc 1921 ggattgggga gaaccccttt aacctacccc tccagaccct gatccggttc atggtggaca 1981 ttgcctgcgg catggagtac ctgagctctc ggaacttcat ccaccgagac ctggctgctc 2041 ggaattgcat gctggcagag gacatgacag tgtgtgtggc tgacttcgga ctctcccgga 2101 agatctacag tggggactac tatcgtcaag gctgtgcctc caaactgcct gtcaagtggc 2161 tggccctgga gagcctggcc gacaacctgt atactgtgca gagtgacgtg tgggcgttcg 2221 gggtgaccat gtgggagatc atgacacgtg ggcagacgcc atatgctggc atcgaaaacg 2281 ctgagattta caactacctc attggcggga accgcctgaa acagcctccg gagtgtatgg 2341 aggacgtgta tgatctcatg taccagtgct ggagtgctga ccccaagcag cgcccgagct 2401 ttacttgtct gcgaatggaa ctggagaaca tcttgggcca gctgtctgtg ctatctgcca 2461 gccaggaccc cttatacatc aacatcGAGA GAGCTGAGGA GCCCACTGCG GGAGGCAGCA 2521 TGGAGCTACC TGGCGGGGAT CAGCCCTACA GTGGGGCTGG GGATGGCAGT GGCATGGGGG 2581 CAGTGGGTGG CACTCCCAGT GACTGTCGGT ACATACTCAC CCCCGGAGGG CTGGCTGAGC 2641 AGCCAGGGCA GGCAGAGCAC CAGCCAGAGA GTCCCCTCAA TGAGACACAG AGGCTTTTGC 2701 TGCTGCAGCA AGGGCTACTG CCACACATAG CTGTTACCCA ACTTTCTTGT ACAAAGTGGT 2761 TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC 2821 TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA 2881 TGCGGAACTA CTAATCCGCA TTTCACGCGT TAAGTCgaca atcaacctct ggattacaaa 2941 atttgtgaaa gatt