Transcript: Human XM_024446381.1

PREDICTED: Homo sapiens cyclin dependent kinase 19 (CDK19), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDK19 (23097)
Length:
6744
CDS:
859..2187

Additional Resources:

NCBI RefSeq record:
XM_024446381.1
NBCI Gene record:
CDK19 (23097)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024446381.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003143 ACCAGCAAATATCCTAGTAAT pLKO.1 1137 CDS 100% 13.200 18.480 N CDK19 n/a
2 TRCN0000197019 GCCAACAGTAGCCTCATAAAG pLKO.1 1543 CDS 100% 13.200 18.480 N CDK19 n/a
3 TRCN0000195069 CGTTCGTATTTATCTAGTTTC pLKO.1 2732 3UTR 100% 10.800 15.120 N CDK19 n/a
4 TRCN0000003140 GCTTGTAGAGAGATTGCACTT pLKO.1 865 CDS 100% 4.050 5.670 N CDK19 n/a
5 TRCN0000352638 GCTTGTAGAGAGATTGCACTT pLKO_005 865 CDS 100% 4.050 5.670 N CDK19 n/a
6 TRCN0000003144 AGGACTGATAGCTCTTCTTTA pLKO.1 2335 3UTR 100% 13.200 9.240 N CDK19 n/a
7 TRCN0000196528 GTATGGCTGCTGTTTGATTAT pLKO.1 955 CDS 100% 13.200 9.240 N CDK19 n/a
8 TRCN0000352698 GTATGGCTGCTGTTTGATTAT pLKO_005 955 CDS 100% 13.200 9.240 N CDK19 n/a
9 TRCN0000003141 GATATTAGAAAGATGCCAGAA pLKO.1 1483 CDS 100% 4.050 2.835 N CDK19 n/a
10 TRCN0000003142 GCGAGAATTGAAGCACCCTAA pLKO.1 888 CDS 100% 4.050 2.835 N CDK19 n/a
11 TRCN0000194732 CTTTCTAAACTGCCTAGTTTG pLKO.1 6408 3UTR 100% 1.080 0.756 N CDK19 n/a
12 TRCN0000196683 GCATGACTTGTGGCATATTAT pLKO.1 981 CDS 100% 15.000 9.000 N CDK19 n/a
13 TRCN0000130146 CAGGTTCAAGTGATTCTCCTA pLKO.1 5011 3UTR 100% 2.640 1.320 Y DICER1-AS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024446381.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489647 ACTGCTTCTTCCAGGGACTCAATC pLX_317 27.9% 88% 88% V5 (not translated due to prior stop codon) 0_1ins180 n/a
2 ccsbBroadEn_15005 pDONR223 100% 80.9% 2.6% None (many diffs) n/a
3 ccsbBroad304_15005 pLX_304 0% 80.9% 2.6% V5 (not translated due to prior stop codon) (many diffs) n/a
4 TRCN0000470685 TTCAGCCAGAAATAAAATGGCCAT pLX_317 25.5% 80.9% 2.6% V5 (not translated due to prior stop codon) (many diffs) n/a
5 ccsbBroad304_14578 pLX_304 50.6% 53.1% 31.2% V5 (not translated due to prior stop codon) (many diffs) n/a
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