Construct: ORF ccsbBroad304_14578
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015528.1_s304c1, BRDN0000459578
- Derived from:
- ccsbBroadEn_14578
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CDK8 (1024)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14578
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001318368.2 | 85.6% | 47.7% | (many diffs) |
| 2 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001260.3 | 85.4% | 47.6% | (many diffs) |
| 3 | human | 23097 | CDK19 | cyclin dependent kinase 19 | NM_015076.5 | 62.2% | 42% | (many diffs) |
| 4 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020317.2 | 58.7% | 20.4% | (many diffs) |
| 5 | human | 23097 | CDK19 | cyclin dependent kinase 19 | NM_001300960.2 | 57.3% | 33.2% | (many diffs) |
| 6 | human | 23097 | CDK19 | cyclin dependent kinase 19 | NM_001300964.2 | 53.1% | 31.2% | (many diffs) |
| 7 | human | 23097 | CDK19 | cyclin dependent kinase 19 | XM_024446377.1 | 53.1% | 31.2% | (many diffs) |
| 8 | human | 23097 | CDK19 | cyclin dependent kinase 19 | XM_024446378.1 | 53.1% | 31.2% | (many diffs) |
| 9 | human | 23097 | CDK19 | cyclin dependent kinase 19 | XM_024446379.1 | 53.1% | 31.2% | (many diffs) |
| 10 | human | 23097 | CDK19 | cyclin dependent kinase 19 | XM_024446380.1 | 53.1% | 31.2% | (many diffs) |
| 11 | human | 23097 | CDK19 | cyclin dependent kinase 19 | XM_024446381.1 | 53.1% | 31.2% | (many diffs) |
| 12 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_017020318.1 | 48.9% | 10.3% | (many diffs) |
| 13 | human | 1024 | CDK8 | cyclin dependent kinase 8 | NM_001346501.2 | 48.7% | 10.3% | (many diffs) |
| 14 | human | 1024 | CDK8 | cyclin dependent kinase 8 | XM_011534865.2 | 48.7% | 10.3% | (many diffs) |
| 15 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359990.1 | 80.9% | 47.5% | (many diffs) |
| 16 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_153599.4 | 80.8% | 47.4% | (many diffs) |
| 17 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504836.4 | 80% | 47.1% | (many diffs) |
| 18 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504838.4 | 78.2% | 44.6% | (many diffs) |
| 19 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_030254515.1 | 77.3% | 52.2% | (many diffs) |
| 20 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504840.4 | 77.2% | 52.1% | (many diffs) |
| 21 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | XM_006504839.4 | 73.7% | 39.6% | (many diffs) |
| 22 | mouse | 264064 | Cdk8 | cyclin-dependent kinase 8 | NM_001359991.1 | 68% | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ctatgacttt aaagtgaagc tgagcagcga gcgggagcgg gtcgaggacc 121 tgtttgaata cgagggctgc aaagttggcc gaggcactta tggtcacgtc tacaaagcca 181 agaggaaaga tgggaaggat gataaagact atgctttaaa acaaatagaa ggaactggga 241 tctctatgtc ggcatgtaga gaaatagcat tacttcgaga gcttaagcat ccaaacgtca 301 tttctcttca aaaggtgttt ctgtctcatg ctgataggaa ggtgtggctt ctgtttgact 361 atgctgaaca tgacctctgg catataatca agtttcacag agcttctaaa gcaaacaaga 421 agccagttca gttacctcgg ggaatggtga agtcactatt atatcagatc ctagatggta 481 ttcactacct gcatgctaac tgggtgttgc acagagattt gaaacctgct aatattttag 541 ttatgggtga aggtcctgag cgaggaagag taaaaattgc tgacatgggc tttgcccgat 601 tatttaattc acctttgaag cctttagcag atttggatcc agtggttgtt acattctggt 661 accgagcccc tgaactactt cttggagcaa ggcattatac caaagctatt gatatttggg 721 cTCTCGACAG CATACAGCAT GAATGCTAAC GCTAATGGAC TCACGTCGAC ATGCCGTCGC 781 AAGGCATCAA CTAGTATCTA TACATGACAG CTGACGAATC AGTATGGATT CTGCAGTAGA 841 TGTAGATTAA AAGATGCCTG ACATCACATA TGAGATTCAG AGAATACGTA TACCAACTGC 901 AGCCTATCAA GTATATGAAA ACATAAAGTT AACCAGATAG TAAAGCATTC ACTTGCTCAG 961 AAGCTGCTTA CCATGACCCA ATAAAGCGAA TACCTCAGAC AGCTATGCAG GACCCCTATT 1021 TCTAGAGACC ATTCTACATC AGACGTTTTT GCCGGTGTCA AATCCTTCCC AAAACGGGAA 1081 TTTTACCGAA GAGGACCTGA TGGCAAAGGG ACCAAAACAG GCGCCACAGG AAGGCCATTA 1141 CCAACATAAA GGAACTGGCC CCCCCGGGAA TCCAGGCCGC CGTTCCCCCC CGGGGCCCCC 1201 GGTGAAGGAA ATGAGAAGTG TTCCTCCTTC CCATACCTTC GGGGGGACTA TCCAGGCCTT 1261 CGGCCATTAG GGGTTCCATT CCCCTGCCGC CTATTCCCAC CCCGGGCCCA GCCCCTTCCC 1321 ACCGCAGAGC CGCCAGGGAT AATTCAGTAC CTTCCCACCG CCTTCCCCAG ACTCCCCTTC 1381 GGCCCATTGG GAATGCCCCA ATTTTTTGGA CAAAAGTGGT TGGTAAGCCT ATCCCTAACC 1441 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAGCTAGCGC TAACCGGTGG CGCGTTAAGT 1501 CGACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT 1561 TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TGTATCATGC TAGCCCCCTG 1621 G