Construct: ORF ccsbBroad304_13547

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016999.1_s304c1, BRDN0000408331
Derived from:
ccsbBroadEn_13547
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC66 (285331)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13547
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285331 CCDC66 coiled-coil domain containi... NM_001141947.3 99.7% 99.7% (many diffs)
2 human 285331 CCDC66 coiled-coil domain containi... NM_001353147.1 99.1% 99.1% (many diffs)
3 human 285331 CCDC66 coiled-coil domain containi... NM_001353148.1 99.1% 99.1% (many diffs)
4 human 285331 CCDC66 coiled-coil domain containi... NM_001353149.1 99% 99% (many diffs)
5 human 285331 CCDC66 coiled-coil domain containi... XM_005265082.4 98.4% 98.4% (many diffs)
6 human 285331 CCDC66 coiled-coil domain containi... NM_001353150.1 97.3% 97.3% (many diffs)
7 human 285331 CCDC66 coiled-coil domain containi... NM_001353151.1 96.2% 96.2% (many diffs)
8 human 285331 CCDC66 coiled-coil domain containi... NM_001012506.5 96.2% 96.2% (many diffs)
9 human 285331 CCDC66 coiled-coil domain containi... NM_001353152.1 96.2% 96.2% (many diffs)
10 human 285331 CCDC66 coiled-coil domain containi... NM_001353153.1 96.2% 96.2% (many diffs)
11 human 285331 CCDC66 coiled-coil domain containi... XM_011533614.2 95.6% 95.6% (many diffs)
12 human 285331 CCDC66 coiled-coil domain containi... XM_011533615.2 95.6% 95.6% (many diffs)
13 human 285331 CCDC66 coiled-coil domain containi... XM_017006230.2 95.6% 95.6% (many diffs)
14 human 285331 CCDC66 coiled-coil domain containi... XM_011533616.3 95.6% 95.6% (many diffs)
15 human 285331 CCDC66 coiled-coil domain containi... XM_011533617.2 95.6% 95.6% (many diffs)
16 human 285331 CCDC66 coiled-coil domain containi... XM_024453462.1 95.6% 95.6% (many diffs)
17 human 285331 CCDC66 coiled-coil domain containi... NM_001353154.1 95.2% 95.3% (many diffs)
18 human 285331 CCDC66 coiled-coil domain containi... XM_024453463.1 94.8% 94.8% (many diffs)
19 human 285331 CCDC66 coiled-coil domain containi... XM_005265083.4 94.6% 94.7% (many diffs)
20 human 285331 CCDC66 coiled-coil domain containi... NM_001353155.1 94.5% 94.6% (many diffs)
21 human 285331 CCDC66 coiled-coil domain containi... NR_148370.1 90% (many diffs)
22 human 285331 CCDC66 coiled-coil domain containi... NR_024460.2 89.1% (many diffs)
23 human 285331 CCDC66 coiled-coil domain containi... NR_148369.1 88.9% (many diffs)
24 human 285331 CCDC66 coiled-coil domain containi... NR_148368.1 88.8% (many diffs)
25 human 285331 CCDC66 coiled-coil domain containi... NR_148377.1 88.2% (many diffs)
26 human 285331 CCDC66 coiled-coil domain containi... NR_148366.1 86.6% (many diffs)
27 human 285331 CCDC66 coiled-coil domain containi... NR_148367.1 86.5% (many diffs)
28 human 285331 CCDC66 coiled-coil domain containi... XR_001740115.2 86.5% (many diffs)
29 human 285331 CCDC66 coiled-coil domain containi... NR_148378.1 85.7% (many diffs)
30 human 285331 CCDC66 coiled-coil domain containi... NR_148375.1 85.6% (many diffs)
31 human 285331 CCDC66 coiled-coil domain containi... NR_148376.1 85.2% (many diffs)
32 human 285331 CCDC66 coiled-coil domain containi... NR_148371.1 84.5% (many diffs)
33 human 285331 CCDC66 coiled-coil domain containi... NR_148372.1 84.1% (many diffs)
34 human 285331 CCDC66 coiled-coil domain containi... NR_148374.1 83.7% (many diffs)
35 human 285331 CCDC66 coiled-coil domain containi... NR_148373.1 83.6% (many diffs)
36 human 285331 CCDC66 coiled-coil domain containi... XM_011533619.2 78.2% 78.2% (many diffs)
37 human 285331 CCDC66 coiled-coil domain containi... XM_024453464.1 78.2% 78.2% (many diffs)
38 human 285331 CCDC66 coiled-coil domain containi... XR_001740111.2 74.3% (many diffs)
39 human 285331 CCDC66 coiled-coil domain containi... NM_001353156.1 67.6% 67% (many diffs)
40 human 285331 CCDC66 coiled-coil domain containi... XM_024453468.1 67.6% 67% (many diffs)
41 human 285331 CCDC66 coiled-coil domain containi... XM_017006236.1 67.1% 66.5% (many diffs)
42 human 285331 CCDC66 coiled-coil domain containi... XM_017006237.2 67.1% 66.5% (many diffs)
43 human 285331 CCDC66 coiled-coil domain containi... XM_017006238.1 67.1% 66.5% (many diffs)
44 human 285331 CCDC66 coiled-coil domain containi... XM_017006239.1 67.1% 66.5% (many diffs)
45 human 285331 CCDC66 coiled-coil domain containi... XM_024453465.1 67.1% 66.5% (many diffs)
46 human 285331 CCDC66 coiled-coil domain containi... XM_024453466.1 67.1% 66.5% (many diffs)
47 human 285331 CCDC66 coiled-coil domain containi... XM_024453467.1 67.1% 66.5% (many diffs)
48 human 285331 CCDC66 coiled-coil domain containi... NM_001353158.1 63.1% 62.6% (many diffs)
49 human 285331 CCDC66 coiled-coil domain containi... XM_017006242.2 63.1% 62.6% (many diffs)
50 human 285331 CCDC66 coiled-coil domain containi... NM_001353160.1 61.5% 61.4% 0_1ins1092;56A>G;721_722insCTT
51 human 285331 CCDC66 coiled-coil domain containi... XM_017006243.2 61.5% 61.4% 0_1ins1092;56A>G;721_722insCTT
52 human 285331 CCDC66 coiled-coil domain containi... XM_024453469.1 61.1% 61% (many diffs)
53 human 285331 CCDC66 coiled-coil domain containi... XM_024453470.1 61.1% 61% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2916
ORF length:
2847
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gaacttggga gatggtttaa agcttgaaac tgaattactg gatggaaaaa
121ccaagctaat attgtctcca tatgaacata aatcaaaaat ttctgtgaag atgggaaata
181aggccaagat tgcaaaatgt cctttaagaa caaaaactgg gcacattcta aaatcaacac
241aagatacttg tattgggagt gaaaaacttt tgcaaaagaa gccagttggt tcagaaacat
301cacaggcaaa aggtgaaaaa aatggaatga ctttttcatc cactaaggat ttatgtaaac
361aatgtataga taaagactgt cttcatatcc agaaagagat ttcacctgca actcctaata
421tgcagaagac tagaaacacc gtaaatacat ctctagtagg taaacagaag cctcacaaaa
481aacacatcac agctgaaaac atgaagagca gtttggtgtg tctaacacaa gaccaactac
541aacagatttt gatgactgta aaccaaggaa atagatctct ttccctgact gagaatggaa
601aggaggcaaa aagtcaatat agtctatatt taaacagtat ttctaatcag ccaaaggatg
661agaacattat gggattattc aaaaaaactg aaatggtttc atctgtccca gctgaaaata
721aatctgtctt aaatgaacat caggagacat ctaaacagtg tgagcaaaaa attgccatag
781agaatgaatg gaaaccagct gatatattca gtactctggg ggaaagggaa tgtgatagaa
841gttcgttgga agcaaaaaaa gcccagtgga ggaaagagct agatgaacag gttgctttaa
901agaagaaaga aaaagaagtt tctgaaaaat ggaatgatcc ttggaaaaaa tctgaaagtg
961ataaaataat atgggaaaaa catcaaattc ttgaccaatc tagggaaaca gtactgctgg
1021agcacccttt cagtgctgtg aaacaagaac tgcagagaaa atggattgaa gagttgaata
1081agcaaataga agatgaccgt caaagaaaaa tagaggaaaa aattatatat tcaaagggtg
1141aggaacatga cagatgggca atgcactttg attcattaaa gagttatcct ggttctcaat
1201ctcagctgtt ctctcggtca acacacaaac aacctgagta cttctgtgtc tctcctgaca
1261ctcaggagct ggctgatgtc agcagtgttt gtacacctac aaccggaagc caggttgaac
1321cttcagagga ggagcatata gcaaaaccta ttaaggatgt ggttatggca aacagtaaga
1381aaacaaactt tctccgttct atgactgctc tcttggaccc agctcagatt gaggaacgag
1441acagacgacg acaaaaacaa ttagagcatc agaaagccat cactgcccag gtagaagaga
1501agcgcaggaa gaaacaactg gaggaagagc aaagaaagaa ggaagaacaa gaagaggagc
1561ttcgcttagc acaggaacgt gaagagatgc agaaacagta tgaagaagac atacttaagc
1621aaaaacaaaa ggaagaaatc atgactctca agacaaatga gctattccag acaatgcagc
1681gagcacagga actggcacag agactaaaac aagaacaaag aatccgagaa ttggcgcaaa
1741agggacatga cacttctaga ctgattaaaa atcttggtgt tgatacaata caaatggaat
1801ataatgcatc taacatttca aattcaagac atgattctga tgaaatcagt ggtaaaatga
1861atacatatat gaattctacg acttcttcta agaaggatac tggtgtgcaa acagatgact
1921taaatatagg aatattcacc aatgcagaat cacattgtgg atcattaatg gagagggaca
1981tcacaaattg ttcatctcct gagatttcgg cagaacttat tggacagttt agcaccaaga
2041aaaacaagca agaactaact caggataaag gagccagctt agaaaaagaa aacaatcggt
2101gtaatgacca gtgtaatcag ttcacaagaa tagagaaaca aacaaaacac atgaagaaat
2161atcctaaaag gcctgattgg aatataaata agccacctaa aaggtatatt ccagcatcag
2221aaaagtaccc taaacagctt caaaagcaga gagaagaaaa aaaagtaagg aggcagatgg
2281aattgcttca tttggtagaa aaaaataatc ctgggcacct ctctcaaaac agaggcattt
2341caccagaaat ttttcattca tctcatcaag aaacggagtc aaagttgagg tggcatctag
2401tcaaaaagga agaagagcct ctgaatattc attcattcag caaggaaagg tctccatcat
2461caccagttcc agtagtgaaa aacagaaccc aacaaactca aaatacatta catttaccac
2521taaaaaacag tagctatgag agagagaatt tgatctcagg aagtaatcaa acagaattat
2581catctgggat ttctgaatca tcccatttta ttccgtatgt tcgaacaaat gagatctatt
2641accttgatcc cgatgcacca ttgtctgggc cttcaaccca ggaccctcag taccaaaatt
2701cacaagactg tggccaaaaa cgacagctat ttgattctga ctgtgtcagg gatccacttc
2761ttaatcctaa catggtgaaa aatagggatc gacagcaagc aatccttaag ggactttcag
2821aactgagaca gggccttctc cagaagcaaa aggagttgga aagtagtctc ctgcctttag
2881ctgaaaatca agaagagagt tttggttctt catttttgcc aactttcttg tacaaagtgg
2941ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
3001ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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