Construct: ORF ccsbBroad304_13548

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001185.1_s304c1, BRDN0000462102
Derived from:
ccsbBroadEn_13548
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CCDC66 (285331)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13548
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285331 CCDC66 coiled-coil domain containi... NM_001012506.5 90.8% 90.7% (many diffs)
2 human 285331 CCDC66 coiled-coil domain containi... NM_001353152.1 90.8% 90.7% (many diffs)
3 human 285331 CCDC66 coiled-coil domain containi... NM_001353153.1 90.8% 90.7% (many diffs)
4 human 285331 CCDC66 coiled-coil domain containi... NM_001353151.1 90.7% 90.6% (many diffs)
5 human 285331 CCDC66 coiled-coil domain containi... XM_011533616.3 90.2% 90.1% (many diffs)
6 human 285331 CCDC66 coiled-coil domain containi... XM_011533617.2 90.2% 90.1% (many diffs)
7 human 285331 CCDC66 coiled-coil domain containi... XM_024453462.1 90.2% 90.1% (many diffs)
8 human 285331 CCDC66 coiled-coil domain containi... XM_011533614.2 90.1% 90% (many diffs)
9 human 285331 CCDC66 coiled-coil domain containi... XM_011533615.2 90.1% 90% (many diffs)
10 human 285331 CCDC66 coiled-coil domain containi... XM_017006230.2 90.1% 90% (many diffs)
11 human 285331 CCDC66 coiled-coil domain containi... XM_024453463.1 89.4% 89.3% (many diffs)
12 human 285331 CCDC66 coiled-coil domain containi... XM_011533619.2 89.2% 89.1% (many diffs)
13 human 285331 CCDC66 coiled-coil domain containi... XM_024453464.1 89.2% 89.1% (many diffs)
14 human 285331 CCDC66 coiled-coil domain containi... NM_001141947.3 87.5% 87.4% (many diffs)
15 human 285331 CCDC66 coiled-coil domain containi... NM_001353147.1 87% 86.8% (many diffs)
16 human 285331 CCDC66 coiled-coil domain containi... NM_001353148.1 86.9% 86.8% (many diffs)
17 human 285331 CCDC66 coiled-coil domain containi... NM_001353149.1 86.8% 86.7% (many diffs)
18 human 285331 CCDC66 coiled-coil domain containi... XM_005265082.4 86.2% 86.1% (many diffs)
19 human 285331 CCDC66 coiled-coil domain containi... NM_001353150.1 85.1% 85% (many diffs)
20 human 285331 CCDC66 coiled-coil domain containi... NM_001353154.1 83% 83% (many diffs)
21 human 285331 CCDC66 coiled-coil domain containi... XM_005265083.4 82.5% 82.4% (many diffs)
22 human 285331 CCDC66 coiled-coil domain containi... NM_001353155.1 82.3% 82.2% (many diffs)
23 human 285331 CCDC66 coiled-coil domain containi... NR_148370.1 79% (many diffs)
24 human 285331 CCDC66 coiled-coil domain containi... NR_024460.2 78% (many diffs)
25 human 285331 CCDC66 coiled-coil domain containi... NR_148369.1 77.9% (many diffs)
26 human 285331 CCDC66 coiled-coil domain containi... NR_148368.1 77.9% (many diffs)
27 human 285331 CCDC66 coiled-coil domain containi... NR_148377.1 77.3% (many diffs)
28 human 285331 CCDC66 coiled-coil domain containi... NM_001353156.1 77.1% 76.3% (many diffs)
29 human 285331 CCDC66 coiled-coil domain containi... XM_024453468.1 77.1% 76.3% (many diffs)
30 human 285331 CCDC66 coiled-coil domain containi... XM_017006236.1 76.5% 75.8% (many diffs)
31 human 285331 CCDC66 coiled-coil domain containi... XM_017006237.2 76.5% 75.8% (many diffs)
32 human 285331 CCDC66 coiled-coil domain containi... XM_017006238.1 76.5% 75.8% (many diffs)
33 human 285331 CCDC66 coiled-coil domain containi... XM_017006239.1 76.5% 75.8% (many diffs)
34 human 285331 CCDC66 coiled-coil domain containi... XM_024453465.1 76.5% 75.8% (many diffs)
35 human 285331 CCDC66 coiled-coil domain containi... XM_024453466.1 76.5% 75.8% (many diffs)
36 human 285331 CCDC66 coiled-coil domain containi... XM_024453467.1 76.5% 75.8% (many diffs)
37 human 285331 CCDC66 coiled-coil domain containi... NR_148375.1 76.4% (many diffs)
38 human 285331 CCDC66 coiled-coil domain containi... NR_148366.1 76% (many diffs)
39 human 285331 CCDC66 coiled-coil domain containi... NR_148376.1 75.9% (many diffs)
40 human 285331 CCDC66 coiled-coil domain containi... NR_148367.1 75.9% (many diffs)
41 human 285331 CCDC66 coiled-coil domain containi... XR_001740115.2 75.8% (many diffs)
42 human 285331 CCDC66 coiled-coil domain containi... NR_148378.1 74.7% (many diffs)
43 human 285331 CCDC66 coiled-coil domain containi... NR_148371.1 74.1% (many diffs)
44 human 285331 CCDC66 coiled-coil domain containi... NR_148372.1 73.7% (many diffs)
45 human 285331 CCDC66 coiled-coil domain containi... NR_148374.1 73.3% (many diffs)
46 human 285331 CCDC66 coiled-coil domain containi... NR_148373.1 73.3% (many diffs)
47 human 285331 CCDC66 coiled-coil domain containi... NM_001353158.1 72% 71.3% (many diffs)
48 human 285331 CCDC66 coiled-coil domain containi... XM_017006242.2 72% 71.3% (many diffs)
49 human 285331 CCDC66 coiled-coil domain containi... NM_001353160.1 70.1% 70% 0_1ins741;56A>G;287G>A
50 human 285331 CCDC66 coiled-coil domain containi... XM_017006243.2 70.1% 70% 0_1ins741;56A>G;287G>A
51 human 285331 CCDC66 coiled-coil domain containi... XM_024453469.1 69.6% 69.5% (many diffs)
52 human 285331 CCDC66 coiled-coil domain containi... XM_024453470.1 69.6% 69.5% (many diffs)
53 human 285331 CCDC66 coiled-coil domain containi... XR_001740111.2 62.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2562
ORF length:
2493
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagaagact agaaacaccg taaatacatc tctagtaggt aaacagaagc
121ctcacaaaaa acacatcaca gctgaaaaca tgaagagcag tttggtgtgt ctaacacaag
181accaactaca acagattttg atgactgtaa accaaggaaa tagatctctt tccctgactg
241agaatggaaa ggaggcaaaa agtcaatata gtctatattt aaacagtatt tctaatcagc
301caaaggatga gaacattatg ggattattca aaaaaactga aatggtttca tctgtcccag
361ctgaaaataa atctgtctta aatgaacatc aggagacatc taaacagtgt gagcaaaaaa
421ttgccataga gaatgaatgg aaaccagctg atatattcag tactctgggg gaaagggaat
481gtgatagaag ttcgttggaa gcaaaaaaag cccagtggag gaaagagcta gatgaacagg
541ttgctttaaa gaagaaagaa aaagaagttt ctgaaaaatg gaatgatcct tggaaaaaat
601ctgaaagtga taaaataata tgggaaaaac atcaaattct tgaccaatct agggaaacag
661tactgctgga gcaccctttc agtgctgtga aacaagaact gcagagaaaa tggattgaag
721agttgaataa gcaaatagaa gatgaccgtc aaagaaaaat agaggaaaaa attatatatt
781caaagggtga ggaacatgac agatgggcaa tgcactttga ttcattaaag agttatcctg
841gttctcaatc tcagctgttc tctcggtcaa cacacaaaca acctgagtac ttctgtgtct
901ctcctgacac tcaggagctg gctgatgtca gcagtgtttg tacacctaca accggaagcc
961aggttgaacc ttcagaggag gagcatatag caaaacctat taaggatgtg gttatggcaa
1021acagtaagaa aacaaacttt ctccgttcta tgactgctct cttggaccca gctcagattg
1081aggaacgaga cagacaacga caaaaacaat tagagcatca gaaagccatc actgcccagg
1141tagaagagaa gcgcaggaag aaacaactgg aggaagagca aagaaagaag gaagaacaag
1201aagaggagct tcgcttagca caggaacgtg aagagatgca gaaacagtat gaagaagaca
1261tacttaagca aaaacaaaag gaagaaatca tgactctcaa gacaaatgag ctattccaga
1321caatgcagcg agcacaggaa ctggcacaga gactaaaaca agaacaaaga atccgagaat
1381tggcgcaaaa gggacatgac acttctagac tgattaaaaa tcttggtgtt gatacaatac
1441aaatggaata taatgcatct aacatttcaa attcaagaca tgattctgat gaaatcagtg
1501gtaaaatgaa tacatatatg aattctacga cttctaagaa ggatactggt gtgcaaacag
1561atgacttaaa tataggaata ttcaccaatg cagaatcaca ttgtggatca ttaatggaga
1621gggacatcac aaattgttca tctcctgaga tttcggcaga acttattgga cagtttagca
1681ccaagaaaaa caagcaagaa ctaactcagg ataaaggagc cagcttagaa aaagaaaaca
1741atcggtgtaa tgaccagtgt aatcagttca caagaataga gaaacaaaca aaacacatga
1801agaaatatcc taaaaggcct gattggaata taaataagcc acctaaaagg tatattccag
1861catcagaaaa gtaccctaaa cagcttcaaa agcagagaga agaaaaaaaa gtaaggaggc
1921agatggaatt gcttcatttg gtagaaaaaa ataatcctgg gcacctctct caaaacagag
1981gcatttcacc agaaattttt cattcatctc atcaagaaac ggagtcaaag ttgaggtggc
2041atctagtcaa aaaggaagaa gagcctctga atattcattc attcagcaag gaaaggtctc
2101catcatcacc agttccagta gtgaaaaaca gaacccaaca aactcaaaat acattacatt
2161taccactaaa aaacagtagc tatgagagag agaatttgat ctcaggaagt aatcaaacag
2221aattatcatc tgggatttct gaatcatccc attttattcc gtatgttcga acaaatgaga
2281tctattacct tgatcccgat gcaccattgt ctgggccttc aacccaggac cctcagtacc
2341aaaattcaca agactgtggc caaaaacgac agctatttga ttctgactgt gtcagggatc
2401cacttcttaa tcctaacatg gtgaaaaata gggatcgaca gcaagcaatc cttaagggac
2461tttcagaact gagacagggc cttctccaga agcaaaagga gttggaaagt agtctcctgc
2521ctttagctga aaatcaagaa gagagttttg gttcttcatt tttgccaact ttcttgtaca
2581aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2641ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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