Transcript: Human XR_002959355.1

PREDICTED: Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant X11, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CFLAR (8837)
Length:
2434
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_002959355.1
NBCI Gene record:
CFLAR (8837)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_002959355.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000364098 CCAGATCAACTGGATTTATTA pLKO_005 473 3UTR 100% 15.000 21.000 N CFLAR n/a
2 TRCN0000320671 ATCTCAGTATGCATGGTATAT pLKO_005 830 3UTR 100% 13.200 18.480 N CFLAR n/a
3 TRCN0000320749 TTGGTGAGGATTTGGATAAAT pLKO_005 336 3UTR 100% 15.000 10.500 N CFLAR n/a
4 TRCN0000378209 ACATGGGCCGAGGCAAGATAA pLKO_005 393 3UTR 100% 13.200 9.240 N CFLAR n/a
5 TRCN0000007231 GCATCACATCAGGAGGATGTT pLKO.1 981 3UTR 100% 0.495 0.347 N CFLAR n/a
6 TRCN0000007232 CACTCTGAGAAAGAAACTTAT pLKO.1 1389 3UTR 100% 13.200 7.920 N CFLAR n/a
7 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 1455 3UTR 100% 4.950 2.475 Y CFLAR n/a
8 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 1455 3UTR 100% 4.950 2.475 Y C19orf31 n/a
9 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 1453 3UTR 100% 4.950 2.475 Y ERN2 n/a
10 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 1453 3UTR 100% 4.950 2.475 Y P3H4 n/a
11 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 1453 3UTR 100% 4.950 2.475 Y P3H4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_002959355.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02030 pDONR223 100% 47.7% None (many diffs) n/a
2 ccsbBroad304_02030 pLX_304 0% 47.7% V5 (many diffs) n/a
3 TRCN0000466010 GCCCTAAGGACAATAGACCAGTAT pLX_317 22.8% 47.7% V5 (many diffs) n/a
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