Construct: ORF ccsbBroadEn_02030

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF012884.1_s300c1, BRDN0000396766
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CFLAR (8837)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001127183.4	100%	100%
2	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_003879.7	100%	100%
3	human	8837	CFLAR	CASP8 and FADD like apoptos...	XM_017005190.2	100%	100%
4	human	8837	CFLAR	CASP8 and FADD like apoptos...	XM_017005191.2	100%	100%
5	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001308042.3	93.7%	87.9%	(many diffs)
6	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001351590.2	93.7%	87.9%	(many diffs)
7	human	8837	CFLAR	CASP8 and FADD like apoptos...	XM_017005193.2	93.7%	87.9%	(many diffs)
8	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001202516.3	92.7%	92.7%	605_606ins105
9	human	8837	CFLAR	CASP8 and FADD like apoptos...	XM_017005189.2	91.5%	86%	(many diffs)
10	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001202517.3	80%	80%	0_1ins288
11	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001351593.2	80%	80%	0_1ins288
12	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001351594.2	80%	80%	0_1ins288
13	human	8837	CFLAR	CASP8 and FADD like apoptos...	XM_017005195.2	80%	80%	0_1ins288
14	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001202518.2	73.8%	68.7%	(many diffs)
15	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001202519.3	73.8%	68.7%	(many diffs)
16	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001202515.1	48.9%	48.9%	0_1ins735
17	human	8837	CFLAR	CASP8 and FADD like apoptos...	XR_002959355.1	47.7%		(many diffs)
18	human	8837	CFLAR	CASP8 and FADD like apoptos...	XR_002959354.1	46.8%		1_445del;967_968ins83;1803_2814del
19	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001308043.2	46.6%	41.6%	(many diffs)
20	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001127184.3	43.9%	42.5%	(many diffs)
21	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001351591.1	43.9%	42.5%	(many diffs)
22	human	8837	CFLAR	CASP8 and FADD like apoptos...	NM_001351592.1	43.9%	42.5%	(many diffs)
23	human	8837	CFLAR	CASP8 and FADD like apoptos...	XR_001739013.2	43.6%		1_453del;1756_2659del;2798_3300del
24	human	8837	CFLAR	CASP8 and FADD like apoptos...	XR_001739016.2	31.5%		1_2473del;3265_3535del;4185_4561del
25	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147245.2	19%		1_455del;1896_7540del
26	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147248.2	17.6%		(many diffs)
27	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147253.2	17.4%		1_323del;1626_2464del;2603_8247del
28	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147247.2	17.2%		1_455del;1758_2596del;2735_8347del
29	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147251.2	17.1%		1_455del;1758_2596del;2735_8379del
30	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147242.2	17%		(many diffs)
31	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147249.2	16.1%		(many diffs)
32	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147246.2	15.5%		(many diffs)
33	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147243.2	13.8%		(many diffs)
34	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147244.2	13.8%		(many diffs)
35	human	8837	CFLAR	CASP8 and FADD like apoptos...	NR_147250.2	11.8%		(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1506

ORF length:

1440

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGTC TGCTGAAGTC ATCCATCAGG TTGAAGAAGC ACTTGATACA GATGAGAAGG
121	AGATGCTGCT CTTTTTGTGC CGGGATGTTG CTATAGATGT GGTTCCACCT AATGTCAGGG
181	ACCTTCTGGA TATTTTACGG GAAAGAGGTA AGCTGTCTGT CGGGGACTTG GCTGAACTGC
241	TCTACAGAGT GAGGCGATTT GACCTGCTCA AACGTATCTT GAAGATGGAC AGAAAAGCTG
301	TGGAGACCCA CCTGCTCAGG AACCCTCACC TTGTTTCGGA CTATAGAGTG CTGATGGCAG
361	AGATTGGTGA GGATTTGGAT AAATCTGATG TGTCCTCATT AATTTTCCTC ATGAAGGATT
421	ACATGGGCCG AGGCAAGATA AGCAAGGAGA AGAGTTTCTT GGACCTTGTG GTTGAGTTGG
481	AGAAACTAAA TCTGGTTGCC CCAGATCAAC TGGATTTATT AGAAAAATGC CTAAAGAACA
541	TCCACAGAAT AGACCTGAAG ACAAAAATCC AGAAGTACAA GCAGTCTGTT CAAGGAGCAG
601	GGACAAGTTA CAGGAATGTT CTCCAAGCAG CAATCCAAAA GAGTCTCAAG GATCCTTCAA
661	ATAACTTCAG GCTCCATAAT GGGAGAAGTA AAGAACAAAG ACTTAAGGAA CAGCTTGGCG
721	CTCAACAAGA ACCAGTGAAG AAATCCATTC AGGAATCAGA AGCTTTTTTG CCTCAGAGCA
781	TACCTGAAGA GAGATACAAG ATGAAGAGCA AGCCCCTAGG AATCTGCCTG ATAATCGATT
841	GCATTGGCAA TGAGACAGAG CTTCTTCGAG ACACCTTCAC TTCCCTGGGC TATGAAGTCC
901	AGAAATTCTT GCATCTCAGT ATGCATGGTA TATCCCAGAT TCTTGGCCAA TTTGCCTGTA
961	TGCCCGAGCA CCGAGACTAC GACAGCTTTG TGTGTGTCCT GGTGAGCCGA GGAGGCTCCC
1021	AGAGTGTGTA TGGTGTGGAT CAGACTCACT CAGGGCTCCC CCTGCATCAC ATCAGGAGGA
1081	TGTTCATGGG AGATTCATGC CCTTATCTAG CAGGGAAGCC AAAGATGTTT TTTATTCAGA
1141	ACTATGTGGT GTCAGAGGGC CAGCTGGAGG ACAGCAGCCT CTTGGAGGTG GATGGGCCAG
1201	CGATGAAGAA TGTGGAATTC AAGGCTCAGA AGCGAGGGCT GTGCACAGTT CACCGAGAAG
1261	CTGACTTCTT CTGGAGCCTG TGTACTGCGG ACATGTCCCT GCTGGAGCAG TCTCACAGCT
1321	CACCATCCCT GTACCTGCAG TGCCTCTCCC AGAAACTGAG ACAAGAAAGA AAACGCCCAC
1381	TCCTGGATCT TCACATTGAA CTCAATGGCT ACATGTATGA TTGGAACAGC AGAGTTTCTG
1441	CCAAGGAGAA ATATTATGTC TGGCTGCAGC ACACTCTGAG AAAGAAACTT ATCCTCTCCT
1501	ACACATACCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt
1561	gttgcaacga ac

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