Construct: ORF ccsbBroad304_02030

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012884.1_s304c1, BRDN0000410329
Derived from:
ccsbBroadEn_02030
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CFLAR (8837)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02030
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001127183.4 100% 100%
2 human 8837 CFLAR CASP8 and FADD like apoptos... NM_003879.7 100% 100%
3 human 8837 CFLAR CASP8 and FADD like apoptos... XM_017005190.2 100% 100%
4 human 8837 CFLAR CASP8 and FADD like apoptos... XM_017005191.2 100% 100%
5 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001308042.3 93.7% 87.9% (many diffs)
6 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001351590.2 93.7% 87.9% (many diffs)
7 human 8837 CFLAR CASP8 and FADD like apoptos... XM_017005193.2 93.7% 87.9% (many diffs)
8 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001202516.3 92.7% 92.7% 605_606ins105
9 human 8837 CFLAR CASP8 and FADD like apoptos... XM_017005189.2 91.5% 86% (many diffs)
10 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001202517.3 80% 80% 0_1ins288
11 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001351593.2 80% 80% 0_1ins288
12 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001351594.2 80% 80% 0_1ins288
13 human 8837 CFLAR CASP8 and FADD like apoptos... XM_017005195.2 80% 80% 0_1ins288
14 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001202518.2 73.8% 68.7% (many diffs)
15 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001202519.3 73.8% 68.7% (many diffs)
16 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001202515.1 48.9% 48.9% 0_1ins735
17 human 8837 CFLAR CASP8 and FADD like apoptos... XR_002959355.1 47.7% (many diffs)
18 human 8837 CFLAR CASP8 and FADD like apoptos... XR_002959354.1 46.8% 1_445del;967_968ins83;1803_2814del
19 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001308043.2 46.6% 41.6% (many diffs)
20 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001127184.3 43.9% 42.5% (many diffs)
21 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001351591.1 43.9% 42.5% (many diffs)
22 human 8837 CFLAR CASP8 and FADD like apoptos... NM_001351592.1 43.9% 42.5% (many diffs)
23 human 8837 CFLAR CASP8 and FADD like apoptos... XR_001739013.2 43.6% 1_453del;1756_2659del;2798_3300del
24 human 8837 CFLAR CASP8 and FADD like apoptos... XR_001739016.2 31.5% 1_2473del;3265_3535del;4185_4561del
25 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147245.2 19% 1_455del;1896_7540del
26 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147248.2 17.6% (many diffs)
27 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147253.2 17.4% 1_323del;1626_2464del;2603_8247del
28 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147247.2 17.2% 1_455del;1758_2596del;2735_8347del
29 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147251.2 17.1% 1_455del;1758_2596del;2735_8379del
30 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147242.2 17% (many diffs)
31 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147249.2 16.1% (many diffs)
32 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147246.2 15.5% (many diffs)
33 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147243.2 13.8% (many diffs)
34 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147244.2 13.8% (many diffs)
35 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147250.2 11.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1506
ORF length:
1440
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tgctgaagtc atccatcagg ttgaagaagc acttgataca gatgagaagg
121agatgctgct ctttttgtgc cgggatgttg ctatagatgt ggttccacct aatgtcaggg
181accttctgga tattttacgg gaaagaggta agctgtctgt cggggacttg gctgaactgc
241tctacagagt gaggcgattt gacctgctca aacgtatctt gaagatggac agaaaagctg
301tggagaccca cctgctcagg aaccctcacc ttgtttcgga ctatagagtg ctgatggcag
361agattggtga ggatttggat aaatctgatg tgtcctcatt aattttcctc atgaaggatt
421acatgggccg aggcaagata agcaaggaga agagtttctt ggaccttgtg gttgagttgg
481agaaactaaa tctggttgcc ccagatcaac tggatttatt agaaaaatgc ctaaagaaca
541tccacagaat agacctgaag acaaaaatcc agaagtacaa gcagtctgtt caaggagcag
601ggacaagtta caggaatgtt ctccaagcag caatccaaaa gagtctcaag gatccttcaa
661ataacttcag gctccataat gggagaagta aagaacaaag acttaaggaa cagcttggcg
721ctcaacaaga accagtgaag aaatccattc aggaatcaga agcttttttg cctcagagca
781tacctgaaga gagatacaag atgaagagca agcccctagg aatctgcctg ataatcgatt
841gcattggcaa tgagacagag cttcttcgag acaccttcac ttccctgggc tatgaagtcc
901agaaattctt gcatctcagt atgcatggta tatcccagat tcttggccaa tttgcctgta
961tgcccgagca ccgagactac gacagctttg tgtgtgtcct ggtgagccga ggaggctccc
1021agagtgtgta tggtgtggat cagactcact cagggctccc cctgcatcac atcaggagga
1081tgttcatggg agattcatgc ccttatctag cagggaagcc aaagatgttt tttattcaga
1141actatgtggt gtcagagggc cagctggagg acagcagcct cttggaggtg gatgggccag
1201cgatgaagaa tgtggaattc aaggctcaga agcgagggct gtgcacagtt caccgagaag
1261ctgacttctt ctggagcctg tgtactgcgg acatgtccct gctggagcag tctcacagct
1321caccatccct gtacctgcag tgcctctccc agaaactgag acaagaaaga aaacgcccac
1381tcctggatct tcacattgaa ctcaatggct acatgtatga ttggaacagc agagtttctg
1441ccaaggagaa atattatgtc tggctgcagc acactctgag aaagaaactt atcctctcct
1501acacataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1561gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1621acctctggat ta

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