Construct: ORF TRCN0000465262
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003664.1_s317c1
- Derived from:
- ccsbBroadEn_15774
- DNA Barcode:
- GATGGAACCCTTTCTCTATATGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- INTS7 (25896)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465262
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199811.1 | 99.9% | 99.8% | 1274A>G |
| 2 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199812.1 | 98.6% | 97.8% | (many diffs) |
| 3 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_015434.4 | 98.5% | 98.4% | 1274A>G;2274_2315del |
| 4 | human | 25896 | INTS7 | integrator complex subunit 7 | XM_011509396.2 | 94.9% | 94.8% | 1274A>G;2274_2423del |
| 5 | human | 25896 | INTS7 | integrator complex subunit 7 | NM_001199809.1 | 93.4% | 93.3% | 223_224ins147;1127A>G;2127_2168del |
| 6 | human | 25896 | INTS7 | integrator complex subunit 7 | NR_037667.1 | 61.9% | (many diffs) | |
| 7 | human | 25896 | INTS7 | integrator complex subunit 7 | XM_017000962.1 | 53.9% | 51.7% | (many diffs) |
| 8 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_178632.6 | 84.8% | 93.4% | (many diffs) |
| 9 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_001306203.1 | 73.7% | 80.7% | (many diffs) |
| 10 | mouse | 77065 | Ints7 | integrator complex subunit 7 | NM_001306204.1 | 73.7% | 80.7% | (many diffs) |
| 11 | mouse | 77065 | Ints7 | integrator complex subunit 7 | XR_865812.3 | 47.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2910
- ORF length:
- 2844
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcaaactca actaagtctt tcctggcaga tgccggctat ggcgaacagg 121 aactggatgc caactctgcc cttatggaat tggacaaagg cctaagatct ggcaaacttg 181 gtgaacagtg tgaagcagtt gttcgctttc ccagactttt tcagaagtat ccattcccta 241 ttcttatcaa ttctgcattc ctaaagttag ctgatgtttt cagagttgga aataatttcc 301 tgaggctatg tgttcttaaa gttacccaac aaagtgagaa acatttggag aagattctaa 361 atgtggatga atttgtgaag agaatttttt ctgtgattca tagtaatgat cctgtggcaa 421 gagccatcac cctccggatg ttgggaagtc tggcatcaat aattcctgag aggaagaatg 481 ctcatcatag tattcgtcag agtttagatt cacatgataa tgtagaagtt gaagctgctg 541 tttttgctgc tgcaaacttc tctgcacagt caaaggattt tgctgtagga atctgtaaca 601 aaatcagtga aatgattcaa ggtttagcga caccagtaga cttgaagcta aaattgatac 661 ccattctaca gcacatgcac catgatgcaa tcttggcttc cagtgctcgt cagcttttac 721 aacagctggt cacatcctat ccgtccacca aaatggtgat tgtgtctttg cacactttca 781 ctctgcttgc agcgtcatct ttggttgata cacctaagca gattcagctt ctgttgcagt 841 atttgaagaa tgatcccagg aaggcagtaa agagacttgc tattcaagat ctgaaattac 901 ttgctaataa aacaccacat acttggagta gggagaatat tcaggcactt tgtgagtgtg 961 ccctccagac tccttatgac agcttaaaac tagggatgtt gtctgtcctt tccacactat 1021 cagggaccat cgccatcaaa cattacttca gtatagttcc aggaaatgtg agttcttctc 1081 ccagatcttc tgatttagtc aaattagccc aagagtgctg ttaccataat aacaggggca 1141 ttgcagctca tggagttaga gtcctaacta atataactgt ttcttgtcaa gaaaaggatc 1201 ttttggcact ggaacaagat gctgtctttg gcctggaatc cctactggta ctttgtagtc 1261 aagatgatag tccaggtgct caggccactt taaagattgc tctaaactgt atggtgaagt 1321 tggccaaggg caggccccgt cttagccagt cagtagttga gaccttgttg actcaattgc 1381 acagtgctca agacgctgcc cggattttga tgtgccattg cctggcagcc attgccatgc 1441 aactgccggt gctgggtgat gggatgcttg gtgacctcat ggagctgtac aaggtgattg 1501 gacgatcagc cacagacaag caacaagaac ttctggtgag tttggctact gtgatttttg 1561 ttgcaagtca gaaggcattg tctgtggaaa gtaaggcagt aattaagcag cagcttgaaa 1621 gtgtctccaa tggatggact gtataccgta ttgccagaca ggcttccaga atgggtaatc 1681 atgacatggc caaagagctt tatcagagtt tgctgactca ggttgcctca gaacatttct 1741 acttctggct aaatagtttg aaggagtttt cacatgcaga acagtgtctc actgggttgc 1801 aagaggaaaa ttatagttca gcactttctt gcattgctga atctttaaaa ttctatcaca 1861 aagggattgc ttccttaaca gcagctagta caccactgaa tcctttaagc tttcagtgtg 1921 aatttgtaaa actcaggatt gaccttttac aagccttctc tcaacttatc tgtacttgta 1981 atagcctgaa gacaagccca ccacctgcaa ttgccacaac aattgccatg accttaggaa 2041 atgacctcca gaggtgtggt cgcatctcca atcagatgaa acagtccatg gaagaatttc 2101 gaagccttgc ttctcgatat ggagatcttt accaggcatc ttttgatgct gactcagcaa 2161 ctttgaggaa tgttgaacta cagcagcaga gctgtttact gatatctcat gcaatagaag 2221 ccctgatttt ggatccagaa tcagcaagtt tccaggaata tggatctact ggaacagccc 2281 atgctgatag tgaatatgaa agaagaatga tgtctgtata taatcatgtc ttggaggagc 2341 acacagcatg cctctgcaat gccatcattg ctttgctgaa agttcccctt tctttccaga 2401 gatatttttt ccagaaacta cagtctacca gcatcaagct tgctctgtca ccatcgcccc 2461 ggaatcctgc agagcccatt gctgtccaga ataaccagca gctggcgcta aaggtagagg 2521 gagtggttca gcacggatct aaaccaggac tcttccgcaa aattcagtct gtctgtctga 2581 atgtttcttc cacactgcag agtaaatctg gacaagacta caagataccc attgacaaca 2641 tgacCAATGA GATGGAGCAA AGGGTTGAAC CTCATAATGA TTACTTCAGT ACTCAATTTC 2701 TGTTGAACTT TGCTATCCTT GGAACACACA ACATTACAGT GGAATCTTCT GTGAAAGATG 2761 CCAATGGTAT AGTATGGAAG ACTGGTCCCA GAACTACCAT ATTTGTAAAA TCCCTGGAAG 2821 ACCCTTATTC CCAGCAAATT CGCTTACAAC AGCAGCAAGC CCAGCAGCCA TTACAGCAGC 2881 AGCAGCAACG CAATGCCTAC ACACGGTTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2941 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3001 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGATGG 3061 AACCCTTTCT CTATATGGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3121 tgaaagatt