Construct: ORF TRCN0000465408

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006321.1_s317c1
Derived from:
ccsbBroadEn_15304
DNA Barcode:
CACCCATAGAAGAATTACCGCCGA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PKD1P1 (339044)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465408
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 729978 NPIPB2 nuclear pore complex intera... XM_024450429.1 33.1% 31.1% (many diffs)
2 human 729978 NPIPB2 nuclear pore complex intera... NM_001355514.1 29.5% 27.8% (many diffs)
3 human 729978 NPIPB2 nuclear pore complex intera... XM_024450428.1 29.5% 27.8% (many diffs)
4 human 729978 NPIPB2 nuclear pore complex intera... XM_024450431.1 29.5% 27.8% (many diffs)
5 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022836.2 27.6% 27.2% 1_279del;482C>A;485_738del
6 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022840.2 27.6% 27.2% 1_279del;482C>A;485_738del
7 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030214.2 27.3% 26.4% (many diffs)
8 human 728741 NPIPB6 nuclear pore complex intera... XM_005255743.3 24.8% 22.6% (many diffs)
9 human 440348 NPIPB15 nuclear pore complex intera... XM_011523501.2 24.2% 21.9% (many diffs)
10 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022827.2 20.4% 18.5% (many diffs)
11 human 729978 NPIPB2 nuclear pore complex intera... XM_024450424.1 20.3% 19% (many diffs)
12 human 729978 NPIPB2 nuclear pore complex intera... XM_024450425.1 20.3% 19% (many diffs)
13 human 729978 NPIPB2 nuclear pore complex intera... XM_024450426.1 20.3% 19% (many diffs)
14 human 100288332 NPIPA5 nuclear pore complex intera... NM_001277325.2 19.2% 18.5% (many diffs)
15 human 9284 NPIPA1 nuclear pore complex intera... NM_006985.4 19.1% 18.5% (many diffs)
16 human 642778 NPIPA3 nuclear pore complex intera... NM_001277323.1 18.9% 18.2% (many diffs)
17 human 642799 NPIPA2 nuclear pore complex intera... NM_001277324.1 18.9% 18.2% (many diffs)
18 human 642778 NPIPA3 nuclear pore complex intera... XM_011522580.2 18.7% 18% (many diffs)
19 human 642799 NPIPA2 nuclear pore complex intera... XM_024450388.1 18.7% 18% (many diffs)
20 human 642799 NPIPA2 nuclear pore complex intera... XM_024450387.1 18.6% 17.6% (many diffs)
21 human 101059938 NPIPA7 nuclear pore complex intera... NM_001282507.1 18.4% 18.1% 1_648del;851C>A;854_1107del
22 human 101059953 NPIPA8 nuclear pore complex intera... NM_001282511.2 18.4% 18.1% 1_648del;851C>A;854_1107del
23 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022834.1 18.4% 18.1% 1_648del;851C>A;854_1107del
24 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022835.1 18.4% 18.1% 1_648del;851C>A;854_1107del
25 human 728741 NPIPB6 nuclear pore complex intera... XM_017023631.2 18.4% 16.7% (many diffs)
26 human 100288332 NPIPA5 nuclear pore complex intera... XM_024450132.1 18.2% 17.6% (many diffs)
27 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452519.1 18.2% 17.6% (many diffs)
28 human 101059938 NPIPA7 nuclear pore complex intera... XM_011522339.3 18.2% 17.9% 1_660del;863C>A;866_1119del
29 human 728734 NPIPB8 nuclear pore complex intera... XM_024450418.1 18% 16.4% (many diffs)
30 human 642778 NPIPA3 nuclear pore complex intera... XM_017023554.1 17.9% 17.3% (many diffs)
31 human 642778 NPIPA3 nuclear pore complex intera... XM_024450379.1 17.9% 17.3% (many diffs)
32 human 642799 NPIPA2 nuclear pore complex intera... XM_024450385.1 17.9% 17.3% (many diffs)
33 human 642799 NPIPA2 nuclear pore complex intera... XM_024450386.1 17.9% 17.3% (many diffs)
34 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022833.2 17.8% 17.5% 1_684del;887C>A;890_1143del
35 human 642778 NPIPA3 nuclear pore complex intera... XM_011522578.2 17.5% 16.9% (many diffs)
36 human 642799 NPIPA2 nuclear pore complex intera... XM_024450384.1 17.5% 16.9% (many diffs)
37 human 101059938 NPIPA7 nuclear pore complex intera... XM_005255735.5 17.1% 16.9% 1_729del;932C>A;935_1188del
38 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030211.2 17% 16.4% (many diffs)
39 human 642778 NPIPA3 nuclear pore complex intera... XM_011522576.2 16.6% 16% (many diffs)
40 human 642778 NPIPA3 nuclear pore complex intera... XM_011522577.2 16.6% 16% (many diffs)
41 human 642778 NPIPA3 nuclear pore complex intera... XM_017023553.1 16.6% 16% (many diffs)
42 human 642799 NPIPA2 nuclear pore complex intera... XM_024450381.1 16.6% 16% (many diffs)
43 human 642799 NPIPA2 nuclear pore complex intera... XM_024450382.1 16.6% 16% (many diffs)
44 human 642799 NPIPA2 nuclear pore complex intera... XM_024450383.1 16.6% 16% (many diffs)
45 human 440348 NPIPB15 nuclear pore complex intera... XM_024450290.1 16.4% 14.9% (many diffs)
46 human 728741 NPIPB6 nuclear pore complex intera... XM_011545931.2 16.1% 14.7% (many diffs)
47 human 728734 NPIPB8 nuclear pore complex intera... XM_024450417.1 15.8% 14.4% (many diffs)
48 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022826.2 15.8% 14.4% (many diffs)
49 human 728741 NPIPB6 nuclear pore complex intera... XM_005255741.4 15.6% 14.2% (many diffs)
50 human 728741 NPIPB6 nuclear pore complex intera... XM_017023630.1 15.6% 14.2% (many diffs)
51 human 728741 NPIPB6 nuclear pore complex intera... XM_024450419.1 15.6% 14.2% (many diffs)
52 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022825.2 15.3% 14% (many diffs)
53 human 728734 NPIPB8 nuclear pore complex intera... XM_017023626.1 15.3% 14% (many diffs)
54 human 728734 NPIPB8 nuclear pore complex intera... XM_024450416.1 15.3% 14% (many diffs)
55 human 728741 NPIPB6 nuclear pore complex intera... XM_017023628.1 15% 13.7% (many diffs)
56 human 728741 NPIPB6 nuclear pore complex intera... NM_001282524.1 14.9% 13.6% (many diffs)
57 human 728741 NPIPB6 nuclear pore complex intera... XM_011545929.2 14.9% 13.6% (many diffs)
58 human 100507607 NPIPB9 nuclear pore complex intera... NM_001287251.1 14.8% 13.5% (many diffs)
59 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022824.2 14.8% 13.5% (many diffs)
60 human 728734 NPIPB8 nuclear pore complex intera... XM_017023625.1 14.8% 13.5% (many diffs)
61 human 100507607 NPIPB9 nuclear pore complex intera... NM_001287250.1 14.7% 13.4% (many diffs)
62 human 728734 NPIPB8 nuclear pore complex intera... NM_001310136.1 14.7% 13.4% (many diffs)
63 human 100507607 NPIPB9 nuclear pore complex intera... XM_003118719.8 14.7% 13.4% (many diffs)
64 human 728734 NPIPB8 nuclear pore complex intera... XM_017023624.1 14.7% 13.4% (many diffs)
65 human 728734 NPIPB8 nuclear pore complex intera... XM_024450415.1 14.7% 13.4% (many diffs)
66 human 440348 NPIPB15 nuclear pore complex intera... NM_001306094.1 14.2% 12.8% (many diffs)
67 human 440348 NPIPB15 nuclear pore complex intera... XM_011523498.3 14.2% 12.8% (many diffs)
68 human 440348 NPIPB15 nuclear pore complex intera... XM_017023248.1 14.2% 12.8% (many diffs)
69 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022823.2 14.1% 12.8% (many diffs)
70 human 728734 NPIPB8 nuclear pore complex intera... XM_017023623.1 13.8% 12.6% (many diffs)
71 human 100507607 NPIPB9 nuclear pore complex intera... XM_011545693.3 13.8% 12.5% (many diffs)
72 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022820.2 13.7% 12.5% (many diffs)
73 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022821.2 13.7% 12.5% (many diffs)
74 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022822.2 13.7% 12.5% (many diffs)
75 human 440348 NPIPB15 nuclear pore complex intera... XM_011523496.2 13.7% 12.4% (many diffs)
76 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022819.2 13.3% 12.1% (many diffs)
77 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022818.2 13.2% 12.1% (many diffs)
78 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022817.2 13.2% 12% (many diffs)
79 human 728734 NPIPB8 nuclear pore complex intera... XM_017023622.1 13.2% 12% (many diffs)
80 human 100507607 NPIPB9 nuclear pore complex intera... XM_011545690.3 13.1% 11.9% (many diffs)
81 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022816.2 13% 11.8% (many diffs)
82 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022814.2 12.4% 11.3% (many diffs)
83 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022815.2 12.4% 11.3% (many diffs)
84 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756353.1 10% 1_847del;953_954ins99
85 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756354.2 7.3% (many diffs)
86 human 440353 NPIPB12 nuclear pore complex intera... NM_001355401.1 7.1% 6.7% (many diffs)
87 human 440345 NPIPB4 nuclear pore complex intera... XM_024450289.1 7% 6.6% (many diffs)
88 human 440345 NPIPB4 nuclear pore complex intera... XM_024450287.1 6.3% 5.9% (many diffs)
89 human 440345 NPIPB4 nuclear pore complex intera... XM_024450288.1 6.3% 5.9% (many diffs)
90 human 440345 NPIPB4 nuclear pore complex intera... XM_024450286.1 6.2% 5.9% (many diffs)
91 human 613037 NPIPB13 nuclear pore complex intera... NM_001321892.1 5.8% 5.5% (many diffs)
92 human 100132247 NPIPB5 nuclear pore complex intera... NM_001135865.1 5.8% 5.4% (many diffs)
93 human 23117 NPIPB3 nuclear pore complex intera... NM_130464.3 5.8% 5.4% (many diffs)
94 human 728888 NPIPB11 nuclear pore complex intera... NM_001310137.2 5.8% 5.4% (many diffs)
95 human 440345 NPIPB4 nuclear pore complex intera... XM_017023247.1 5.7% 5.4% (many diffs)
96 human 440345 NPIPB4 nuclear pore complex intera... XM_024450285.1 5.7% 5.4% (many diffs)
97 human 728888 NPIPB11 nuclear pore complex intera... XM_024450420.1 5.6% 5.2% (many diffs)
98 human 440345 NPIPB4 nuclear pore complex intera... XM_024450284.1 5.4% 5% (many diffs)
99 human 440345 NPIPB4 nuclear pore complex intera... NM_001310148.1 5.4% 5% (many diffs)
100 human 440345 NPIPB4 nuclear pore complex intera... XM_017023244.2 5.4% 5% (many diffs)
101 human 440345 NPIPB4 nuclear pore complex intera... XM_024450283.1 5.2% 4.9% (many diffs)
102 human 100288332 NPIPA5 nuclear pore complex intera... XR_932754.3 5.1% 1_1836del;1942_1943ins99
103 human 100288332 NPIPA5 nuclear pore complex intera... XR_002957772.1 4.9% 1_1159del;1227_1228ins137
104 human 283970 PDXDC2P-NPIPB14P nuclear pore complex-intera... NR_003610.1 4.4% (many diffs)
105 human 105369154 PKD1P6-NPIPP1 PKD1P6-NPIPP1 readthrough NR_123721.1 3.7% (many diffs)
106 human 339044 PKD1P1 polycystin 1, transient rec... NR_036447.1 3.2% 1_5784del;5987C>A;5990_6295del
107 human 110006323 PKD1P4-NPIPA8 PKD1P4-NPIPA8 readthrough NR_146336.1 2.8% 1_6591del;6794C>A;6797_7102del
108 human 109951030 PKD1P3-NPIPA1 PKD1P3-NPIPA1 readthrough NR_146231.1 2.7% (many diffs)
109 human 110006322 PKD1P5-LOC105376752 PKD1P5-LOC105376752 readthr... NR_146331.1 1.7% 1_11269del;11472C>A;11475_11747del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
297
ORF length:
207
Sequence:
1TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT
61GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGGCAGT GGAGCATCGT CATTCTTCAG
121GATTGCCCTA CTGGCCCTAC CTCACAGCTG AAACTTTAAA AAACAGGATG GGCCACCAGC
181CACCTCCTCC AACTCAACAA CATTCTATAA TTGATAACTC CCTGAGCCTC AAGACACCTT
241CCGAGTGTGT GCTCTATCCC CTTCCACCCT CAGCGGATGA TAATCTCAAG AAACTACCCA
301ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC
361GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT
421ATATCTTGTG GAAAGGACGA CACCCATAGA AGAATTACCG CCGAACGCGT TAAGTCgaca
481atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)

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