Construct: ORF TRCN0000465924

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018123.1_s317c1
Derived from:
ccsbBroadEn_09386
DNA Barcode:
GTCATGGAGAAAGTCGGTGACCGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBA3D (113457)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465924
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 113457 TUBA3D tubulin alpha 3d NM_080386.4 99.9% 99.7% 914G>A
2 human 112714 TUBA3E tubulin alpha 3e NM_207312.3 98.7% 98.4% (many diffs)
3 human 7278 TUBA3C tubulin alpha 3c NM_006001.3 96.5% 99.7% (many diffs)
4 human 10376 TUBA1B tubulin alpha 1b NM_006082.3 84.9% 96.8% (many diffs)
5 human 7846 TUBA1A tubulin alpha 1a NM_001270399.1 84.4% 97.3% (many diffs)
6 human 7846 TUBA1A tubulin alpha 1a NM_006009.4 84.4% 97.3% (many diffs)
7 human 7277 TUBA4A tubulin alpha 4a NM_006000.3 84% 94% (many diffs)
8 human 84790 TUBA1C tubulin alpha 1c NM_032704.5 83.2% 96% (many diffs)
9 human 7277 TUBA4A tubulin alpha 4a NM_001278552.1 81.1% 90.8% (many diffs)
10 human 51807 TUBA8 tubulin alpha 8 NM_018943.3 80.3% 90.4% (many diffs)
11 human 7846 TUBA1A tubulin alpha 1a NM_001270400.1 77.6% 89.5% (many diffs)
12 human 84790 TUBA1C tubulin alpha 1c NM_001303115.2 76.5% 88.2% (many diffs)
13 human 84790 TUBA1C tubulin alpha 1c NM_001303116.2 76.5% 88.2% (many diffs)
14 human 84790 TUBA1C tubulin alpha 1c NM_001303117.2 76.5% 88.2% (many diffs)
15 human 84790 TUBA1C tubulin alpha 1c NM_001303114.1 72% 82.8% (many diffs)
16 human 51807 TUBA8 tubulin alpha 8 NM_001193414.2 70.3% 79.3% (many diffs)
17 human 7277 TUBA4A tubulin alpha 4a XM_017004824.1 56.2% 63.1% (many diffs)
18 mouse 22147 Tuba3b tubulin, alpha 3B NM_009449.3 90.5% 99.7% (many diffs)
19 mouse 22144 Tuba3a tubulin, alpha 3A NM_009446.3 90.3% 99.7% (many diffs)
20 mouse 22143 Tuba1b tubulin, alpha 1B NM_011654.2 85.5% 96.8% (many diffs)
21 mouse 22142 Tuba1a tubulin, alpha 1A NM_011653.2 85.4% 97.3% (many diffs)
22 mouse 22146 Tuba1c tubulin, alpha 1C NM_009448.4 85.2% 96.2% (many diffs)
23 mouse 22145 Tuba4a tubulin, alpha 4A NM_009447.4 83.1% 94% (many diffs)
24 mouse 22145 Tuba4a tubulin, alpha 4A XM_006496478.1 80.2% 90.8% (many diffs)
25 mouse 53857 Tuba8 tubulin, alpha 8 NM_017379.2 78.8% 90.6% (many diffs)
26 mouse 22143 Tuba1b tubulin, alpha 1B XM_006520756.1 78.1% 88.2% (many diffs)
27 mouse 22145 Tuba4a tubulin, alpha 4A NM_001313724.1 54.6% 63.1% (many diffs)
28 mouse 22145 Tuba4a tubulin, alpha 4A NM_001313723.1 45.3% 52.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1416
ORF length:
1350
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg cgagtgtatc tctatccacg tggggcaggc gggtgtccag atcggcaatg
121cctgctggga actgtactgc cttgaacatg gaattcagcc cgatggccaa atgccaagtg
181ataaaaccat tggtggcggg gacgactcct tcaacacgtt cttcagtgag actggagctg
241gcaagcacgt gcccagagca gtgtttgtgg acctggagcc cactgtggtc gatgaagtgc
301gcacagggac ctacaggcag ctcttccacc cggagcagct gatcaccggg aaggaagatg
361cagccaataa ttacgccagg ggccattaca ccatcggcaa ggagattgtt gacctagtcc
421tggaccggat ccgcaaactg gcggatctgt gcacaggact gcagggcttc ctcatcttcc
481acagctttgg gggcggcact ggctctgggt tcgcatctct gctcatggag cggctctcag
541tggattacgg caagaagtcc aagctagagt ttgccattta cccagccccc caggtctcca
601cagccgtggt ggagccctac aactccatcc tgaccaccca cacgaccctg gaacattctg
661actgtgcctt catggtcgac aatgaagcca tctatgacat atgtcggcgc aacctggaca
721ttgaacgtcc cacgtacacc aacctcaatc gcctgattgg gcagatcgtg tcctccatca
781cagcctccct gcgatttgat ggggccctga atgtggactt gacggaattc cagaccaacc
841tagtgccgta cccccgcatc cacttccccc tggccaccta tgccccagtc atctcagctg
901agaaggccta ccacgagcag ctgtctgtgg ccgagatcac caatgcctgc ttcgagccag
961ccaatcaaat ggtcaagtat gaccctcgcc acggcaagta catggcctgc tgcatgttgt
1021acagggggga cgtggTCCCC AAAGACGTCA ACGCGGCCAT CGCCACCATC AAGACCAAGC
1081GCACCATCCA GTTTGTGGAT TGGTGCCCGA CTGGATTTAA GGTGGGCATT AACTACCAGC
1141CCCCCACAGT GGTCCCCGGG GGAGACCTGG CCAAGGTGCA GCGGGCCGTG TGCATGCTGA
1201GCAACACCAC GGCCATTGCG GAGGCCTGGG CCCGCCTGGA CCATAAGTTC GATCTCATGT
1261ATGCCAAGCG GGCCTTTGTG CACTGGTACG TGGGCGAAGG CATGGAAGAG GGAGAGTTCT
1321CTGAGGCCCG CGAGGACCTG GCAGCTCTAG AGAAGGATTA TGAAGAGGTG GGCGTGGATT
1381CCGTGGAAGC TGAGGCTGAA GAAGGCGAAG AATACTGCCC AACTTTCTTG TACAAAGTGG
1441TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1501CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1561AGTCATGGAG AAAGTCGGTG ACCGGACGCG TTAAGTCgac aatcaacctc tggattacaa
1621aatttgtgaa agatt

Download FASTA (ORF) (Full)