Construct: ORF TRCN0000465924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018123.1_s317c1
- Derived from:
- ccsbBroadEn_09386
- DNA Barcode:
- GTCATGGAGAAAGTCGGTGACCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBA3D (113457)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113457 | TUBA3D | tubulin alpha 3d | NM_080386.4 | 99.9% | 99.7% | 914G>A |
| 2 | human | 112714 | TUBA3E | tubulin alpha 3e | NM_207312.3 | 98.7% | 98.4% | (many diffs) |
| 3 | human | 7278 | TUBA3C | tubulin alpha 3c | NM_006001.3 | 96.5% | 99.7% | (many diffs) |
| 4 | human | 10376 | TUBA1B | tubulin alpha 1b | NM_006082.3 | 84.9% | 96.8% | (many diffs) |
| 5 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270399.1 | 84.4% | 97.3% | (many diffs) |
| 6 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_006009.4 | 84.4% | 97.3% | (many diffs) |
| 7 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_006000.3 | 84% | 94% | (many diffs) |
| 8 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_032704.5 | 83.2% | 96% | (many diffs) |
| 9 | human | 7277 | TUBA4A | tubulin alpha 4a | NM_001278552.1 | 81.1% | 90.8% | (many diffs) |
| 10 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_018943.3 | 80.3% | 90.4% | (many diffs) |
| 11 | human | 7846 | TUBA1A | tubulin alpha 1a | NM_001270400.1 | 77.6% | 89.5% | (many diffs) |
| 12 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303115.2 | 76.5% | 88.2% | (many diffs) |
| 13 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303116.2 | 76.5% | 88.2% | (many diffs) |
| 14 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303117.2 | 76.5% | 88.2% | (many diffs) |
| 15 | human | 84790 | TUBA1C | tubulin alpha 1c | NM_001303114.1 | 72% | 82.8% | (many diffs) |
| 16 | human | 51807 | TUBA8 | tubulin alpha 8 | NM_001193414.2 | 70.3% | 79.3% | (many diffs) |
| 17 | human | 7277 | TUBA4A | tubulin alpha 4a | XM_017004824.1 | 56.2% | 63.1% | (many diffs) |
| 18 | mouse | 22147 | Tuba3b | tubulin, alpha 3B | NM_009449.3 | 90.5% | 99.7% | (many diffs) |
| 19 | mouse | 22144 | Tuba3a | tubulin, alpha 3A | NM_009446.3 | 90.3% | 99.7% | (many diffs) |
| 20 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | NM_011654.2 | 85.5% | 96.8% | (many diffs) |
| 21 | mouse | 22142 | Tuba1a | tubulin, alpha 1A | NM_011653.2 | 85.4% | 97.3% | (many diffs) |
| 22 | mouse | 22146 | Tuba1c | tubulin, alpha 1C | NM_009448.4 | 85.2% | 96.2% | (many diffs) |
| 23 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_009447.4 | 83.1% | 94% | (many diffs) |
| 24 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | XM_006496478.1 | 80.2% | 90.8% | (many diffs) |
| 25 | mouse | 53857 | Tuba8 | tubulin, alpha 8 | NM_017379.2 | 78.8% | 90.6% | (many diffs) |
| 26 | mouse | 22143 | Tuba1b | tubulin, alpha 1B | XM_006520756.1 | 78.1% | 88.2% | (many diffs) |
| 27 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313724.1 | 54.6% | 63.1% | (many diffs) |
| 28 | mouse | 22145 | Tuba4a | tubulin, alpha 4A | NM_001313723.1 | 45.3% | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cgagtgtatc tctatccacg tggggcaggc gggtgtccag atcggcaatg 121 cctgctggga actgtactgc cttgaacatg gaattcagcc cgatggccaa atgccaagtg 181 ataaaaccat tggtggcggg gacgactcct tcaacacgtt cttcagtgag actggagctg 241 gcaagcacgt gcccagagca gtgtttgtgg acctggagcc cactgtggtc gatgaagtgc 301 gcacagggac ctacaggcag ctcttccacc cggagcagct gatcaccggg aaggaagatg 361 cagccaataa ttacgccagg ggccattaca ccatcggcaa ggagattgtt gacctagtcc 421 tggaccggat ccgcaaactg gcggatctgt gcacaggact gcagggcttc ctcatcttcc 481 acagctttgg gggcggcact ggctctgggt tcgcatctct gctcatggag cggctctcag 541 tggattacgg caagaagtcc aagctagagt ttgccattta cccagccccc caggtctcca 601 cagccgtggt ggagccctac aactccatcc tgaccaccca cacgaccctg gaacattctg 661 actgtgcctt catggtcgac aatgaagcca tctatgacat atgtcggcgc aacctggaca 721 ttgaacgtcc cacgtacacc aacctcaatc gcctgattgg gcagatcgtg tcctccatca 781 cagcctccct gcgatttgat ggggccctga atgtggactt gacggaattc cagaccaacc 841 tagtgccgta cccccgcatc cacttccccc tggccaccta tgccccagtc atctcagctg 901 agaaggccta ccacgagcag ctgtctgtgg ccgagatcac caatgcctgc ttcgagccag 961 ccaatcaaat ggtcaagtat gaccctcgcc acggcaagta catggcctgc tgcatgttgt 1021 acagggggga cgtggTCCCC AAAGACGTCA ACGCGGCCAT CGCCACCATC AAGACCAAGC 1081 GCACCATCCA GTTTGTGGAT TGGTGCCCGA CTGGATTTAA GGTGGGCATT AACTACCAGC 1141 CCCCCACAGT GGTCCCCGGG GGAGACCTGG CCAAGGTGCA GCGGGCCGTG TGCATGCTGA 1201 GCAACACCAC GGCCATTGCG GAGGCCTGGG CCCGCCTGGA CCATAAGTTC GATCTCATGT 1261 ATGCCAAGCG GGCCTTTGTG CACTGGTACG TGGGCGAAGG CATGGAAGAG GGAGAGTTCT 1321 CTGAGGCCCG CGAGGACCTG GCAGCTCTAG AGAAGGATTA TGAAGAGGTG GGCGTGGATT 1381 CCGTGGAAGC TGAGGCTGAA GAAGGCGAAG AATACTGCCC AACTTTCTTG TACAAAGTGG 1441 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1501 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1561 AGTCATGGAG AAAGTCGGTG ACCGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1621 aatttgtgaa agatt